ID E9Q2E4_MOUSE Unreviewed; 4418 AA.
AC E9Q2E4;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 2.
DT 27-MAR-2024, entry version 93.
DE SubName: Full=HECT domain E3 ubiquitin protein ligase 4 {ECO:0000313|Ensembl:ENSMUSP00000048345.7};
GN Name=Hectd4 {ECO:0000313|Ensembl:ENSMUSP00000048345.7,
GN ECO:0000313|MGI:MGI:3647820};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000048345.7, ECO:0000313|Proteomes:UP000000589};
RN [1] {ECO:0007829|PubMed:16452087}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=16452087; DOI=10.1074/mcp.T500041-MCP200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [2] {ECO:0000313|Ensembl:ENSMUSP00000048345.7, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000048345.7,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0007829|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4] {ECO:0000313|Ensembl:ENSMUSP00000048345.7}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000048345.7};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR RefSeq; NP_852086.3; NM_181421.4.
DR SMR; E9Q2E4; -.
DR STRING; 10090.ENSMUSP00000048345; -.
DR iPTMnet; E9Q2E4; -.
DR PhosphoSitePlus; E9Q2E4; -.
DR SwissPalm; E9Q2E4; -.
DR EPD; E9Q2E4; -.
DR MaxQB; E9Q2E4; -.
DR PaxDb; 10090-ENSMUSP00000048345; -.
DR PeptideAtlas; E9Q2E4; -.
DR ProteomicsDB; 363378; -.
DR Antibodypedia; 31172; 15 antibodies from 7 providers.
DR Ensembl; ENSMUST00000042614.13; ENSMUSP00000048345.7; ENSMUSG00000042744.17.
DR GeneID; 269700; -.
DR KEGG; mmu:269700; -.
DR UCSC; uc008ziv.2; mouse.
DR AGR; MGI:3647820; -.
DR MGI; MGI:3647820; Hectd4.
DR VEuPathDB; HostDB:ENSMUSG00000042744; -.
DR eggNOG; KOG1426; Eukaryota.
DR GeneTree; ENSGT00910000144226; -.
DR HOGENOM; CLU_000179_1_0_1; -.
DR InParanoid; E9Q2E4; -.
DR OMA; TRACMVL; -.
DR OrthoDB; 2903695at2759; -.
DR PhylomeDB; E9Q2E4; -.
DR TreeFam; TF331621; -.
DR BioGRID-ORCS; 269700; 3 hits in 71 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; E9Q2E4; Protein.
DR Bgee; ENSMUSG00000042744; Expressed in superior cervical ganglion and 229 other cell types or tissues.
DR ExpressionAtlas; E9Q2E4; baseline and differential.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0042593; P:glucose homeostasis; ISO:MGI.
DR GO; GO:0006006; P:glucose metabolic process; ISO:MGI.
DR CDD; cd13735; SPRY_HECT_like; 1.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR043366; HECTD4.
DR InterPro; IPR035781; SPRY_HECTD4.
DR PANTHER; PTHR46435; E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED; 1.
DR PANTHER; PTHR46435:SF1; E3 UBIQUITIN-PROTEIN LIGASE HECTD4-RELATED; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 1: Evidence at protein level;
KW Proteomics identification {ECO:0007829|EPD:E9Q2E4,
KW ECO:0007829|MaxQB:E9Q2E4};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 4060..4418
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1917..1948
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2039..2062
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2642..2668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3279..3342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3439..3460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3750..3826
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1917..1932
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1933..1948
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2039..2053
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3279..3310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3328..3342
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3439..3457
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3750..3767
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3796..3826
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4386
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4418 AA; 484128 MW; CDB6D77DC5C6D46C CRC64;
MGSSAAAAAA AAAAAAAADS AQWLSVKEET IFLHDGLIRV TDLAELPSEI LGAPEAADTD
LEILTFETKN PTELAERLRS VCGNQSNAYA RLLEYRLNAL RGLWNAQRQL ALEEQHEREG
SGDEETLALL KRQGLLQQPE QAPFTSRVGL LLVFPLIQSQ SRTDPSLCNI TAEVLLNCLR
DCQPLSLTKE PADCLNGIET LLCSWLEETS DTGRHIPHKQ KENAAAALVA LACARGSLKT
FVHTVHLLQR QTELGSLPVA DVLYRLLLLE GGPGSPSCLL GGKHIVSWGY EDMLPAPDSN
TGSSSENKDA DLGRCLTADG HYLYTTNSAG RGVSKLGSGL HGTLRGFVYC RNEELEPGWV
AYGNGHLLHR PVSFDNKPHS LFQVIDQNTL QVCQTVPMPA SPLPVGSTMS TVHLSSDGTY
FYWIWSPASL NEKTPKGHSV FMDIFELVVE NGVFVANPLQ ERTILMRKEG ESAKSINEML
LSRLSRYRAS PSATLAALTG STISNTLKED QAANTSCGLP LKMLRKTPMY TCGTYLVMLV
PPPGGSGSSA TRSLFGGTSG LSSLKILASS LVYNISDGQF ASRADLIDAA GSSLGRGALV
PGLGACYDTM NNMLWTCSND YIDQWCNPGN QAFHYVCQRL GVSHIITEPK EEAISTNEVI
NQLLHHVGAM CIHQLNLLAT NPNLPITSVL GKQHPIEARH LSSICDIMEK AMVNGDTCIV
RCILVVFQVV FKFFFSPQTE RNRDIIRRSG LLLWQLLMAP KDQICPEIQK EVCLAISSGL
NILYPGETEI NNLLKLVLTE GERNSGLSQL RDVILTNLAE QLQNNRFGSD EDDHYRLNDE
LLHYILKIVV RESCILITKC QTVSKDDFQK LLSTVPAASS CLRYLMAVQN HLLSNTVLIK
PDENEDSDSS LQGETLKELK TSILALATQI LTGCDEVLEM LQQVTTALIN SDIPDREQRL
KGLEQVTKAT MLGHLLPVLL TSLMHPNLQT LTMADALMPQ LVQLVLYTSQ TALLLKTQCP
AFAEMSCSPC GTSDQKCRLF PDERMLEEKE EPGFLTGLKI PAPWAAGKTV ETVHPVRDNY
KFKETVHIPG ARCLYLRFDT RCSSQYDYDK LVIYAGPNTN SRKVAEYGGN TLGYGSRSVL
GTGWPKDLVK VEGDTVTFSF EMRSGREHNT PDKAMWGFAC TVRAQESSED VSGGLPFLVD
LALGLSVLAC SMLRILYNGP EITKEEEACQ DLLRSKLLQR CQWQVEANGV ISPALTPSPS
PLPLTIEEDR EFTYPADVLV PPVGNYFDLP RIRLPPGIMI KLREISGRAR PQFRPSIKEV
IQPDVMEEMV VSCVIKHLNL VDALQSLINF QYQEEHAEEY DLLCKIMGET FKKLNAMERQ
LQSVAELEQK WQSEAEDAMQ GKLENNMPFF YDYHFNENKM KELELLCSMK EVSFDGNDLE
NMVLSLREKF LQEVNSLAQK PSHPLAKTKT LVRSLMNRAE LLLHVTIAAQ AGLTRSISGT
PAETPACKSA SETKVASHAV RQPVFLRSMS APSDLEMIGN EDLEFTRANQ RRRHVTSHRS
SSFTLLQSLA IEDSRDKPTY SVLLGQLFAF IGTNPDQAVS SSSFLLAAQT RWRRGNTRKQ
ALVHMRELLT AAVRVGGVTH LVGPVTMVLQ GGPRIEELTC GGMVEQVQEA FGETMTSVVS
LCARYPIACA NSIGLLCTIP YTRSEEKCLV RSGLVQLMDR LCSLSSQTES SSSEKQTKKQ
KVATMAWAAF QVLANRCVEW EKEEGGSTEA VHSGLARQVS SLLTNHLARA TECCGNQAAG
NDALQDVLSL LNDLSRSHIG KAILSQPACV SKLLSLLLDQ RPSPKLVLII LQLCRAALPL
MSVEDCGNVE LPPWSYSVPS LNSEQEDPSD PASKIASLLL AKLADYVVPG CQTVLSPTAS
EPDTTLTKTS PKNSLKGDKD PGEESEAVDG KLSIFIHKRE DQSSHEVLQP LLSSSEGRPF
RLGTGANMEK VVKMDRDMTK GGCCEVITEE ASAALRKATK WAQSGLIVSV GPPVESINPE
TVSGLSTGDK KKTAQTSICR ERNSELARTD PVRPFISGHV ANSMAAEVIA LLHSLLMAPE
SNAAQIWTTT AEKVLSRALM YIPQLGKYAE SILENGSSSG RKLAKLQRIA RQAVAALCAL
GGFKETIKIG SEVQVLGRGI SGSIGVVASI NEQEGIATVR FPPVDCRRTS QAADTLTIPL
SRLCVPRSEA LPLHKLSITE KVVQAVQSML LPQEGSLSIH TSLPATGDGS APVMAVVRLL
AEIRTRACLV MAQLLEDSLF CEEFIQQCPA AVEVLNLVAQ ECSAGERLAV VEMQCERLRM
LYRDCARPPP PPLQADRRQP KEITWSPSRV FPPVRACMFS SHLTSVTFLA DPSAGGGLPR
GTFIYATSPL PVQAPSFYWE IEIVSYGDTD DDTGPIVSFG FATEAEKRDG AWTNPVGTCL
FHNNGRAVHY NGSSLLQWKS VRLDVTLSPG DVAGIGWERT EGTPPPPGQP AKGRVYFTYC
GQRLTPYLED VSGGMWPVVH IQKKNTKTRA NFGSRPFAYA EGQAHRNAAD LCTDLAEEIS
ANFEALPFAM ASDSDNDAGT SIASDPGTHG PPCRIAAVAT AQQQYDSDTS CHYKVELSYE
NFITSGPDPH PPPIADDESD DDDDDDIPQE DHYALLVKAW ETKVFPTIRR RFRNEAERKS
GLDQIKGALQ LGMVDIARQT VEFLYEENGG IPRDLYLPTI EDIKDEANKF TIDKVRKGLT
VVTRSPDSSN VASSTVGTAL PKFAIRGMLK TFSLHGVVLD VDSVNELVQV ETYLRSEGVL
VRYWYPIDML ERPPAGYRRT ATNGLVTLDN TNLQIHRELL RCEAALARLY CRMALLNIFA
PKLPHLFTRL FHIPAIRDIT LEHLQLLSNQ LLAPPLPDGT ISSSSILLAQ SLQHCIHSQS
CSATDLFYQG SSQTLREWLS VAITRTLHQG EDSLLELTKQ ICAFLQTAPE QFPSEEFPVS
ESKVSMDVNF PGAAFVVVSC KESQSGFRKD SSLYKAPWAR VLVYGLGHKV KRTGQLNLIE
AACYPRDASP ANTGLAPPPT ADQYPSVVLS TDKVHIKLGV SPPPGAVLVL HSLPLEFPLA
MAFAEQLLSW KSEDSDGKSE DEPDSIPASV LLHVVELLGN FLWTTDMAAC VKELVFHLLA
ELLRTVHTLE QRKHPAGLSS SIALQLNPCL AMLMALQSEL HKLYDEETQS WVSSSACGGS
GAAAVGDQGR FSTYFHALME GCLAVAEVTL PTNISVTASG VTSTTTPNLS DSSSSSSSSP
GQTPQSPSLL SKRKKVKMKR EKASSSNKRQ SSRASDADST VLSIGGSKPE DMLWFHRALT
LLIILRHLTK KDPQGLGVTS DAIADACQAL VGPTAHSRLL VISGIPTHLD EAVVRGAIRK
ACNAHGGVFK DEIYIPLQDE DPKKPKDKAE GSDSKVESEK LLGFPSVDSL EGSTSSSLAP
AMSISASAST SQASVCSSQC VSQTASDLSV DPLPSGLELP VPPVLLEPHV VSSQESLDIS
LCSTGSLGSL GSLGEPLDNP ETASVSDVGS MYTVTSLDTQ PLATRPIKGF AVVEIRSRAK
IEKIRASLFN NNDLIGLSSL DGEDELMEMS TEEILTVSVV NQSLFDTQGS PGLEDYFSDK
SIKGEKLVPG AREVLTEIFK SCAHSEQTLS LTPAKPIRVS DIYLSKEQIN SQTPGNLLHL
FFTNVRPPKK VLEDQLTQIL RKYGVPKPKL DKSKYSKAGK EQHPVKVVST KRPVTKPPAK
DKAVLSSLSR TALSEKKPTV KPKSPEKSKP DEKDPEKSPT KKQEVPEEKY LTLEGFHKFV
IDRAKQDIRS VWRAILSCGY DLHFERCACI DVRHAQKASR KWTLEMDVAL VQYINRLCRH
LAITPARLHP HEVYLDPADA ADPRMACLSN VPIESLRLRF ALLQSLNTTL ETFFLPLVEL
RQTPMYAHSI AALLKEAKGL IFYDTKVTVM NRVLNATVQR TADHAAPEIT LDPLEIVGGE
IRASENSYFC QAARQLASVP SSQLCVKLAS GGDPTYAFNI RFTGEEVHGT SGSFRHFLWQ
VCKELQSSSL SLLLLCPSSA VNKNKGKYIL TPSPITYGEE QLLHFLGQLL GIAIRADVPL
PLDLLPSFWK TLVGEPLDPD QDLQEADILT YNYVKKFESI NDESELEALC AEIASQHLAT
ESPEGPKPCC RFTYLTMTGE EVELCSRGRH IPVAWENKDI YAAAIRSLRL RELQNMECVT
AVRAGLGSII PLQLLTTLSP LEMELRTCGL PYINLEFLKA HTMYQVGLME TDQHIELFWG
ALEMFTQEEL CKFIKFACNQ ERIPFTCPCK DGGPDTAHVP PYPMKIAPPD GTAGPPDSRY
IRVETCMFMI KLPQYSSLET MLEKLRCAIH YREDPLSG
//
