ID E9KZL8_VIGRR Unreviewed; 495 AA.
AC E9KZL8; A0A0U4YXN9; A0A1S4ETW2;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4 {ECO:0000256|RuleBase:RU003297};
DE EC=7.1.1.2 {ECO:0000256|RuleBase:RU003297};
GN Name=nad4 {ECO:0000313|EMBL:ADO19877.1,
GN ECO:0000313|RefSeq:YP_004222811.1};
GN ORFNames=ViraM_p08 {ECO:0000313|RefSeq:YP_004222811.1};
OS Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OG Mitochondrion {ECO:0000313|EMBL:ADO19877.1,
OG ECO:0000313|RefSeq:YP_004222811.1}.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3916 {ECO:0000313|EMBL:ADO19877.1};
RN [1] {ECO:0000313|EMBL:ADO19877.1, ECO:0000313|RefSeq:YP_004222811.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21283772; DOI=10.1371/journal.pone.0016404;
RA Alverson A.J., Zhuo S., Rice D.W., Sloan D.B., Palmer J.D.;
RT "The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of
RT Recombination across Short Mitochondrial Repeats.";
RL PLoS ONE 6:E16404-E16404(2011).
RN [2] {ECO:0000313|RefSeq:YP_004222811.1}
RP NUCLEOTIDE SEQUENCE.
RG NCBI Genome Project;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:BAT21322.1}
RP NUCLEOTIDE SEQUENCE.
RA Chooi Y.-H.;
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:BAT21322.1}
RP NUCLEOTIDE SEQUENCE.
RA Lin C.-P., Lo H.-F., Chen C.-Y., Chen L.-F.O.;
RT "The complete mitochondrial genome of mungbean Vigna radiata var. radiata
RT NM92 and a phylogenetic analysis of crops in angiosperms.";
RL Mitochondrial DNA 27:3731-3732(2016).
RN [5] {ECO:0000313|RefSeq:YP_004222811.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000256|RuleBase:RU003297}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000256|RuleBase:RU003297};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Mitochondrion
CC membrane {ECO:0000256|RuleBase:RU003297}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU003297}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family.
CC {ECO:0000256|ARBA:ARBA00009025, ECO:0000256|RuleBase:RU003297}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; HM367685; ADO19877.1; -; Genomic_DNA.
DR EMBL; AP014716; BAT21322.1; -; Genomic_DNA.
DR RefSeq; YP_004222811.1; NC_015121.1.
DR STRING; 3916.E9KZL8; -.
DR GeneID; 10215510; -.
DR OrthoDB; 1946151at2759; -.
DR Proteomes; UP000087766; Mitochondrion MT.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IEA:UniProt.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR NCBIfam; TIGR01972; NDH_I_M; 1.
DR PANTHER; PTHR43507; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4; 1.
DR PANTHER; PTHR43507:SF20; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
PE 3: Inferred from homology;
KW Electron transport {ECO:0000256|RuleBase:RU003297};
KW Membrane {ECO:0000256|RuleBase:RU003297};
KW Mitochondrion {ECO:0000256|RuleBase:RU003297, ECO:0000313|EMBL:ADO19877.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU003297};
KW Reference proteome {ECO:0000313|Proteomes:UP000087766};
KW Respiratory chain {ECO:0000256|RuleBase:RU003297};
KW Translocase {ECO:0000256|ARBA:ARBA00022967};
KW Transmembrane {ECO:0000256|RuleBase:RU003297};
KW Transmembrane helix {ECO:0000256|RuleBase:RU003297};
KW Transport {ECO:0000256|RuleBase:RU003297};
KW Ubiquinone {ECO:0000256|RuleBase:RU003297}.
FT TRANSMEM 12..29
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 36..54
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 89..107
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 143..161
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 173..194
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 214..237
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 307..326
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 338..356
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU003297"
FT DOMAIN 140..419
FT /note="NADH:quinone oxidoreductase/Mrp antiporter membrane
FT subunit"
FT /evidence="ECO:0000259|Pfam:PF00361"
SQ SEQUENCE 495 AA; 54868 MW; B867424D51C7D848 CRC64;
MLEHFCECYS DLSGPILCPV LGSIIPIFIP NSRIRPIRLI GLCASLITFL YSPVPRIQFD
PSTAKSQFVE SLRWLPYENI NLNLGIDGLS FFFVILTTFL IPICILVGWS GMRSYGKEYI
TASLIREFLM IAVFRMLDPL LFYVLPESVL IPMFIIIGVW GSRQRKIKAA YQFFLYTLLG
SVFMLLAILL ILFQTGTADL QISLTTEFSE RRQIFLWIAS FAAFAVKVPM VPVHIWLPEA
HVEAPTAGSV ILAGIPSKLG THGFLRFSIP MFPEATLCST PFIYTPSAIA IIYTSLTTSR
QIDLKKIIAY SSVAHMNLVT IGMFSPNIQG IGGSIPPMLS HGLVPSALFL CVGVLYDRHK
TRLVRYYGGL VSTMPNLSTI SFSSTLANMS SPGTSSFIGE FPISVGAFQR NSLVATLAAL
GMILGAAYSL WLYNRVVSGN LKADFLHKFS DPNGREVSIF IPFLVGVVRM GVHPKVFPDR
MHTYVSNLVQ HGKFN
//
