ID E8QWS6_ISOPI Unreviewed; 710 AA.
AC E8QWS6;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE SubName: Full=Isoamylase {ECO:0000313|EMBL:ADV62976.1};
GN OrderedLocusNames=Isop_2400 {ECO:0000313|EMBL:ADV62976.1};
OS Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B).
OC Bacteria; Planctomycetota; Planctomycetia; Isosphaerales; Isosphaeraceae;
OC Isosphaera.
OX NCBI_TaxID=575540 {ECO:0000313|EMBL:ADV62976.1, ECO:0000313|Proteomes:UP000008631};
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 43644;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Saunders E., Brettin T.,
RA Detter J.C., Han C., Tapia R., Land M., Hauser L., Markowitz V.,
RA Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Eisen J.A.;
RT "The complete sequence of chromosome of Isophaera pallida ATCC 43644.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADV62976.1, ECO:0000313|Proteomes:UP000008631}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43644 / DSM 9630 / IS1B
RC {ECO:0000313|Proteomes:UP000008631};
RX PubMed=21475588; DOI=10.4056/sigs.1533840;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Goker M., Cleland D., Saunders E., Lapidus A., Nolan M., Lucas S.,
RA Hammon N., Deshpande S., Cheng J.F., Tapia R., Han C., Goodwin L.,
RA Pitluck S., Liolios K., Pagani I., Ivanova N., Mavromatis K., Pati A.,
RA Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA Detter J.C., Beck B., Woyke T., Bristow J., Eisen J.A., Markowitz V.,
RA Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Isosphaera pallida type strain (IS1B).";
RL Stand. Genomic Sci. 4:63-71(2011).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; CP002353; ADV62976.1; -; Genomic_DNA.
DR RefSeq; WP_013565264.1; NC_014962.1.
DR AlphaFoldDB; E8QWS6; -.
DR STRING; 575540.Isop_2400; -.
DR KEGG; ipa:Isop_2400; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_011725_1_1_0; -.
DR InParanoid; E8QWS6; -.
DR OrthoDB; 226102at2; -.
DR Proteomes; UP000008631; Chromosome.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000008631}.
FT DOMAIN 164..574
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 472..500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 710 AA; 80286 MW; 24F24B391BDBBFD4 CRC64;
MRVWPGVPYP LGANWDGTGV NFALFSENAT NVELCLFDSA DSPKETCRIP LMEQTDRVWH
CYLPDAKPGQ VYGYRVYGPY DPSQGHRFNP AKVVLDPYAK GIARDPKWDD ALFGYRVGDE
QADLSQDDRD SAAFAPLAEV IDPAFDWGGD RKPNHPWHET VIYELHVKGF SKCKPDVPEP
LRGTYAGLAS EGSIQYLKDL GVTAVELLPI HAFLDDRHLI ENGLCNYWGY NTLGFFAPSR
KYSTAQTASD SVREFKELVK GLHAAGLEVI LDVVYNHTAE GNHLGPTLSF RGIDNASYYR
LVPDDRRYYM DFTGCGNTLN MLSPHVLQLI MDSLRYWVLE MRVDGFRFDL ASALARELHE
VDKLGAFFDI IHQDPVLSQV KLIAEPWDLG PGGYQVGNFP IGWTEWNGKY RDCVRKFWKG
DGGTLSEFAS RLCGSGDLYE HSGRRPYASI NFVTCHDGFT LRDLVSYNHK HNEANGEENR
DGADHNESWN CGHEGPTDDP EINQLRARQQ RNFLATLMLS QGVPMLLAGD EIGHTQQGNN
NTYCQDNQLS WLNWDLNEEQ KTLLKFVQSL TQLHRTQAVL QRRTFFQGRT IRGSEVEDVA
WFDPSGREMS DDAWDAGFVK CFGVRWAGDA IGEVDRQGKR IQGDTLLLLF NAHHEPIPFT
LPAKRPDQTW ERLLDTVHPL VEPQPIADDQ PYDLQARSLA VLRIQATPQA
//
