ID E9AE04_LEIMA Unreviewed; 2662 AA.
AC E9AE04;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 24-JAN-2024, entry version 81.
DE RecName: Full=1-phosphatidylinositol 4-kinase {ECO:0000256|ARBA:ARBA00012169};
DE EC=2.7.1.67 {ECO:0000256|ARBA:ARBA00012169};
GN ORFNames=LMJF_29_1450 {ECO:0000313|EMBL:CBZ12483.1};
OS Leishmania major.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX NCBI_TaxID=5664 {ECO:0000313|EMBL:CBZ12483.1, ECO:0000313|Proteomes:UP000000542};
RN [1] {ECO:0000313|EMBL:CBZ12483.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=16020728; DOI=10.1126/science.1112680;
RA Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G.,
RA Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A.,
RA Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M.,
RA Bianchettin G., Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E.,
RA Ciarloni L., Clayton C., Coulson R.M., Cronin A., Cruz A.K., Davies R.M.,
RA De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N.,
RA Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A.,
RA Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S.,
RA Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M.,
RA Masuy D., Matthews K., Michaeli S., Mottram J.C., Muller-Auer S.,
RA Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M.,
RA Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E.,
RA Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C.,
RA Rutter S., Saunders D., Schafer M., Schein J., Schwartz D.C., Seeger K.,
RA Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V.,
RA Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J.,
RA Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D.,
RA Barrell B., Myler P.J.;
RT "The genome of the kinetoplastid parasite, Leishmania major.";
RL Science 309:436-442(2005).
RN [2] {ECO:0000313|EMBL:CBZ12483.1, ECO:0000313|Proteomes:UP000000542}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/IL/81/Friedlin {ECO:0000313|Proteomes:UP000000542};
RX PubMed=22038252; DOI=10.1101/gr.122945.111;
RA Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA Hertz-Fowler C., Mottram J.C.;
RT "Chromosome and gene copy number variation allow major structural change
RT between species and strains of Leishmania.";
RL Genome Res. 21:2129-2142(2011).
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. Type III PI4K
CC subfamily. {ECO:0000256|ARBA:ARBA00006209}.
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DR EMBL; FR796425; CBZ12483.1; -; Genomic_DNA.
DR RefSeq; XP_003722225.1; XM_003722177.1.
DR STRING; 5664.E9AE04; -.
DR EnsemblProtists; CBZ12483; CBZ12483; LMJF_29_1450.
DR GeneID; 12981006; -.
DR KEGG; lma:LMJF_29_1450; -.
DR VEuPathDB; TriTrypDB:LmjF.29.1450; -.
DR VEuPathDB; TriTrypDB:LMJFC_290022700; -.
DR VEuPathDB; TriTrypDB:LMJLV39_290021300; -.
DR VEuPathDB; TriTrypDB:LMJSD75_290021800; -.
DR eggNOG; KOG0902; Eukaryota.
DR HOGENOM; CLU_230048_0_0_1; -.
DR InParanoid; E9AE04; -.
DR OMA; VKACIFK; -.
DR Proteomes; UP000000542; Chromosome 29.
DR GO; GO:0020016; C:ciliary pocket; ISO:GeneDB.
DR GO; GO:0005737; C:cytoplasm; ISO:GeneDB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004430; F:1-phosphatidylinositol 4-kinase activity; IBA:GO_Central.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05167; PI4Kc_III_alpha; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 1.25.40.70; Phosphatidylinositol 3-kinase, accessory domain (PIK); 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR001263; PI3K_accessory_dom.
DR InterPro; IPR042236; PI3K_accessory_sf.
DR InterPro; IPR045495; PI4K_N.
DR InterPro; IPR015433; PI_Kinase.
DR PANTHER; PTHR10048:SF15; PHOSPHATIDYLINOSITOL 4-KINASE ALPHA; 1.
DR PANTHER; PTHR10048; PHOSPHATIDYLINOSITOL KINASE; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF00613; PI3Ka; 1.
DR Pfam; PF19274; PI4K_N; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
DR PROSITE; PS51545; PIK_HELICAL; 1.
PE 3: Inferred from homology;
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Reference proteome {ECO:0000313|Proteomes:UP000000542};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CBZ12483.1}.
FT DOMAIN 2074..2256
FT /note="PIK helical"
FT /evidence="ECO:0000259|PROSITE:PS51545"
FT DOMAIN 2360..2646
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT REGION 60..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 867..907
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 613..630
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 871..896
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2662 AA; 290632 MW; 476E4F94D257CB28 CRC64;
MNDIRLPCWI KSSPTLVMAS ALAQLLTDEL AETTSSATAG APRPPSEVCV LGGRERWPRH
NFDEDISSSS SDDGGGVNLS DSAASECEGC YDSGRDAMRT GGGVGTGYLR MVDRLLELNS
KELDSLPPPG ATATAATYSP PKAVTAPATP MPTAIAGSWH RGSAVLFTGQ NGGASMSTLG
ASPDILRDPR VRTLLRLSRL NDVLTRAETA QRLISRRTAA AMVSFFAFAV SCRYSGLPVD
RTKGFFTECS ALYTTLLSKL RSLRFGGGVN LEDFTASLVA CGLKLRDDED VEGDEKDGRG
GTNEAGATGA VTAGISCSGR SNSSSAVASG AAAMGSCGGA GVRTPMQLSF GRDKNWGLVG
FGHRALDAIF VRCIPSNIDE CVHDPVALGV LRGISLTHSQ QLFYLGPELV NHLHDLTDAF
ATSGHRRALP YKRVLSVLLE AISCSRVIDY ERTNNWITAV IQERYVVRGL FQREELHAHI
VTATLTMLIR QYQEMAKECA SKVVDLGSMP DFTEGDQLIE LVWKALQDTI GSWEDAVATA
EKHPLTLFRH NIDIVVQHLL RTEVERLRCY YIITSATAAY HQSLVGMSAG TRTTGNQSGC
PPAVPQLAPG REEQRGANRR GRDTLRGQDG STRRHSGAPA AAPYVPSAEE ESLRISICSN
IIKLLLLPTS IMSALPRTEE AKQLRASLTE AAARVLSVVT AKEFNSRDDT SVMTTASGRA
ATSAAPRSVS PISAAAATPR IRHADAGAAG TDLLAISRRQ AHFSNNFYRC VEELIHELIH
TILQQSVPDD ATAVANRSVL GGVSDARLST PSTSDTNSDA ILRGMVAAVC IQYMCFTNQH
LRSFAISEVM DAVVRLSARC GAEEAATATH GSLERKSSSK TARASGSDSN ESPAAAPQGT
VVGAAAAAAD PEEKRKLATH VRARQLALCF DALSAILVPT VVVEGYHHVA EEQEERAGLA
RSCTTTSARG NSKREELSRY GQLLCDTYVD SLREHLWGLG EGSVSHHKML TPVMAVAVKA
FVNLVLRVHE RLRGMSSMAL ALVQEAEAAM EGSKKGTQRS AAAIARDRAR LLHAFNAERM
SVVLLLRGVI QNMLSVAWPS SGVLYQDRAC SWERPLNNKT ASAVMAPIAL AVLSPFFRPL
AMLLHLSTLF PARSLRQLCT HYQYARYLTL EDPSTSDQYV FYYYRRLSRA LVAVEVDDSG
GSGAGSAEQI EFECWALFRS CWMMFSYYKY TAEALMAVRA GGMAPMESAN PATATAALAR
APILSTKQCK LLCLIAASAA PLLRMCSSDV QQAMADIAVL LRYTLAGALH CPMVVADVTA
KSGIVHASLY KSLKRLCGGR KECWKRLSTG ELMLLQCVAE LEILRAAAGS VAQLTLYRHF
EVREFVASAA IPEALGHILS TACDHYIEAV RSMLPGVAFQ VTRTDLMQLV FLVSFAIGSV
RESASKLLLC VVRSFPTYAA YASALPLLWC MLDLLEAGTA WQIESLCEHV RFPAVPAVAA
DPHSVERQQH VLFVADVAKR WAAILQDEAP IALFETAIKF MVVQQQECGE QATTQAGRHH
AGSRLAALAS QYRVEEAPPN LDDSAITVMR GAGAAGHVVK TEYAQTLLLH GRAQGAMQAS
SWYASAEQMH STVVAQLWAA TRLCRRDLAS AVAQRLESNL LRPARALFVE TGYSVGTVLA
PRAFTVADED AAAREEVGVT SVPLGAPARA ETAASFAAAV ALLAKGSGTP IVRRQLLQYL
VQGAVQTFRS DVLYEAILCW KWLLSQNREA YLLPMLQELD TAFVWTAANR LGLFDGYCSS
KNRQVQTGEV PIPHNTVSAR GQKGAGVPEE AAVYVADNVD SASPHKLLFS FLTDMYVEEG
SPLCLSPEVL RQLYLVAAHI MQFAKVLSLR DDMFGETMRC FLVVGSIAQL LREANVRQLR
AGLLTIVPFP AIGALRERWY KALLRWFTKM PPSWYYAKDP VLAREEAKVV SSLSQLLKEE
ADLLTRTTLG FLDFSSGVQR ERGMPLVHHW ESLSVVRESV AAVRQAADGG EAGLRKLIAR
EKLRLQGLLH LLRVLVEHEA LRLSVWRTPR RTLKIPNTTP SVGWAKLIEC ADHHNPAVVV
SMVYRFSSIP AVRLAASQRV VAHPERYSNV AEAVDLYLTE DVLRAGAPRL FLFCSCTIIQ
ALRLLDPRYA AYKAVNSYAI CSLLSQRSEN LIFYLPQLLQ LLATDESGSI EAFLVRMSAK
STMFAHQLLW SLQTESEGSG ALAKKCQQVE RRVKAVFTPN EEAFYKSEFA FVDLMTSVSG
EIMKFDKTER KAQLRLRLKD DIFHALPKLR HLYLPTDPNY RITDVIPHTA GAMQSAAKCP
ILVQFTCVPR TAEDTLTPDD VDSAAADEHG HGAMNSHGSA VSRQVVKACI FKMGDDCRQD
QISLQLIGLM QRILNSIGVP SFLYPYRVIT TGKSSGVIEC VPRSRSRNEI GKLVESNVAE
FFVQTFGHPE SAGFRRAREN FVRSTAAYSV VSFILNIKDR HNGNIMIDAD GNLVHIDFGF
LFDTSPGGDI NFESSPFKLT TEMVQLIGMD VSGESTLQSK TLARALVDEE NYIYFKTLVN
RCYLAVRQYA REICVMVELM LRSGLPCFKP KKTIADLAQR LAVDKNEIEA ADYMRRLIHE
SRQNFRTVLY DYYQKVAEGI EM
//
