ID E7EUN2_HUMAN Unreviewed; 1069 AA.
AC E7EUN2;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE SubName: Full=ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 {ECO:0000313|Ensembl:ENSP00000386897.1};
GN Name=AGAP1 {ECO:0000313|Ensembl:ENSP00000386897.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000386897.1, ECO:0000313|Proteomes:UP000005640};
RN [1] {ECO:0000313|Ensembl:ENSP00000386897.1, ECO:0000313|Proteomes:UP000005640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [2] {ECO:0007829|PubMed:18220336}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18220336; DOI=10.1021/pr0705441;
RA Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.D., Yates J.R.;
RT "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient
RT phosphoproteomic analysis.";
RL J. Proteome Res. 7:1346-1351(2008).
RN [3] {ECO:0007829|PubMed:23186163}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=23186163;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [4] {ECO:0007829|PubMed:24275569}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [5] {ECO:0000313|Ensembl:ENSP00000386897.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the centaurin gamma-like family.
CC {ECO:0000256|ARBA:ARBA00005430}.
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DR EMBL; AC012305; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC019047; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC064874; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC073989; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC079400; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; E7EUN2; -.
DR SMR; E7EUN2; -.
DR IntAct; E7EUN2; 14.
DR MassIVE; E7EUN2; -.
DR MaxQB; E7EUN2; -.
DR ProteomicsDB; 18467; -.
DR Antibodypedia; 34463; 159 antibodies from 25 providers.
DR Ensembl; ENST00000409538.5; ENSP00000386897.1; ENSG00000157985.20.
DR UCSC; uc061txv.1; human.
DR HGNC; HGNC:16922; AGAP1.
DR VEuPathDB; HostDB:ENSG00000157985; -.
DR GeneTree; ENSGT00940000154793; -.
DR HOGENOM; CLU_007326_5_0_1; -.
DR PhylomeDB; E7EUN2; -.
DR ChiTaRS; AGAP1; human.
DR Proteomes; UP000005640; Chromosome 2.
DR Bgee; ENSG00000157985; Expressed in frontal pole and 199 other cell types or tissues.
DR ExpressionAtlas; E7EUN2; baseline and differential.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd08853; ArfGap_AGAP2; 1.
DR CDD; cd04103; Centaurin_gamma; 1.
DR CDD; cd01250; PH_AGAP; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 1.10.220.150; Arf GTPase activating protein; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR001806; Small_GTPase.
DR PANTHER; PTHR45819:SF1; ARF-GAP WITH GTPASE, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1; 1.
DR PANTHER; PTHR45819; CENTAURIN-GAMMA-1A; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF01412; ArfGap; 1.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00105; ArfGap; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00175; RAB; 1.
DR SMART; SM00173; RAS; 1.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF57863; ArfGap/RecO-like zinc finger; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50115; ARFGAP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS51419; RAB; 1.
DR PROSITE; PS51421; RAS; 1.
PE 1: Evidence at protein level;
KW ANK repeat {ECO:0000256|ARBA:ARBA00023043, ECO:0000256|PROSITE-
KW ProRule:PRU00023}; GTPase activation {ECO:0000256|ARBA:ARBA00022468};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Proteomics identification {ECO:0007829|EPD:E7EUN2,
KW ECO:0007829|MaxQB:E7EUN2};
KW Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00288}.
FT DOMAIN 611..800
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 821..941
FT /note="Arf-GAP"
FT /evidence="ECO:0000259|PROSITE:PS50115"
FT REPEAT 980..1012
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REGION 1..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..608
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 669..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..561
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 583..602
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 683..724
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1069 AA; 116619 MW; A3ECB976722085F7 CRC64;
MYLLDGSGEP ASPPADAMPR GTPPRKTVYR ISVTMVRKEQ LAAPGSGGPD PRPVRRPRPA
RSPDAPGRLE EEAEEAEGAE EPEGPRPRAW DFRTFRTRST GQLELGRLRP CARGLEPADL
AGSAPAEEEG RGSPASGSPD VEGARAAPLR RSLSFRHWSG PEAPQGRTLG GGRRHSSSGS
LAWAPCDEAA AGTTLEPATA TQPASEKRNT LDVGEVLSKN DALSDLERWE RSKSKNRTLD
NSDLQRLERA RAAAGAGAAS EHRLLRFFSG IFARRDGGPG GGPSPRSGAS RPRGYFSLRR
APAEAHSSSA ESIDGSPRRD AFVNSQEWTL SRSVPELKVG IVGNLASGKS ALVHRYLTGT
YVQEESPEGG RFKKEIVVDG QSYLLLIRDE GGPPEAQFAM WVDAVIFVFS LEDEISFQTV
YHYYSRMANY RNTSEIPLVL VGTQDAISSA NPRVIDDARA RKLSNDLKRC TYYETCATYG
LNVERVFQDV AQKIVATRKK QQLSIGPCKS LPNSPSHSSV CSAQVSAVHI SQTSNGGGSL
SDYSSSVPST PSTSQKELRI DVPPTANTPT PVRKQSKRRS NLFTSRKGSD PDKEKKGLES
RADSIGSGRA IPIKQGMLLK RSGKSLNKEW KKKYVTLCDN GVLTYHPSLH DYMQNVHGKE
IDLLRTTVKV PGKRPPRATS ACAPISSPKT NGLSKDMSSL HISPNSDTGL GDSVCSSPSI
SSTTSPKLDP PPSPHANRKK HRRKKSTSNF KADGLSGTAE EQEENFEFII VSLTGQTWHF
EATTYEERDA WVQAIESQIL ASLQSCESSK NKSRLTSQSE AMALQSIRNM RGNSHCVDCE
TQNPNWASLN LGALMCIECS GIHRNLGTHL SRVRSLDLDD WPVELIKVMS SIGNELANSV
WEESSQGRTK PSVDSTREEK ERWIRAKYEQ KLFLAPLPCT ELSLGQHLLR ATADEDLRTA
ILLLAHGSRD EVNETCGEGD GRTALHLACR KGNVVLAQLL IWYGVDVTAR DAHGNTALAY
ARQASSQECI DVLLQYGCPD ERFVLMATPN LSRRNNNRNN SSGRVPTII
//
