ID E9PT83_RAT Unreviewed; 2985 AA.
AC E9PT83;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 2.
DT 12-AUG-2020, entry version 65.
DE SubName: Full=Centromere protein F {ECO:0000313|Ensembl:ENSRNOP00000004525};
GN Name=Cenpf {ECO:0000313|Ensembl:ENSRNOP00000004525,
GN ECO:0000313|RGD:628667};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000313|Ensembl:ENSRNOP00000004525, ECO:0000313|Proteomes:UP000002494};
RN [1] {ECO:0000313|Ensembl:ENSRNOP00000004525, ECO:0000313|Proteomes:UP000002494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000004525,
RC ECO:0000313|Proteomes:UP000002494};
RX PubMed=15057822; DOI=10.1038/nature02426;
RG Rat Genome Sequencing Project Consortium;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2] {ECO:0000313|Ensembl:ENSRNOP00000004525}
RP IDENTIFICATION.
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000004525};
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
RN [3] {ECO:0000213|PubMed:22673903}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
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DR EMBL; AABR07022106; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 10116.ENSRNOP00000004525; -.
DR iPTMnet; E9PT83; -.
DR PhosphoSitePlus; E9PT83; -.
DR jPOST; E9PT83; -.
DR PaxDb; E9PT83; -.
DR PRIDE; E9PT83; -.
DR Ensembl; ENSRNOT00000004525; ENSRNOP00000004525; ENSRNOG00000003388.
DR RGD; 628667; Cenpf.
DR eggNOG; ENOG502QVMD; Eukaryota.
DR GeneTree; ENSGT00730000111187; -.
DR HOGENOM; CLU_000551_0_0_1; -.
DR InParanoid; E9PT83; -.
DR OMA; YNAQLVQ; -.
DR TreeFam; TF101133; -.
DR Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR Reactome; R-RNO-68877; Mitotic Prometaphase.
DR Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000003388; Expressed in testis and 19 other tissues.
DR GO; GO:0005930; C:axoneme; ISO:RGD.
DR GO; GO:0005813; C:centrosome; ISO:RGD.
DR GO; GO:0000775; C:chromosome, centromeric region; ISO:RGD.
DR GO; GO:0036064; C:ciliary basal body; ISO:RGD.
DR GO; GO:0097539; C:ciliary transition fiber; ISO:RGD.
DR GO; GO:0000940; C:condensed chromosome outer kinetochore; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0000776; C:kinetochore; ISO:RGD.
DR GO; GO:0030496; C:midbody; ISO:RGD.
DR GO; GO:0005635; C:nuclear envelope; ISO:RGD.
DR GO; GO:0016363; C:nuclear matrix; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0045120; C:pronucleus; ISO:RGD.
DR GO; GO:0005819; C:spindle; ISO:RGD.
DR GO; GO:0000922; C:spindle pole; ISO:RGD.
DR GO; GO:0070840; F:dynein complex binding; ISO:RGD.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0008134; F:transcription factor binding; ISO:RGD.
DR GO; GO:0007059; P:chromosome segregation; ISO:RGD.
DR GO; GO:0001822; P:kidney development; ISO:RGD.
DR GO; GO:0051310; P:metaphase plate congression; ISO:RGD.
DR GO; GO:0000278; P:mitotic cell cycle; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0015031; P:protein transport; ISO:RGD.
DR GO; GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; ISO:RGD.
DR GO; GO:0016202; P:regulation of striated muscle tissue development; ISO:RGD.
DR GO; GO:0021591; P:ventricular system development; ISO:RGD.
DR InterPro; IPR043513; Cenp-F.
DR InterPro; IPR018302; CenpF/LEK1_Rb-prot-bd.
DR InterPro; IPR019513; Centromere_CenpF_leu-rich_rpt.
DR InterPro; IPR018463; Centromere_CenpF_N.
DR PANTHER; PTHR18874; PTHR18874; 2.
DR Pfam; PF10490; CENP-F_C_Rb_bdg; 1.
DR Pfam; PF10473; CENP-F_leu_zip; 3.
DR Pfam; PF10481; CENP-F_N; 1.
PE 1: Evidence at protein level;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Reference proteome {ECO:0000313|Proteomes:UP000002494}.
FT DOMAIN 1..302
FT /note="CENP-F_N"
FT /evidence="ECO:0000259|Pfam:PF10481"
FT DOMAIN 1879..2020
FT /note="CENP-F_leu_zip"
FT /evidence="ECO:0000259|Pfam:PF10473"
FT DOMAIN 2116..2255
FT /note="CENP-F_leu_zip"
FT /evidence="ECO:0000259|Pfam:PF10473"
FT DOMAIN 2298..2437
FT /note="CENP-F_leu_zip"
FT /evidence="ECO:0000259|Pfam:PF10473"
FT DOMAIN 2840..2884
FT /note="CENP-F_C_Rb_bdg"
FT /evidence="ECO:0000259|Pfam:PF10490"
FT REGION 254..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..488
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 761..785
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1105..1136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2767..2917
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2935..2985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 13..131
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 161..188
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 275..323
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 331..405
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 518..645
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 652..700
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 712..753
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 833..860
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 893..927
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 931..951
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 963..997
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1023..1078
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1197..1245
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1294..1321
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1326..1346
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1537..1600
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1612..1632
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1777..1804
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1875..1895
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1945..1982
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2008..2056
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2092..2119
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2127..2241
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2309..2413
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2460..2480
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2488..2522
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2540..2581
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2596..2697
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2707..2734
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2742..2762
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 471..488
FT /note="Polyampholyte"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1105..1124
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2767..2832
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2870..2917
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2938..2952
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2985 AA; 341740 MW; FFB64DF8A9AC2EF2 CRC64;
MSWALEEWKE GLPTRALQKI QELEGQLDKL KKEKQQRQFQ LDSLEAALQK QKQKVEDGKT
EGADLKRENQ RLMEICENLE KTRQKLSHEL QVKESQVNFQ ESQLSSCKKQ IEKLEQELKR
CKSEFERSQQ SAEVSLNPCS TPQKLFTTPL TPSQYYICST YEDLKEKYNK EVEDRKRLEA
EVKALHAKKA SLPVSQATMN HRDIARHQAS SSVFSWQQDK TPSRLSSCSL RTPLRRDVSA
AHFLGEEVTP NKSSVQIGRG DCSGLPDDPH CSQPLHQAKA QNQELKSKMN ELELRLRGQE
KEMKDQVNKF QELQLQLEKT KVDLIEKEKI LNKTRDEVVR TTAQYEQAAA KCTALEQKLK
NLTEELSCHR QNAESAKRSL EQRVKEKEKE LREELSRQHQ SFQALDHEYT QMKTRLTQEL
QQAKHSLSVL QLELEKVTSV KQQLERSLEE IRHKFSRAEQ ALQASQLTEN ELRRSSEEMK
KENSLIRSQS EQRTREACRL EDELGKVKVC LSQSQSFAEE MRAKNTSQEI MLRDLQEKLN
QQENSLTLEK LKLALADLEG QRDCSQDLLK KREHHIEQLN DKLNKIEKEF ETLLSALELK
KKECEELKEE KNQISCWKSE NEKLINQIES EKEILLGKVN HLEASLKTQQ ISHDYSERVR
TLEMERENLT VEIRNLHSLL DSKMVEIETQ KQAYLELQQQ SESSDQKHQK EMENMCLKAN
KLTGQVESLE CKLQSLSGEV ETKDQQYQDL RMEYETMRDS LQARGSSLVT DEKNQRSSSA
FEEQPAVSHS FANLVGEKGS IYSERSDSSV DRGQSPENVA VLQSRVTLLE SSLESQNQMN
SDLQKQCEEL LQIKGEIEEN LIKADQIHQN FVAETNQRIG KLQEDAAVHQ NIVAETLATL
ESREKELQLL KEQLEAQQTE VQKLEKNNCL LEGALKELQL LTDTLSSEKK EMNSIISLRK
KDIEELTQAN GALKEVNEAL RQEKMNLLQQ HEEITRCVAE GERSIAELSG QYKQERLLLL
QRCEETETVL EGLRGDYKAA QENNAKLECM LSECTALCEN KKNELEQLKE TFAKEQQEFL
TKLAFTEEQN RKLVLELETE PQTVRSEITN INKHPVSETD ALGQESWSSK EEQKEKQKEV
SNLTPENEQL MELTQTKHDY YHLEVEPVEN SVKATEEEIR KSSSQYQMDI DTKDISLDSY
KAQLVQLEAL IKVMEVKLDR SEEEKNSLRQ ELQTIREELG TKTSQDTQSQ ARVGLKDCEV
EAEEKYVSVL QELSTSQNEN VHLQCSLQTA MNKLNELGKM CEVLRVEKLQ LESELNDSRS
ECITATSQMA AEVEKLVNEM KMLNHENALS HGELMKDTAD VEFDDKPNHT SVFLTPLDNS
EQMTSSNKEV RVHFAELQEK FSCLQSEHKI LHDQHCEVSS KMSALRSYVD TLKAENSVLS
MNLRTLQGDL VKEKEPGAED GHILPLSFCR TDSPTLTNFG ENSFYKDVLE QTGDTSHLSL
EGNASANPCD VDEVSYSSLE EENLTEKEIP FASLRTVEEL EILCQVYLQS IKNLEEKIES
QRIMKNKEIE ELEQLLSSER KELSCLRKQY LSEKEQWQQK LTSVTLEMES KLAEEKQQTK
NLSLELEVAR LQLQELDLSS RSLLGTDLEG AVRGPNDGYA IKESEVYISE TTEKTPKQDT
NQTCDNDVQQ DLCLETSVTE TETTRLTGDG CEEQPSKISC EAPAEDRTQD YSECISELFS
TPSVLVPMDV LEDQGSIQNL HLQKDTSNEN LRLLPEVEDW DKKVESLLNE IKEADSKLSL
QELQLKIKIA TCIELEKIVK HLKKEETDLS EKLESLPCNQ EVCPRVERSD LDFNLDMGDD
ELFRESTKDD AANTEDNYKE KFLDMERELT RIKSEKANIE HHIVSVKANL EVVQAEKLCL
ERDTESKQKV IVDLKGELFT VISERNRLRE ELDNVSKESK ALDQMSKKMK EKIEELESHQ
RESLHHIGAV ESEVKDKAEL IQTLSFSVDE LTKDKAHLQE QLQNLQNDSQ GLSLAIGELE
IQIGQLNKEK ESLVKESQNF QVKLTESECE KQTISKALEV ALMEKGEFAM RLSSTQEEVH
QLRQGIEKLS VRIEADEKKH LSAVAKLKES QRESDSLKDK VENLERELEM SEENQELAIL
DSENLKAEVE TLKAQMDEMA KSLRVFELDL VNVRSERENL AKQLQEKQSR VSELDELCSS
LRSLSEEKEQ ARVQMERDSK SAMLMLQTQL KELWEEVAAL YNDQETLKAQ EQSLDQPGEE
VHLLKSSIQK LKVHIDADKK KQCHILEQLK ESKHHADLFK DRVENLEQEL MLSEKNKEHL
IFQAENSKAE IQTLKTEIQT MDQNLQDLEL ELTNTRSEKE NLMKELKNEQ EQISKLETIN
SSIERLLKDK EAEXVQVKEE ARITVEMLQT QLKGSNETGG SLCNDQEAYK TKEQNLGSRV
QTLELEKAQL LQDLGEAKNK YIIFQSSVNA LTQEVEAGKQ KLEKEEEEVR TLKEQLKGQE
QLVCKLARVE GEQQLCQKQK LELRSLTMEL EQKVKVLQSE NDALQTTYEA LQSSYKSLEG
ELGLIKMEKM ALVERVNTMT GKEAELQREL HDVEQKSTQL KEEYSKEKTR LTEDLEVVME
ELKNTKVAHL KNVNHLEKEF QRAQGKIKLL LKSCKQLEGE KKMLQKELSQ LEAAQKQRAG
SLVDSNVDEL MTENKELKET LEEKIKEADK YLDKYCSLLI SHEELEKTKE ILEIQVARLS
SRQNKQDLQS SPLLDSSVAG PSPSTSVSER KSTSGLNKTL GKRQRSSGIG ENGNGTAPST
PETFSKKSRK SVSNSAHPAE DEEETEFEPE GLPEVVKKGF ADIPTGKTSP YILRRTTMAT
RTSPRLAAQK LLGSSPTLGK ENLVESSKPT AGGSRSQKVK VVQESSMDAH AVFQELPAKS
LTANNMPGRN STESPREGLR AKRACPASSP AAGPDPMNNE NCRVQ
//
