ID EPO_SHEEP Reviewed; 194 AA.
AC P33709; Q28572;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 24-JAN-2024, entry version 108.
DE RecName: Full=Erythropoietin;
DE Flags: Precursor;
GN Name=EPO;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RX PubMed=8349021; DOI=10.1016/0303-7207(93)90113-x;
RA Fu P., Evans B., Lim G.B., Moritz K., Wintour M.E.;
RT "The sheep erythropoietin gene: molecular cloning and effect of hemorrhage
RT on plasma erythropoietin and renal/liver messenger RNA in adult sheep.";
RL Mol. Cell. Endocrinol. 93:107-116(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-194.
RC TISSUE=Kidney;
RX PubMed=8364201;
RA Wen D., Boissel J.-P.R., Tracy T.E., Gruninger R.H., Mulcahy L.S.,
RA Czelusniak J., Goodman M., Bunn H.F.;
RT "Erythropoietin structure-function relationships: high degree of sequence
RT homology among mammals.";
RL Blood 82:1507-1516(1993).
CC -!- FUNCTION: Hormone involved in the regulation of erythrocyte
CC proliferation and differentiation and the maintenance of a
CC physiological level of circulating erythrocyte mass. Binds to EPOR
CC leading to EPOR dimerization and JAK2 activation thereby activating
CC specific downstream effectors, including STAT1 and STAT3.
CC {ECO:0000250|UniProtKB:P01588}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by
CC liver of fetal or neonatal mammals.
CC -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}.
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DR EMBL; Z24681; CAA80848.1; -; mRNA.
DR EMBL; L10610; AAA31518.1; -; mRNA.
DR PIR; I46401; I46401.
DR RefSeq; NP_001019908.1; NM_001024737.1.
DR AlphaFoldDB; P33709; -.
DR SMR; P33709; -.
DR STRING; 9940.ENSOARP00000017231; -.
DR GlyCosmos; P33709; 3 sites, No reported glycans.
DR PaxDb; 9940-ENSOARP00000017231; -.
DR Ensembl; ENSOART00000017473.1; ENSOARP00000017231.1; ENSOARG00000016044.1.
DR Ensembl; ENSOART00020025463; ENSOARP00020021115; ENSOARG00020016587.
DR GeneID; 443302; -.
DR KEGG; oas:443302; -.
DR CTD; 2056; -.
DR eggNOG; ENOG502RXRC; Eukaryota.
DR HOGENOM; CLU_110946_0_0_1; -.
DR OMA; AMEFPRL; -.
DR OrthoDB; 4338688at2759; -.
DR Proteomes; UP000002356; Chromosome 24.
DR Bgee; ENSOARG00000016044; Expressed in embryo and 3 other cell types or tissues.
DR GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR GO; GO:0005615; C:extracellular space; IEA:Ensembl.
DR GO; GO:0005125; F:cytokine activity; IEA:Ensembl.
DR GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; IEA:Ensembl.
DR GO; GO:0071474; P:cellular hyperosmotic response; IEA:Ensembl.
DR GO; GO:0007566; P:embryo implantation; IEA:Ensembl.
DR GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
DR GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW.
DR GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0042541; P:hemoglobin biosynthetic process; IEA:Ensembl.
DR GO; GO:0033028; P:myeloid cell apoptotic process; IEA:Ensembl.
DR GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; IEA:Ensembl.
DR GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; IEA:Ensembl.
DR GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:Ensembl.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IEA:Ensembl.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR019767; EPO/TPO_CS.
DR InterPro; IPR001323; EPO_TPO.
DR InterPro; IPR003013; Erythroptn.
DR PANTHER; PTHR10370; ERYTHROPOIETIN; 1.
DR PANTHER; PTHR10370:SF0; ERYTHROPOIETIN; 1.
DR Pfam; PF00758; EPO_TPO; 1.
DR PIRSF; PIRSF001951; EPO; 1.
DR PRINTS; PR00272; ERYTHROPTN.
DR SUPFAM; SSF47266; 4-helical cytokines; 1.
DR PROSITE; PS00817; EPO_TPO; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000250"
FT CHAIN 28..194
FT /note="Erythropoietin"
FT /id="PRO_0000008409"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 65
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 34..189
FT /evidence="ECO:0000250"
FT DISULFID 56..60
FT /evidence="ECO:0000250"
FT CONFLICT 16
FT /note="F -> L (in Ref. 2; AAA31518)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="L -> P (in Ref. 2; AAA31518)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 194 AA; 21335 MW; C025AAB0528131A9 CRC64;
MGARDCTPLL LLLLSFLLFP LGLPVLGAPP RLICDSRVLE RYILEAREAE NATMGCAEGC
SFSENITVPD TKVNFYAWKR MEVQQQALEV WQGLALLSEA IFRGQALLAN ASQPCEALRL
HVDKAVSGLR SLTSLLRALG AQKEAIPLPD ATPSAAPLRI FTVDALSKLF RIYSNFLRGK
LTLYTGEACR RGDR
//
