ID E6UKG4_RUMA7 Unreviewed; 1231 AA.
AC E6UKG4;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=Rumal_3723 {ECO:0000313|EMBL:ADU24160.1};
OS Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 /
OS 7).
OG Plasmid pRUMAL02 {ECO:0000313|EMBL:ADU24160.1,
OG ECO:0000313|Proteomes:UP000006919}.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=697329 {ECO:0000313|EMBL:ADU24160.1, ECO:0000313|Proteomes:UP000006919};
RN [1] {ECO:0000313|Proteomes:UP000006919}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7
RC {ECO:0000313|Proteomes:UP000006919};
RC PLASMID=pRUMAL02 {ECO:0000313|Proteomes:UP000006919};
RX PubMed=21914885; DOI=10.1128/JB.05621-11;
RA Suen G., Stevenson D.M., Bruce D.C., Chertkov O., Copeland A., Cheng J.F.,
RA Detter C., Detter J.C., Goodwin L.A., Han C.S., Hauser L.J., Ivanova N.N.,
RA Kyrpides N.C., Land M.L., Lapidus A., Lucas S., Ovchinnikova G.,
RA Pitluck S., Tapia R., Woyke T., Boyum J., Mead D., Weimer P.J.;
RT "Complete genome of the cellulolytic ruminal bacterium Ruminococcus albus
RT 7.";
RL J. Bacteriol. 193:5574-5575(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; CP002405; ADU24160.1; -; Genomic_DNA.
DR RefSeq; WP_013483705.1; NZ_JHYT01000016.1.
DR AlphaFoldDB; E6UKG4; -.
DR KEGG; ral:Rumal_3723; -.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000006919; Plasmid pRUMAL02.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Plasmid {ECO:0000313|EMBL:ADU24160.1}.
FT DOMAIN 3..490
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 510..812
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 24..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1231 AA; 139426 MW; 56F4D042A50335EC CRC64;
MSVSWTEDQQ KAIDSYGRGV TVSAAAGSGK TAVLIERIIR LLTDKEKAIP ADRLLAVTFT
IDAAAQMRDK LNAAFEKKLR EDPENVWVLQ QQDLAQLARI STIDSFCFDM VKENLNKFEF
TGNLKILSEA ECDMIFDTAF EQAAEELCAN DPDEYKFLDN FFPVENGDLK YAVKELYGHL
RSICHTDKWI RDTSDNCLSD EFFDMLYEST FSRFKGKLDE AARFLDDARY CYNYTISAGG
ASYALKDHVK TAEENMAICE AVFSSYQLAA AHLDSTAFGK IETEKYKPMR LSGKPPEDVK
AIFSDVKERF KNDINNVCTV LKEGSRGLEL SKEHLRDNLV LVQKLFMAVC RLEKRVEEVS
YDIKLEKNAV DFGDIELMTK NLLVRETDDG FERTELAEEI RSGGAYKLIV IDEYQDVNDI
QEMIFKAVSD TDDLTVMGSN TFIVGDMKQA IYGFRKTNPE LFKNCIELAQ RYGEEKKLEH
IGLQKNFRSR GEVIALSNFI FETLMSSGCG QVDYDGSERL EAGAVYTDRS CPAEVMLVDA
PDEKSGIPLE FRCTAKRIRE MIDSGEPVCD GGVDRPCRQS DFCILVNTND NIRQATDALS
AAGLRAFCED TEGYIKSREI SLALDLLRCV DNPMNDISLT AVMMSPVMGF TPDEMVHIRL
KCKAANPKKP DHIYQILAGA SREKGTDEKY AKYVDMGSDV LQEKCRKAFE MIESLRYCSM
SMSLEKLIRR IFNAADLMAL TSIYLDSAKK RANLRLLLQY AGDYERSGNE GVTGFLRYID
SVSGNDKAFR NAVSVTAGGD SVNVKTYHKS KGLEYPFVFM CTLSKPLVRD DTRELIKLHK
TMGCAFVLKD RRLNVKRADL YYDHLSDMIV REHKSERMRL FYVGCTRAKE KLMFVLSNEK
TGHVTRSRIM EKLRDAVKAS AKYDRIPADI VSAQENVLTW TVMCLSKLKE RSALEEWLGM
PLDTVPVSEN AEGISISYHV CSEDTDTEVS EQNEKTVRAA ADPALVARLR NIYAASADRP
ETRLPAKLTV TEIVAAEKAK DQGDKNTEFF PNLPRLNDEL DKLSAAERGT YTHKFMELAD
YSKARESVDA ELDRLVSRGC MSEREAKGVY TDKLKIFVNS SLFDRMMSSD DLRREQKFLA
SVKDLGLTEE LSEYTTNKGY IQGIADCIFK EPDGWVLVDY KTDNFKSTDE MKRYKTQLML
YKAAFELLLG ERVKSSYIYS FKLGEGLEFV L
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