ID E7N9A4_9ACTO Unreviewed; 290 AA.
AC E7N9A4;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=Formyltetrahydrofolate deformylase {ECO:0000256|HAMAP-Rule:MF_01927};
DE EC=3.5.1.10 {ECO:0000256|HAMAP-Rule:MF_01927};
DE AltName: Full=Formyl-FH(4) hydrolase {ECO:0000256|HAMAP-Rule:MF_01927};
GN Name=purU {ECO:0000256|HAMAP-Rule:MF_01927,
GN ECO:0000313|EMBL:EFW27243.1};
GN ORFNames=FBF36_03940 {ECO:0000313|EMBL:QCT32724.1}, HMPREF9057_01366
GN {ECO:0000313|EMBL:EFW27243.1};
OS Actinomyces sp. oral taxon 171 str. F0337.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Actinomyces.
OX NCBI_TaxID=706439 {ECO:0000313|EMBL:EFW27243.1, ECO:0000313|Proteomes:UP000005722};
RN [1] {ECO:0000313|EMBL:EFW27243.1, ECO:0000313|Proteomes:UP000005722}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0337 {ECO:0000313|EMBL:EFW27243.1,
RC ECO:0000313|Proteomes:UP000005722};
RA Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B., Courtney L.,
RA Fronick C., Harrison M., Strong C., Farmer C., Delahaunty K., Markovic C.,
RA Hall O., Minx P., Tomlinson C., Mitreva M., Hou S., Chen J., Wollam A.,
RA Pepin K.H., Johnson M., Bhonagiri V., Zhang X., Suruliraj S., Warren W.,
RA Chinwalla A., Mardis E.R., Wilson R.K.;
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:QCT32724.1, ECO:0000313|Proteomes:UP000310751}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0337 {ECO:0000313|EMBL:QCT32724.1,
RC ECO:0000313|Proteomes:UP000310751};
RA Collins A.J., Murugkar P., Chen T., Dewhirst F.E.;
RT "Saccharibacteria TM7 and host bacteria genomes.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the hydrolysis of 10-formyltetrahydrofolate
CC (formyl-FH4) to formate and tetrahydrofolate (FH4). {ECO:0000256|HAMAP-
CC Rule:MF_01927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6R)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-
CC tetrahydrofolate + formate + H(+); Xref=Rhea:RHEA:19833,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366; EC=3.5.1.10;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01927};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
CC {ECO:0000256|HAMAP-Rule:MF_01927}.
CC -!- SIMILARITY: Belongs to the PurU family. {ECO:0000256|HAMAP-
CC Rule:MF_01927}.
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DR EMBL; AECW01000200; EFW27243.1; -; Genomic_DNA.
DR EMBL; CP040005; QCT32724.1; -; Genomic_DNA.
DR RefSeq; WP_009395617.1; NZ_GL637808.1.
DR AlphaFoldDB; E7N9A4; -.
DR eggNOG; COG0788; Bacteria.
DR HOGENOM; CLU_038395_3_0_11; -.
DR UniPathway; UPA00074; UER00170.
DR Proteomes; UP000005722; Unassembled WGS sequence.
DR Proteomes; UP000310751; Chromosome.
DR GO; GO:0008864; F:formyltetrahydrofolate deformylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04875; ACT_F4HF-DF; 1.
DR CDD; cd08648; FMT_core_Formyl-FH4-Hydrolase_C; 1.
DR Gene3D; 3.30.70.260; -; 1.
DR Gene3D; 3.40.50.170; Formyl transferase, N-terminal domain; 1.
DR HAMAP; MF_01927; PurU; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR041729; Formyl-FH4-Hydrolase_C.
DR InterPro; IPR002376; Formyl_transf_N.
DR InterPro; IPR036477; Formyl_transf_N_sf.
DR InterPro; IPR004810; PurU.
DR InterPro; IPR044074; PurU_ACT.
DR NCBIfam; TIGR00655; PurU; 1.
DR PANTHER; PTHR42706; FORMYLTETRAHYDROFOLATE DEFORMYLASE; 1.
DR PANTHER; PTHR42706:SF1; FORMYLTETRAHYDROFOLATE DEFORMYLASE 1, MITOCHONDRIAL; 1.
DR Pfam; PF01842; ACT; 1.
DR Pfam; PF00551; Formyl_trans_N; 1.
DR PIRSF; PIRSF036480; FormyFH4_hydr; 1.
DR PRINTS; PR01575; FFH4HYDRLASE.
DR SUPFAM; SSF55021; ACT-like; 1.
DR SUPFAM; SSF53328; Formyltransferase; 1.
DR PROSITE; PS51671; ACT; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01927};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01927}; Purine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01927}.
FT DOMAIN 15..101
FT /note="ACT"
FT /evidence="ECO:0000259|PROSITE:PS51671"
FT ACT_SITE 234
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01927"
SQ SEQUENCE 290 AA; 31902 MW; 1DC6F45D9BE1DFC0 CRC64;
MAHSTAQPDS AAHLVLSLSC PDRPGIVHAV TGTLARRGGN ITESKQFGDS STGLFFMRVQ
VLTTVPRVEL EKDLAELAGE YEMEWSLDEV GRAMRTLIMV SKEGHCLTDL LFRARSQGLP
VDVVGVVGNH ETLRDVAEFY GVPFHHIPVT KETKEAAEAE LLGLVDSLDV ELVVLARYMQ
ILSPTLCERL HGGVINIHHS FLPSFKGARP YAQAHERGVK LIGATAHYVT ADLDEGPIIE
QDVTRAGHED SVSMLQAKGQ DVERRVLAQA VRWHTEHRVL LNGHRTVVFA
//
