ID E9EU88_METRA Unreviewed; 1442 AA.
AC E9EU88;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 24-JAN-2024, entry version 51.
DE RecName: Full=Pre-mRNA-splicing factor {ECO:0000256|PIRNR:PIRNR038901};
GN ORFNames=MAA_03587 {ECO:0000313|EMBL:EFZ00991.1};
OS Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS anisopliae (strain ARSEF 23)).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=655844 {ECO:0000313|EMBL:EFZ00991.1, ECO:0000313|Proteomes:UP000002498};
RN [1] {ECO:0000313|EMBL:EFZ00991.1, ECO:0000313|Proteomes:UP000002498}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 23 / ATCC MYA-3075 {ECO:0000313|Proteomes:UP000002498};
RX PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA Gao Q., Jin K., Ying S.H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA Xie X.Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA Zhong Y., Ma L.J., St Leger R.J., Zhao G.P., Pei Y., Feng M.G., Xia Y.,
RA Wang C.;
RT "Genome sequencing and comparative transcriptomics of the model
RT entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL PLoS Genet. 7:E1001264-E1001264(2011).
RN [2] {ECO:0000313|EMBL:EFZ00991.1, ECO:0000313|Proteomes:UP000002498}
RP GENOME REANNOTATION.
RC STRAIN=ARSEF 23 / ATCC MYA-3075 {ECO:0000313|Proteomes:UP000002498};
RX PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA St Leger R.J., Wang C.;
RT "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT adaptation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
CC -!- FUNCTION: Involved in mRNA splicing where it associates with cdc5 and
CC the other cwf proteins as part of the spliceosome.
CC {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome.
CC {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- SIMILARITY: Belongs to the CWF11 family.
CC {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFZ00991.1}.
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DR EMBL; ADNJ02000003; EFZ00991.1; -; Genomic_DNA.
DR RefSeq; XP_007819776.1; XM_007821585.1.
DR GeneID; 19257873; -.
DR KEGG; maj:MAA_03587; -.
DR HOGENOM; CLU_001195_0_0_1; -.
DR OrthoDB; 7041at2759; -.
DR Proteomes; UP000002498; Unassembled WGS sequence.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:InterPro.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:UniProtKB-UniRule.
DR CDD; cd17935; EEXXQc_AQR; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR048966; Aquarius_b-barrel.
DR InterPro; IPR048967; Aquarius_insert.
DR InterPro; IPR032174; Aquarius_N.
DR InterPro; IPR026300; CWF11_fam.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF5; RNA HELICASE AQUARIUS; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF16399; Aquarius_N_1st; 1.
DR Pfam; PF21143; Aquarius_N_2nd; 1.
DR Pfam; PF21144; Aquarius_N_3rd; 1.
DR PIRSF; PIRSF038901; AQR_cwf11; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW mRNA processing {ECO:0000256|PIRNR:PIRNR038901};
KW mRNA splicing {ECO:0000256|PIRNR:PIRNR038901};
KW Nucleus {ECO:0000256|PIRNR:PIRNR038901}.
FT DOMAIN 41..429
FT /note="RNA helicase aquarius N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16399"
FT DOMAIN 508..670
FT /note="RNA helicase aquarius beta-barrel"
FT /evidence="ECO:0000259|Pfam:PF21143"
FT DOMAIN 718..809
FT /note="RNA helicase aquarius insertion"
FT /evidence="ECO:0000259|Pfam:PF21144"
FT DOMAIN 824..1120
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1129..1321
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 769..788
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1394..1442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1407..1432
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1442 AA; 163751 MW; 8BCF3FE6A56B8045 CRC64;
MPNAKRLKGS GAAKAGKARE PAIRVQDERP TPAEVEEEEH QFVQLARSHW LKPGKRTVKS
KVKNDVLKQG IWDVLEQDGF QYKSLLLLES LQTLESYLWP GYTDESSNFH VLLIVLIANV
KRREHLETWS LLEDRPDHFS SMFRRVLSLL LDRTLSTSIR THLLGFLIYA FQSLDCSIVR
KECAPLVSIG IWQNLSSETQ RETLLDQNTH LRKAWRASQK RYDAADGATK ARLRFERSWL
YSLLLDFLGL LYDDATKSDD ALYCEKFTEF ITDLQSQLPT RRYVNTLLQD LHVLPVMKLS
PTYNDENNSL LRDLHSLLLH YTHFTIDDQT GIQLNMTEAY DKHCATLGKL QQVALKHFKE
KLMVLALSNY GGIEKRDELA SLLAPLTDEE LTQLFTLLDM RSTYPESLAL PVDRKLLTEI
MLSKFERKKT FRETSRYMPL VPTERTLFDS GFQRADAYDG SHPLALPKLN LQYLSAGDFL
WRAMILYRCE AFYGIRKDVE SALRRLRPES KQPGVTTFAG FSKMALPISK PSILEVVPPL
VGEELPSMVK AEISFDVRRL GQNVRREWDS LRPDDVVFLL AVEAPSSQAP ANGDDIRSEH
EKLGVISVRS AEIISITDEK GRHVRDGSLN LDNKRRMHLK LDSYMYSRDS ERASAGKPDV
YGQINLLLRR GRRENNFKPV LESIRKLILS EMPLPPWLHE VFLGYGDPAG ANYKNLSNRN
KKLDYRDTFL DWHHLIESLP GKTVEPGGDV TSSFGPPYVL EEAGKVEAAD GVRPSKKRRR
DAEPTMKSEV DTLKVSTYKP PNNGPYPVDV PKMNSVRFTP AQIEAIMSGS QPGLTVIVGP
PGTGKTDVAT QIINNIYHNF PQEKTLLIAH SNQALNQLFA KIVALDIDER HLLRLGHGEE
ELDTDGSFSK HGRVESFLDN RQTFLQEVRK LASSLGAPGA HDNSAETAGY FNKVYVEPAW
KRFQHAATSG ETSVPDLVKA FPFHAYFADA PQPLFPGDAD RETTLDIANG CYRHISKIFS
ELADIVPFEI LRRDREKANY LLTNEARVIA MTTTHAAIRR GEIASLGFHY DNVIMEEAAQ
ITEVETFMPL AMQKPKNNQV PLKRVVLCGD HLQNSPVIQS LAFRHYANLE QSLFSRLVRL
GVPTVNLDQQ GRARPSIAKL YQWRYPKLDN LPHVQNQTEF LRGNAGFKFD YQFINVPDYK
GRGESEPTPH FIQNLGEAEY AVAIYQYMRL LGYPADKISI LTTYAGQRAL VKDVLAHRCA
KNPIFGLPKA VATVDKYQGE QNDYIILSLT RTSRVGYLRD VRRMTVAVSR ARLGLYILGR
REVFEACPEL RPAFDLLLER PDKLMLVTGE MWPTERPNLE DNSTVEGEVA MENVEHLGQY
VFEMTNTRLE QLQAQGGESA TTAPETIPEE AEEDEAPATY YDEDENEVEP DILEVREGEN
KN
//
