ID E6R8Q5_CRYGW Unreviewed; 1463 AA.
AC E6R8Q5;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 24-JAN-2024, entry version 63.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN OrderedLocusNames=CGB_F6200C {ECO:0000313|EMBL:ADV23207.1};
OS Cryptococcus gattii serotype B (strain WM276 / ATCC MYA-4071)
OS (Filobasidiella gattii) (Cryptococcus bacillisporus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus gattii species complex.
OX NCBI_TaxID=367775 {ECO:0000313|EMBL:ADV23207.1, ECO:0000313|Proteomes:UP000007805};
RN [1] {ECO:0000313|EMBL:ADV23207.1, ECO:0000313|Proteomes:UP000007805}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WM276 / ATCC MYA-4071 {ECO:0000313|Proteomes:UP000007805};
RX PubMed=21304167; DOI=10.1128/mBio.00342-10;
RA D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G.,
RA Jung W.H., Sham A., Kidd S.E., Tangen K., Lee N., Zeilmaker T., Sawkins J.,
RA McVicker G., Shah S., Gnerre S., Griggs A., Zeng Q., Bartlett K., Li W.,
RA Wang X., Heitman J., Stajich J.E., Fraser J.A., Meyer W., Carter D.,
RA Schein J., Krzywinski M., Kwon-Chung K.J., Varma A., Wang J., Brunham R.,
RA Fyfe M., Ouellette B.F., Siddiqui A., Marra M., Jones S., Holt R.,
RA Birren B.W., Galagan J.E., Cuomo C.A.;
RT "Genome variation in Cryptococcus gattii, an emerging pathogen of
RT immunocompetent hosts.";
RL MBio 2:E342-E342(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WM276;
RA D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G.,
RA Jung W.H., Sham A., Kidd S.E., Tangen K., Lee N., Zeilmaker T., Sawkins J.,
RA McVicker G., Shah S., Gnerre S., Griggs A., Zeng Q., Bartlett K., Li W.,
RA Wang X., Heitman J., Stajich J.E., Fraser J.A., Meyer W., Carter D.,
RA Schein J., Krzywinski M., Kwong-Chung K.J., Varma A., Wang J., Brunham R.,
RA Fyfe M., Ouellette B.F.F., Siddiqui A., Marra M., Jones S., Holt R.,
RA Birren B.W., Galagan J.E., Cuomo C.A.;
RT "Genome variation in Cryptococcus gattii, an emerging pathogen of
RT immunocompetent hosts.";
RL MBio 0:0-0(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
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DR EMBL; CP000291; ADV23207.1; -; Genomic_DNA.
DR RefSeq; XP_003194994.1; XM_003194946.1.
DR GeneID; 10190624; -.
DR KEGG; cgi:CGB_F6200C; -.
DR VEuPathDB; FungiDB:CGB_F6200C; -.
DR eggNOG; KOG0261; Eukaryota.
DR HOGENOM; CLU_000487_3_0_1; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000007805; Chromosome F.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02736; RNAP_III_Rpc1_C; 1.
DR CDD; cd02583; RNAP_III_RPC1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR035698; RNAP_III_Rpc1_C.
DR InterPro; IPR035697; RNAP_III_RPC1_N.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 256..564
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 971..1000
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1463 AA; 162821 MW; 3EAF55EA45181547 CRC64;
MDTWAVVDQN EKHYVSSDVP RRIKHIQFQP FTPKDIVRIS EVQVSIAELY QNNEDGSRTT
ALQGPLDARM GPNEKGKKCA TCGEDTQKCV GHYGYIKLVL PVFHIGYFRP TINMLSCICK
TCARVLLPET ERASFLKRFR RPNLESLQRQ SAAKAVLAAC KKQNICTYCG APNGIVKKSG
PLRISHEPYR AAKMASMKDE WMATFKNAVA ENQAVATHLG KAVDDLNPLK VLNLFERVTA
EDCELLSLHP DVGRPEDYIW QYISVPPPCI RPSVASEAGN NEDDLTAKLA EIVNYNNTLA
LMMDLGRGIE MISSNWEVLG QAVALYINSQ APGMQTIGSK PIRGFVQRLK GKQGRFRGNL
SGKRVDFSGR TVIGPDPNLR IDEVAVPEKV AVKLSYPERV TDYNLQLMRQ AIINGSKLHP
GANVLERKHE NGSTVRISLN VMKDLEARKR YARELQIGDV VHRHVRDGDI VLFNRQPSLH
KLSIMCHRVR VRPWRTFRLN ECVCNPYNAD FDGDEMNLHV PQTEEARTEA LELMSVKKNL
VTPRNGEPII AAIQDFITAA YLLSRRDRFY DRQQFTQIAS YLGDANLKIE MPPPTIWKPV
RLWTGKQIFN LLMRPNKESK VLVNLEAKCR TLVDPAKEDR FPPDMSPNDG YLVIQNSEIM
CGVFDKNTVG DGKKNSVFGV ILRDYGPEEA AQAMNRLAKI AARWLANIGF SLGINDVIPG
PILHASKDAR VEKAYGECDV FIDLAKRGKL ENAPGCDQEA TLESKISKTL SDVREAVGKI
CMQELSRHNA PLIMATCGSK GSVINVAQMV ACVGQQIIGG SRVRNGFQDR SLPHFRKKSK
NPPSKGFVRN SFFSGLTPPE FLFHAMSGRE GLVDTAVKTA ETGYMARRLM KALEDLCTHY
DLSVRNSVGG IVQFQYGDDM LDPACLEGDA TPVEYVRSWT HACRTAGNQG RALFPYEVRR
IAARIYPESF ILPSPPPSPP KTKGRRRKEP TPPPPVVAPA LPKEWEGCHH KFRENTYKFV
VDKIVKQMVD QRLARGLPDA DDEEMAEEYE ALLYSDDPAV QRVLENANKV TEAQVNSFLE
NCRIRYLRAK IEPGSTVGAV GAQSIGEPGT QMTLKTFHFA GVASMNVTLG VPRIKEIINA
AKVISTPIIA AELAIPESET AARIVKGRIE KTVLGDIAAV IEESWTNANA YIEVHIDMDA
VRRLQLEISL ESIKWALVRA NKLKIPEGSI HISQKTSRIR IWIDESDKDK GVGIYERLKF
LKRAIPSVQV KGLPAIERGV VTKDEKNEKI HRLLVTGYGL SEVMGTEGVD GLKTKTNHVM
ETQQVLGIEA ARSTIYNEIQ TTMKSHGMSI DPRHVMLLGD VMTYKGEVLG ITRFGVQKMK
DSVLMLASFE KTTDHLFDAS LYSKKDEIQG VSECIIMGTP APGCGTSLAS IVTPAPPLPA
KKPLLFETAY KAGQHRMSKI AAC
//
