ID E9FTW0_DAPPU Unreviewed; 936 AA.
AC E9FTW0;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=Ubiquitin carboxyl-terminal hydrolase {ECO:0000256|RuleBase:RU366025};
DE EC=3.4.19.12 {ECO:0000256|RuleBase:RU366025};
GN ORFNames=DAPPUDRAFT_310510 {ECO:0000313|EMBL:EFX89435.1};
OS Daphnia pulex (Water flea).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=6669 {ECO:0000313|EMBL:EFX89435.1, ECO:0000313|Proteomes:UP000000305};
RN [1] {ECO:0000313|EMBL:EFX89435.1, ECO:0000313|Proteomes:UP000000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21292972; DOI=10.1126/science.1197761;
RA Colbourne J.K., Pfrender M.E., Gilbert D., Thomas W.K., Tucker A.,
RA Oakley T.H., Tokishita S., Aerts A., Arnold G.J., Basu M.K., Bauer D.J.,
RA Caceres C.E., Carmel L., Casola C., Choi J.H., Detter J.C., Dong Q.,
RA Dusheyko S., Eads B.D., Frohlich T., Geiler-Samerotte K.A., Gerlach D.,
RA Hatcher P., Jogdeo S., Krijgsveld J., Kriventseva E.V., Kultz D.,
RA Laforsch C., Lindquist E., Lopez J., Manak J.R., Muller J., Pangilinan J.,
RA Patwardhan R.P., Pitluck S., Pritham E.J., Rechtsteiner A., Rho M.,
RA Rogozin I.B., Sakarya O., Salamov A., Schaack S., Shapiro H., Shiga Y.,
RA Skalitzky C., Smith Z., Souvorov A., Sung W., Tang Z., Tsuchiya D., Tu H.,
RA Vos H., Wang M., Wolf Y.I., Yamagata H., Yamada T., Ye Y., Shaw J.R.,
RA Andrews J., Crease T.J., Tang H., Lucas S.M., Robertson H.M., Bork P.,
RA Koonin E.V., Zdobnov E.M., Grigoriev I.V., Lynch M., Boore J.L.;
RT "The ecoresponsive genome of Daphnia pulex.";
RL Science 331:555-561(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|RuleBase:RU366025};
CC -!- SIMILARITY: Belongs to the peptidase C19 family.
CC {ECO:0000256|RuleBase:RU366025}.
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DR EMBL; GL732524; EFX89435.1; -; Genomic_DNA.
DR AlphaFoldDB; E9FTW0; -.
DR STRING; 6669.E9FTW0; -.
DR EnsemblMetazoa; EFX89435; EFX89435; DAPPUDRAFT_310510.
DR KEGG; dpx:DAPPUDRAFT_310510; -.
DR eggNOG; KOG1870; Eukaryota.
DR HOGENOM; CLU_001060_8_1_1; -.
DR InParanoid; E9FTW0; -.
DR OMA; RIWPQRV; -.
DR PhylomeDB; E9FTW0; -.
DR Proteomes; UP000000305; Unassembled WGS sequence.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd06466; p23_CS_SGT1_like; 1.
DR CDD; cd02674; Peptidase_C19R; 1.
DR Gene3D; 2.60.40.790; -; 1.
DR Gene3D; 6.10.140.2220; -; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 2.
DR InterPro; IPR007052; CS_dom.
DR InterPro; IPR008978; HSP20-like_chaperone.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR InterPro; IPR002893; Znf_MYND.
DR PANTHER; PTHR21646; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR PANTHER; PTHR21646:SF74; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 19; 1.
DR Pfam; PF04969; CS; 1.
DR Pfam; PF00443; UCH; 1.
DR Pfam; PF01753; zf-MYND; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF144232; HIT/MYND zinc finger-like; 1.
DR SUPFAM; SSF49764; HSP20-like chaperones; 1.
DR PROSITE; PS51203; CS; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
DR PROSITE; PS50865; ZF_MYND_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU366025};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Protease {ECO:0000256|RuleBase:RU366025};
KW Reference proteome {ECO:0000313|Proteomes:UP000000305};
KW Thiol protease {ECO:0000256|RuleBase:RU366025};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366025};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00134}.
FT DOMAIN 71..173
FT /note="CS"
FT /evidence="ECO:0000259|PROSITE:PS51203"
FT DOMAIN 237..914
FT /note="USP"
FT /evidence="ECO:0000259|PROSITE:PS50235"
FT DOMAIN 545..584
FT /note="MYND-type"
FT /evidence="ECO:0000259|PROSITE:PS50865"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 195..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 630..663
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 720..754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 916..936
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..52
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 723..754
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 936 AA; 105003 MW; CCD6FDDF62F1A33B CRC64;
MVTCSLKGGA GERRPNNSVP SSSGVLDASN HPTGQAEVMC RSDDEEKHNG TNSNSLDDSL
PMELCPTIHP VHNLKLDHHD HPETVSVTLY VKNIAKESLR VDFGETCISV TFRTSDPGFL
KQYPAFGAED DLMQWQIETR GTIVPEKCTF RLKPTNLELK LIKAVTSKWE SLEATVDSSS
AGPSIQKETW ISLAPPKAAK PSPASRKKFE EDASQETNSI SLSPSADSVM SFKAGYTGLG
NLGNTCYMNA VLQALANTTP LKNYFLDKKF QADINRDNPL GFGGSLAIAF AIILRQLWSG
QRDSIEPSHL KSLLANRASQ FSGYAQHDAQ EFMAFLLDGL HEDLNRVKTK PLTTPVESNG
RPDHIVADEA WQVYKMRNDS VIDDLFQGQF KSTLVCPACQ NISVTFDPFL YLALPLPPSK
SCFTVTVYFD GVDESVELTG TKRGAVKVSL WLDPSQRVQQ LKEELKRVCA GRFRHPDSEI
ELVQVRDGLI RRWLVDAAPL SSLQNTNHSD ALLLAFETAP SDNGSLVHIA VIQRVLMPLD
EHTHCTACKK ESDVLKRCMK CYSVAYCDKN CQRTHWTAVH RTMCRPKPDI IAQPFVVSLP
PSSLTYQKLY RSMLYYSRFS VEVDCPPSAN NSCNQDMDSS SSSSSSSSSP QEEGQVDGLQ
QSKQEQPKEY PLFFIKPIDS QGQGIEKKKR LEDLGDKPLD FDADESPIFL SLDWRNDERS
PNHCSVTVKS PTDYEDLSQE NSDPSADGDT SPNQRRLTLH HCLQQFMQSE VLSKEEAWYC
PKCKEHREAT KQMSVWRLPE ILTIQLKRFS FRNLMWRDKI DQKVHFPVEQ LDLSPYLSSI
TEHGAVYDLY AVVNHYGGIL YGHYTSFARC PAATSDKSQK DVVGWRCMDD RQVRDICEES
VVTSAAYLLL YQRRRRSPGP SASPVQLNYS DPEEID
//
