ID E9Q3P4_MOUSE Unreviewed; 2997 AA.
AC E9Q3P4;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 10-FEB-2021, entry version 72.
DE SubName: Full=Centromere protein F {ECO:0000313|Ensembl:ENSMUSP00000129738};
GN Name=Cenpf {ECO:0000313|Ensembl:ENSMUSP00000129738,
GN ECO:0000313|MGI:MGI:1313302};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000129738, ECO:0000313|Proteomes:UP000000589};
RN [1] {ECO:0000313|Ensembl:ENSMUSP00000129738, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000129738,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000213|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3] {ECO:0000313|Ensembl:ENSMUSP00000129738}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000129738};
RG Ensembl;
RL Submitted (MAY-2011) to UniProtKB.
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DR EMBL; AC140679; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001074832.2; NM_001081363.2.
DR SMR; E9Q3P4; -.
DR IntAct; E9Q3P4; 12.
DR STRING; 10090.ENSMUSP00000129738; -.
DR EPD; E9Q3P4; -.
DR jPOST; E9Q3P4; -.
DR MaxQB; E9Q3P4; -.
DR PaxDb; E9Q3P4; -.
DR PeptideAtlas; E9Q3P4; -.
DR PRIDE; E9Q3P4; -.
DR Antibodypedia; 4038; 260 antibodies.
DR Ensembl; ENSMUST00000171929; ENSMUSP00000129738; ENSMUSG00000026605.
DR GeneID; 108000; -.
DR KEGG; mmu:108000; -.
DR UCSC; uc007eao.2; mouse.
DR CTD; 1063; -.
DR MGI; MGI:1313302; Cenpf.
DR eggNOG; ENOG502QVMD; Eukaryota.
DR GeneTree; ENSGT00730000111187; -.
DR HOGENOM; CLU_000551_0_0_1; -.
DR InParanoid; E9Q3P4; -.
DR OrthoDB; 205405at2759; -.
DR TreeFam; TF101133; -.
DR Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR Reactome; R-MMU-68877; Mitotic Prometaphase.
DR Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR BioGRID-ORCS; 108000; 1 hit in 17 CRISPR screens.
DR ChiTaRS; Cenpf; mouse.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; E9Q3P4; protein.
DR Bgee; ENSMUSG00000026605; Expressed in primary oocyte and 221 other tissues.
DR Genevisible; E9Q3P4; MM.
DR GO; GO:0005930; C:axoneme; IDA:MGI.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0000775; C:chromosome, centromeric region; ISO:MGI.
DR GO; GO:0036064; C:ciliary basal body; IDA:MGI.
DR GO; GO:0097539; C:ciliary transition fiber; IDA:MGI.
DR GO; GO:0000940; C:condensed chromosome outer kinetochore; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0016363; C:nuclear matrix; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0045120; C:pronucleus; IDA:MGI.
DR GO; GO:0005819; C:spindle; ISO:MGI.
DR GO; GO:0000922; C:spindle pole; ISO:MGI.
DR GO; GO:0070840; F:dynein complex binding; ISO:MGI.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0008134; F:transcription factor binding; ISO:MGI.
DR GO; GO:0007059; P:chromosome segregation; ISO:MGI.
DR GO; GO:0001822; P:kidney development; ISO:MGI.
DR GO; GO:0051310; P:metaphase plate congression; ISO:MGI.
DR GO; GO:0000278; P:mitotic cell cycle; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0015031; P:protein transport; ISO:MGI.
DR GO; GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; ISO:MGI.
DR GO; GO:0016202; P:regulation of striated muscle tissue development; IDA:MGI.
DR GO; GO:0021591; P:ventricular system development; ISO:MGI.
DR InterPro; IPR043513; Cenp-F.
DR InterPro; IPR018302; CenpF/LEK1_Rb-prot-bd.
DR InterPro; IPR019513; Centromere_CenpF_leu-rich_rpt.
DR InterPro; IPR018463; Centromere_CenpF_N.
DR PANTHER; PTHR18874; PTHR18874; 2.
DR Pfam; PF10490; CENP-F_C_Rb_bdg; 1.
DR Pfam; PF10473; CENP-F_leu_zip; 3.
DR Pfam; PF10481; CENP-F_N; 1.
PE 1: Evidence at protein level;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Proteomics identification {ECO:0000213|EPD:E9Q3P4,
KW ECO:0000213|MaxQB:E9Q3P4, ECO:0000213|PeptideAtlas:E9Q3P4};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589}.
FT DOMAIN 1..300
FT /note="CENP-F_N"
FT /evidence="ECO:0000259|Pfam:PF10481"
FT DOMAIN 1894..2035
FT /note="CENP-F_leu_zip"
FT /evidence="ECO:0000259|Pfam:PF10473"
FT DOMAIN 2131..2270
FT /note="CENP-F_leu_zip"
FT /evidence="ECO:0000259|Pfam:PF10473"
FT DOMAIN 2313..2449
FT /note="CENP-F_leu_zip"
FT /evidence="ECO:0000259|Pfam:PF10473"
FT DOMAIN 2852..2896
FT /note="CENP-F_C_Rb_bdg"
FT /evidence="ECO:0000259|Pfam:PF10490"
FT REGION 206..227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 462..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 763..797
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1522..1548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1711..1737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2774..2997
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 13..131
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 159..186
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 273..335
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 343..395
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 516..612
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 623..643
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 657..677
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 710..737
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 831..879
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 905..939
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 968..1005
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1010..1090
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1095..1115
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1131..1165
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1208..1256
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1269..1289
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1311..1331
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1550..1613
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1625..1645
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1790..1810
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1825..1852
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1883..1910
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1946..1997
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2023..2078
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2107..2127
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2142..2197
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2205..2285
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2324..2351
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2356..2457
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2465..2492
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2500..2534
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2559..2600
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2615..2709
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2719..2746
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2754..2774
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 206..226
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..490
FT /note="Polyampholyte"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2775..2816
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2837..2867
FT /note="Polyampholyte"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2882..2964
FT /note="Polar"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2997 AA; 342472 MW; 3AEE30F1D554DEC5 CRC64;
MSWALEEWKE GLPSRALQKI QELEGQLEKL KKEKQQRQFQ LDSLEAALQK QKQKVEDGKT
EGADLKRENQ RLMEICEHLE KSRQKLSHEL QVKESQVNLQ ESQLSSCKKQ IEKLEQELKR
CKSEFERSQQ VAQSADVSLN PCSTPQKLFA TPLTPSSTYE DLKEKYNKEV EERKRLEEEV
KALHAKKVSL PVSQATMNHR DIARHQASSS VFPWQQENTP SRLSSDALKT PLRRDGSAAH
FLGEEVSPNK SSMKTGRGDC SSLPGEPHSA QLLHQAKAQN QDLKSKMTEL ELRLQGQEKE
MRSQVNKCQD LQLQLEKTKV ELIEKERILN KTRDEVVRST AQYDQAAAKC TTLEQKLKTL
TEELSCHRQN AESAKRSLEQ RIKEKEKELQ EELSRQHQSF QALDSEYTQM KTRLTQELQQ
VKHLHSTLQL ELEKVTSVKQ QLERNLEEIR LKLSRAEQAL QASQVAENEL RRSSEEMKKE
NSLIRSQSEQ RTREVCHLEE ELGKVKVSLS KSQNFAEEMK AKNTSQEIML RDLQEKLNQQ
ENSLTLEKLK LALADLERQR NCSQDLLKKR EHHIDQLNNK LNKIEKEFET LLSALELKKK
ECEELKEEKN QISFWKIDSE KLINQIESEK EILLGKINHL ETSLKTQQVS PDSNERIRTL
EMERENFTVE IKNLQSMLDS KMVEIKTQKQ AYLELQQKSE SSDQKHQKEI ENMCLKANKL
TGQVESLECK LQLLSSEVVT KDQQYQDLRM EYETLRDLLK SRGSSLVTNE DNQRSSEDNQ
RSSEDNQRGS LAFEQQPAVS DSFANVMGRK GSINSERSDC SVDGGRSPEH IAILQNRVTS
LESSLESQNQ MNSDLQMRCE ELLQIKGEVE ENLSKAEQIH QNFVAETNQC ISKLQEDAAV
HQNIVAETLA TLESKEKELQ LLKEKLEAQQ TEVQKLNKNN CLLEGTLKEL QLLSDTLSSE
KKEMNSIISL SKKNIEELTQ ANEALKEVNE ALEQEKMNLL QKHEKITSCI AEQERSIAEL
SDQYKQERLQ LLQRCEETEA VLEDLRGNYK TAQENNAKLE CMLSECTALC ENRKNELEQL
KETFAKEQQE FLTKLAFAEE QNRKLMLELE IEQQTVRSEI TNTNKHSMSA TDGLRQECLT
LNEEQNEQQN EVSNLTHENE QLMELTQTKH DSYLAVEPVE NSVKATEDEI GKSSSQYQMD
IDTKDISLDS YKAQLVHLEA LVRILEVQLD QSEEENKKLH LELQTIREEL ETKSSQDPQS
QARTGLKDCD TAEEKYVSML QELSASQNEN AHLQCSLQTA VNKLNELGKM CDVLRVEKLQ
LESELNDSRT ECITATSQMT AEVEKLVSEM KMLNHESALS QNELMKDTSG GEFHDKANHS
SVFLTPLDSS NFCEQMTLSS KEVRVHFAEL QEKFSCLQSE HKILHDQHCE VSSKMSALRS
YVDTLKAENS ALSMSLRTLQ GDLVKEGEPA AEGGHGLPLS FCGADSPSLT NFGETSFYKD
VLEQTGDTCH LSLEGNASAN SCDLDEEFSS SLEEETLTEK ESPPAPGRTV EGLEVLCQVY
LQSLKNLEEK TESQRIMKNK EIEKLEQLLS SERKELSCLR KQYLSEKEQW QQKLTSVTLE
MESKLAEEKQ QTKTLSLELE VARLQLQELD LSSRSLLGTD LESVVRCQND NYDIKESEVY
ISETTEKTPK QDTDQTCDKD IQQDLGLETS VTESETTRLT GEGCEEQPPK TNCEAPAEDK
TQDCSECISE LCSSSNVLVP MDVLEDQGSI QNLQLQKDTL NENLRLLPEV EDWDKKVESL
LNEIMEADSK LSLQEVQLKM KIATCIQLEK IVKDLRKEKA DLSEKLESLP CNQEVCLRVE
RSEEDLGFNL DMGANELLSK STKDNATNTE DNYKEKFLDM ERELTRIKSE KANIEHHILS
VETNLEVVQA EKLCLERDTE SKQKVIIDLK EELFTVISER NRLREELDNV SKESKALDQM
SKKMKEKIEE LESHQRESLR HIGAVESEVK DKADLIQTLS FNVGELTKDK AHLQEQLQNL
QNDSQELSLA IGELEIQIGQ LNKEKESLVK ESQNFQIKLT ESECEKQTIS KALEVALKEK
GEFAVQLSSA QEEVHQLRRG IEKLSVRIEA DEKKHLSAVA KLKESQRESD SLKDTVETLE
RELERSEENQ ELAILDSENL KAEVETLKAQ KDEMTKSLRI FELDLVTVRT ERENLAKQLQ
EKQSRVSELD ERCSSLRRLL EEKEQARVQM EEDSKSAMLM LQMQLKELRE EVAALCNDQE
TLKAQEQSLD QPGEEVHHLK SSIRKLKVHI DADEKKHQNI LEQLKESKHH ADLLKDRVEN
LEQELILSEK NMIFQAEKSK AEIQTLKSEI QRMAQNLQDL QLELISTRSE NENLMKELKK
EQERVSDLET INSSIENLLK DKEQEKVQMK EEAKITVEML QTQLKELNET VVSLCNDQEV
SKTKEQNLGS QVQTLELEKA QLLQDLGEAK NKYIIFQSSV NALTQEVEAG KQKLEKGEKE
IRTLKEQLKS QEQLVCKLAQ VEGEQQLWQK QKLELRNVTM ALEQKVQVLQ SENNTLQSTY
EALQNSHKSL ESELGLIKLE KVALVERVST ISGKEAELQR ELRDMLQKTT QLSEDYNKEK
NRLTEEVEVL REELQNTKAA HLKSVNQLEK ELQRAQGKIK LMLKSCRQLE GEKEMLQKEL
SQLEAAQQQR AGSLVDSNVD EVMTENKALK ETLEEKVKEA DKYLDKYCSL LISHEELEKA
KEILEIEVAR LKSRQSRQDL QSSPLLNSSI PGPSPNTSVS EMKSASGQNK ASGKRQRSSG
IWEHGKRAAP STAETFSKKS RKSDSKSTRP AEHEQETEFE PEGLPEVVKK GFADIPTGKT
SPYILRRTTM ATRTSPRFAT QKLVGSSPSL GKENVVESSK PTAGGSRSQK VKVVQESSAD
SHTAFQELPA KSLTASNIPG RNSTESPREG LRAKRAYPAS SPAAGPDPTN NENCRVQ
//
