ID E9HD79_DAPPU Unreviewed; 1457 AA.
AC E9HD79;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=DAPPUDRAFT_328199 {ECO:0000313|EMBL:EFX70331.1};
OS Daphnia pulex (Water flea).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=6669 {ECO:0000313|EMBL:EFX70331.1, ECO:0000313|Proteomes:UP000000305};
RN [1] {ECO:0000313|EMBL:EFX70331.1, ECO:0000313|Proteomes:UP000000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21292972; DOI=10.1126/science.1197761;
RA Colbourne J.K., Pfrender M.E., Gilbert D., Thomas W.K., Tucker A.,
RA Oakley T.H., Tokishita S., Aerts A., Arnold G.J., Basu M.K., Bauer D.J.,
RA Caceres C.E., Carmel L., Casola C., Choi J.H., Detter J.C., Dong Q.,
RA Dusheyko S., Eads B.D., Frohlich T., Geiler-Samerotte K.A., Gerlach D.,
RA Hatcher P., Jogdeo S., Krijgsveld J., Kriventseva E.V., Kultz D.,
RA Laforsch C., Lindquist E., Lopez J., Manak J.R., Muller J., Pangilinan J.,
RA Patwardhan R.P., Pitluck S., Pritham E.J., Rechtsteiner A., Rho M.,
RA Rogozin I.B., Sakarya O., Salamov A., Schaack S., Shapiro H., Shiga Y.,
RA Skalitzky C., Smith Z., Souvorov A., Sung W., Tang Z., Tsuchiya D., Tu H.,
RA Vos H., Wang M., Wolf Y.I., Yamagata H., Yamada T., Ye Y., Shaw J.R.,
RA Andrews J., Crease T.J., Tang H., Lucas S.M., Robertson H.M., Bork P.,
RA Koonin E.V., Zdobnov E.M., Grigoriev I.V., Lynch M., Boore J.L.;
RT "The ecoresponsive genome of Daphnia pulex.";
RL Science 331:555-561(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; GL732622; EFX70331.1; -; Genomic_DNA.
DR STRING; 6669.E9HD79; -.
DR EnsemblMetazoa; EFX70331; EFX70331; DAPPUDRAFT_328199.
DR KEGG; dpx:DAPPUDRAFT_328199; -.
DR eggNOG; KOG0260; Eukaryota.
DR HOGENOM; CLU_000487_3_0_1; -.
DR InParanoid; E9HD79; -.
DR OMA; QAFPIPH; -.
DR PhylomeDB; E9HD79; -.
DR Proteomes; UP000000305; Unassembled WGS sequence.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:GOC.
DR CDD; cd02584; RNAP_II_Rpb1_C; 1.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Reference proteome {ECO:0000313|Proteomes:UP000000305};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 228..524
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
SQ SEQUENCE 1457 AA; 164254 MW; 21EB95EC700A147B CRC64;
MYSVGSETQV TQPTIRQVKR IQLGILSPKE IRKMSVTEGG IKYPEIYEGG KPKLGGLMDP
RQGAIHQSAS CQTCDRNMNE CPGHFGHIDL AKPIFHIGFL MKTIKILRCV CFYCSKLLVS
PSNPKIKKIL VISKGQPRTR MALVHDLCKG INICEIDIET TGHVGCGRYQ PKIRRQGLEL
IGEWKHVDED WQETNLVLTA GRVWEIFKHI SDEDCLILGM DPKYARPDWM IITCLPVSPP
AVRPAVVMHG SASKQNDLTR KLADVVKANE ELLRNVQSGA AAHIIAENIE LLQFHVATMM
INDLEGLPVA QLESGRPIVS IKARLEGRDG RIRGNLMGKR VDFSARTVIT PDPNLRIDQV
GVPRSIAQNI TFPEIVTSFN IEKMQELVQQ GGAKYLTREN GNRFDIRTVK EYPVLKLGDK
VERSIRDGDL VVCNRQPSVQ KVAMTGNRVK VLPWASFRLN PCCTYPFNKY LYSDEMNIHV
PQSMETRAEV ENILLTPRQI ITPQANKPVM GIVQDTLVGT YKLTKRDVFL EKEQMMNLLM
FLPTWDGKMP KPAILKPRPL WTGKQLFTLI IPGNVNMMIT HSTHPDDEDE GPYKWISPGD
TKVMIENGEL VMGILCKKTL GASAASLLHI IFMELGHEVC GRFYGNIQTV INNWLLYEGH
SIGIGDMLVD RQTYMDIQAT IEKAKEDVIE VIQKSRNDEL EPTPGNTLQQ TLENQVNRIL
IDARNETGGS AKNSLTEFNN LKAMVVAGSS GSNVNISQVI ACVGQQNVEG KRIPFGFRKR
TLPHFIKDDF GPESRGFVEN SYVSGLTPSE FYFHAMDRKA LIDTAVKTAN TGYIQRSLIK
AMESVMVHYD GTVRNSAGRI LQFSYGGDGL SAESVELQKM PTVNLSNEAF EKKFKFDPSN
ENYVRRIFNE EITRELISSA EVITEIEHEW EQLYKDREAL RQIFPTGENK VVLPCNLQRM
IWDVQKIFHI NKRAPTDLSP LRVIQGVRDL LAKCVIVVGE DKLSIQANQN ATLLFQCLVR
STLCSKRIAE KYRLSSEAFE WLIGKIETRF QQAQAQPGEM VGALAAQSLG ERPTKMTVNT
FHFAGVSSKN VTLGVARLKE IINISKSPKT PSMTVFLKGQ AARDAEKAKN VLCRLEHTTL
RMVTKNTAIY YDPDPLNTVI LEDQEFVNVY YQRPHFDPTR ISISPWLLRI ELDRKRMTDK
RLTMKQIAEK VNAEFGNSLK IIFSNDSAEK LVLRIRIIMD SDDSSDEEEQ VAKMEDDIFL
RCFEANMLSD MTLQGMESIE KVSMYLPKTD EKKRIVVTET GEFEAIAEWI LETDGTQLMR
VLSERDVDPV RTYSNDIREI FSVLGVEAAR KALEKEMNNL LQFYGLNVNH RHLALLCDVM
TATGQLMPIS SIDRQDTSAL MKCTVKETVS VLLEAAGHAE IDPLRGVSVN LILGQLPRMG
TGCFDLILDA ENIVKFL
//
