ID E6UI79_RUMA7 Unreviewed; 780 AA.
AC E6UI79;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=Rumal_1640 {ECO:0000313|EMBL:ADU22140.1};
OS Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 /
OS 7).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=697329 {ECO:0000313|EMBL:ADU22140.1, ECO:0000313|Proteomes:UP000006919};
RN [1] {ECO:0000313|EMBL:ADU22140.1, ECO:0000313|Proteomes:UP000006919}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7
RC {ECO:0000313|Proteomes:UP000006919};
RX PubMed=21914885; DOI=10.1128/JB.05621-11;
RA Suen G., Stevenson D.M., Bruce D.C., Chertkov O., Copeland A., Cheng J.F.,
RA Detter C., Detter J.C., Goodwin L.A., Han C.S., Hauser L.J., Ivanova N.N.,
RA Kyrpides N.C., Land M.L., Lapidus A., Lucas S., Ovchinnikova G.,
RA Pitluck S., Tapia R., Woyke T., Boyum J., Mead D., Weimer P.J.;
RT "Complete genome of the cellulolytic ruminal bacterium Ruminococcus albus
RT 7.";
RL J. Bacteriol. 193:5574-5575(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP002403; ADU22140.1; -; Genomic_DNA.
DR RefSeq; WP_013498305.1; NZ_JHYT01000031.1.
DR AlphaFoldDB; E6UI79; -.
DR STRING; 697329.Rumal_1640; -.
DR KEGG; ral:Rumal_1640; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000006919; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 2.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 17..338
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 339..611
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 780 AA; 88008 MW; 584371450347D87A CRC64;
MKDIDMIKKQ ALERFFSRMN PMQQKAVFRV NGPLLILAGA GSGKTTVLVN RIANMIHFGD
AYNTTGQIFT DEEAAFIKDY AEGRTDDGKR LAAVIGVRQV RPWNILAITF TNKAAGELKE
RIEKILGDEG RGIVAATFHS ACVRILRREC GNIGFTPSFA IYDTDDSKRV IKAAMRSLDI
DEKMFPVKTV MSEISHAKDS MITPEEFAKE ASGDYYRMNV AKIYKKYQKE LLANNAMDFD
DIICHTVTLF ENCPDVLDHY QNLYKYIMVD EYQDTNRVQF RLVSLLSQKF GNICVVGDDD
QSIYRFRGAT IENILNFEDE FGCRPEDVIK LEQNYRSTQN ILTCANKLIS NNVGRKGKNL
WTDCGDGDKV IVHKSMNERD EASYIAKTVS ENNDNGRRFG DHAVLYRMNA QSNALEQAMI
KAGIPYKIFG GLKFYDRKEI KDILAYLEVI NNHADMFRLQ RIVNEPKRGI GDATVRTIGQ
VSSDLGEDPI YVMAHSTEYV PLAKKSKALT EFAFMIEDLT DLSEEASLPE LLDEVVEKTG
YGAMMKQQGV EGEMRLENIA ELKSTMTKYE EEAEDPSLEG FLEEVALYTD IDTLDENSDY
VALMTMHAAK GLEFPVVFVA GMEDNVFPSS RSRDSEAELE EERRLAYVAM TRAKEQLHLI
YAGERMLYGN TQYNRESQFI KELPPENIEK QVPKLDTAAS SRRSGDISLG RQMAMFNSAK
AAPTEPAESY SVGERVMHKK FGEGTIIGVT PMAGDTMLEI AFEKVGTKKI FANFAKPKKI
//
