ID E6R8V6_CRYGW Unreviewed; 2431 AA.
AC E6R8V6;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=alpha-1,3-glucan synthase {ECO:0000256|ARBA:ARBA00012688};
DE EC=2.4.1.183 {ECO:0000256|ARBA:ARBA00012688};
GN OrderedLocusNames=CGB_G0300C {ECO:0000313|EMBL:ADV23282.1};
OS Cryptococcus gattii serotype B (strain WM276 / ATCC MYA-4071)
OS (Filobasidiella gattii) (Cryptococcus bacillisporus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus gattii species complex.
OX NCBI_TaxID=367775 {ECO:0000313|EMBL:ADV23282.1, ECO:0000313|Proteomes:UP000007805};
RN [1] {ECO:0000313|EMBL:ADV23282.1, ECO:0000313|Proteomes:UP000007805}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WM276 / ATCC MYA-4071 {ECO:0000313|Proteomes:UP000007805};
RX PubMed=21304167; DOI=10.1128/mBio.00342-10;
RA D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G.,
RA Jung W.H., Sham A., Kidd S.E., Tangen K., Lee N., Zeilmaker T., Sawkins J.,
RA McVicker G., Shah S., Gnerre S., Griggs A., Zeng Q., Bartlett K., Li W.,
RA Wang X., Heitman J., Stajich J.E., Fraser J.A., Meyer W., Carter D.,
RA Schein J., Krzywinski M., Kwon-Chung K.J., Varma A., Wang J., Brunham R.,
RA Fyfe M., Ouellette B.F., Siddiqui A., Marra M., Jones S., Holt R.,
RA Birren B.W., Galagan J.E., Cuomo C.A.;
RT "Genome variation in Cryptococcus gattii, an emerging pathogen of
RT immunocompetent hosts.";
RL MBio 2:E342-E342(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WM276;
RA D'Souza C.A., Kronstad J.W., Taylor G., Warren R., Yuen M., Hu G.,
RA Jung W.H., Sham A., Kidd S.E., Tangen K., Lee N., Zeilmaker T., Sawkins J.,
RA McVicker G., Shah S., Gnerre S., Griggs A., Zeng Q., Bartlett K., Li W.,
RA Wang X., Heitman J., Stajich J.E., Fraser J.A., Meyer W., Carter D.,
RA Schein J., Krzywinski M., Kwong-Chung K.J., Varma A., Wang J., Brunham R.,
RA Fyfe M., Ouellette B.F.F., Siddiqui A., Marra M., Jones S., Holt R.,
RA Birren B.W., Galagan J.E., Cuomo C.A.;
RT "Genome variation in Cryptococcus gattii, an emerging pathogen of
RT immunocompetent hosts.";
RL MBio 0:0-0(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC alpha-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:19749, Rhea:RHEA-
CC COMP:11150, Rhea:RHEA-COMP:11151, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28100, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.183; Evidence={ECO:0000256|ARBA:ARBA00000687};
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|ARBA:ARBA00006122}.
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DR EMBL; CP000292; ADV23282.1; -; Genomic_DNA.
DR RefSeq; XP_003195069.1; XM_003195021.1.
DR GeneID; 10190845; -.
DR KEGG; cgi:CGB_G0300C; -.
DR VEuPathDB; FungiDB:CGB_G0300C; -.
DR eggNOG; ENOG502QSGC; Eukaryota.
DR HOGENOM; CLU_000488_0_0_1; -.
DR OrthoDB; 141134at2759; -.
DR Proteomes; UP000007805; Chromosome G.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0047657; F:alpha-1,3-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11323; AmyAc_AGS; 1.
DR CDD; cd03791; GT5_Glycogen_synthase_DULL1-like; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR PANTHER; PTHR47182; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED; 1.
DR PANTHER; PTHR47182:SF2; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK13; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|SAM:Phobius}; Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..2431
FT /note="alpha-1,3-glucan synthase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003210081"
FT TRANSMEM 2041..2058
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2065..2084
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2104..2121
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2133..2154
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2166..2190
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2211..2231
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2251..2273
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2280..2300
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2315..2342
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2349..2369
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2403..2423
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 76..526
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1678..1719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1740..1802
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1817..1846
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1956..1986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1776..1802
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2431 AA; 272388 MW; 1701B2F25910FF59 CRC64;
MLLRPPREAR ISLFPLVLTL FATSLSVLGL KYNETLDAWN INKNQDATNV LDYTTTRSNR
TYTKSPDNWR SLPTYTLLLD KWIDGSPQNN DFFGLKYEYD MRETQLRAGG DAKGLMSDRS
LDYLLSMGYK AIYIAGTNFI NMPWQSDGYS ALDFTLLDPH YGTLQEWVDL VDRIHAKGMH
IILDFTVGTM GDLIGFEGHL NTSTPFSMDE HVAQWKLPDY APWGFQKYPD FNFTNDYNNS
CIYPTFWLDD GTIYDPGKVG CYASDFDQYG DIEAFGVFPD WQRQLAKFAS VQDRLREWRP
DTAAKLKRFS CLAIEALDID GIRIDKATQV TVDFMAEWAN STRECARQFN KDNFFIPGEI
TGGDTFGALY IGRGRTPTLQ PSNFTQAVEL TQDQGEYFLR GEGIAGLDSA AFHYSIYRSL
CRFLGMDGNL QVAYDVDVNF VTAWNEMVVS NEFLNSQTND FDPRHMYGTT NQDVFRWPGL
ANGTERQQLG QFVTVLLMPG IPLGWYGEEQ AFYLLDNTAS NYLYGRQAMV SNPAWKKHGC
YKVGSSQYYN MPYDKVLTGC DDPWNSLDHY DPTAEPLRGI RNMMYLRTRF PSLQDGFSLT
TLGNWTHFIQ LPGSNQTQTE LGMWSVTRAP IAQQEGHGSF EANGTISQAV WLIYTNENDT
VPYSPDCKQE GWISSPFQSG VTVRNLFYPY ENYTLQDSLS SYYADGNPPY RGCLGSITLD
PFMYKALVPQ DQWIPQLPTV TKFTPGHDYR YQTSGGTTNV DITIEYDTDM DCDSLRRAMT
LNYTIVGSGS PSLSDGTCQS IAQADWETPS IPSVAPGRWR WTGQLQNAPD GIYEIIISSV
DSQISGVPTN NTDHLLLRLG QTSNAIVFPE NDYSDSLLTV SDGVYTLNNT ADGADAYRYS
ADFGQTWSAW MNYETNPTLN ASMFDDDTHW WDGYHVVVQY YSNIAGSANH LIHGDANWAH
GHRRWPQLLA RGSFNTWGYD LGADSSFALV EDGTWEIPLS SAWPAYIQLN VWAFDDYFYG
DVDGDGVIDR LAPNAQSANY LNMSIPPAPH LAWNVIVDDA TGEWSVEPIG HQTTTAIAFA
LLIVIPALTA FAAAAIFRYS FYSIKVNRFG LKPNKASKET SYFPIIGGHS EKKGDVSEKG
ALALNEKHHK TPAKVIGWPE DQNKRRKVLI ATLEYEIMDW KLKVKIGGLG VMSSLMGKAM
TDVDLIWVVP KVQDLEYPQG EYAEPIEVII FGEPYLIEVE THKLDNITYV ILDSPVFRAQ
TKADPYPQRM DDLSSAIFYS TWNQAIAETI RRNPIVDIYH INDYHGALAP LYMLPKVVPV
CLSLHNAEFQ GLWPLRTKDE MKEVCAAFNI PKEVCSKYVQ FGNTFNLLHA AASFISHHQK
SVGVAGVSDK YGKRSWARYP ALWTLRNIDS LPNPDPTDIA ALDEQPLAVD KIEVDQEAES
KRPEQKRQAQ EWAGLKQDPD ADLFVFVGRW SKQKGVDLIA DVMPSLLDKK PKIQLICVGP
VIDLYGRFAA EKLARLMEMY PDRVYSKPEF TALPPYLFSG ADFALIPSRD EPFGLVAVEF
GRKGALGVGS RLGGLGLMPG WWFPVESSAT VHMLSQLTKT IKLALKSTPE ERAILRARSA
VQRFPVVEWR QRLEDFQRRS IIASRQGAGD HAWGYDLVDQ EVGSGFYAQG DNGSFTSLAR
GEWGRSATPD STAPNSPMPG HSPLAGEGQN FFDPNLPMAS SNSSLGANYH KRFDKNANRQ
SAESFYDEDP NASPLYYGDK RQSGKPKFFG YDDQDVASSV GSSDQGDTTV VGSTSGQRDP
SAQTYDNFLA AANRQFAKSS GERNAPDPYF SNRTSQDSTN FAPSRPFTAH SRVSSFDSIS
SIVDEKGSSP LNKAMETFTD ADGEVAQSFV QKLKDLSASN SKGDLCIEKF LIKSEKAFFD
EVKKDKIASM SIRSRDSFVQ SRAPSMIDGF RPDSPMSHSM SHSGHGHDEM SQYGGIPGGH
DMYDEGGHSE PRMTKLQIFM GRQIYRWPLY SIVLSLGQLL SATSFQLSLL GGSNTQTATD
LYIICSIFAV ATIAWYTLFR MKPSVYCLSL PWVIFTIAFL LIGFPSIHGV FLAPRLTITR
VATYMYAIAS SAGFLFFGLN FGEEAGAATE IWVTRACIVQ GLQQIWVSAL WYWGYTLNGS
DPDKYVPSRI IICICWPLAA VSAVFAYVMW FGLPEYYRQI PPYVPNFFKT LFRRKLVIWF
LIAEILRDYW LSGPYGRSWQ YLWSQAGVPK WAVVIMIAIF FVGIWGLLLG ILIRYSKIHS
WLLPVFAIGL GCPRWCQMWW GTSGMGLYIP WGGSAGPYIG VCTWLWLGVL DAIQGVGLGM
VLLQTLSRLH VCVTLALAQF VGSCVVMLAR GTAPDKVGPG KVFPNVAIWD IGGSGKNMPL
AHYEFWLCLV FQIIIVIGYA IFFRREQLSK P
//
