ID ETS1B_CHICK Reviewed; 485 AA.
AC P15062;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 24-JAN-2024, entry version 139.
DE RecName: Full=Transforming protein p68/c-ets-1;
GN Name=ETS1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2841475; DOI=10.1128/jvi.62.9.3233-3241.1988;
RA Leprince D., Duterque-Coquillaud M., Li R.-P., Henry C., Flourens A.,
RA Debuire B., Stehelin D.;
RT "Alternative splicing within the chicken c-ets-1 locus: implications for
RT transduction within the E26 retrovirus of the c-ets proto-oncogene.";
RL J. Virol. 62:3233-3241(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3284179; DOI=10.1016/0042-6822(88)90624-1;
RA Watson D.K., McWilliams M.J., Papas T.S.;
RT "Molecular organization of the chicken ets locus.";
RL Virology 164:99-105(1988).
RN [3]
RP ALTERNATIVE PROMOTER USAGE.
RX PubMed=8268721;
RA Crepieux P., Leprince D., Flourens A., Albagli O., Ferreira E.,
RA Stehelin D.;
RT "The two functionally distinct amino termini of chicken c-ets-1 products
RT arise from alternative promoter usage.";
RL Gene Expr. 3:215-225(1993).
CC -!- FUNCTION: Transcription factor. Directly controls the expression of
CC cytokine and chemokine genes in a wide variety of different cellular
CC contexts. {ECO:0000250|UniProtKB:P14921}.
CC -!- ACTIVITY REGULATION: Autoinhibited by a module composed of four alpha
CC helices (HI-1, HI-2, H4, and H5) that flank the DNA-binding ETS domain,
CC reducing the affinity for DNA. {ECO:0000250|UniProtKB:P27577}.
CC -!- SUBUNIT: Binds DNA as a homodimer; homodimerization is required for
CC transcription activation. {ECO:0000250|UniProtKB:P14921}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P14921}. Cytoplasm
CC {ECO:0000250|UniProtKB:P14921}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage; Named isoforms=2;
CC Name=p68;
CC IsoId=P15062-1; Sequence=Displayed;
CC Name=p54;
CC IsoId=P13474-1; Sequence=External;
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA48668.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M21823; AAA48669.1; -; mRNA.
DR EMBL; AH002441; AAA48668.1; ALT_INIT; Genomic_DNA.
DR PIR; A28875; TVCHET.
DR RefSeq; XP_015153455.1; XM_015297969.1. [P15062-1]
DR AlphaFoldDB; P15062; -.
DR SMR; P15062; -.
DR STRING; 9031.ENSGALP00000001715; -.
DR PaxDb; 9031-ENSGALP00000001717; -.
DR GeneID; 396235; -.
DR CTD; 2113; -.
DR VEuPathDB; HostDB:geneid_396235; -.
DR eggNOG; KOG3806; Eukaryota.
DR InParanoid; P15062; -.
DR PhylomeDB; P15062; -.
DR Proteomes; UP000000539; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0010595; P:positive regulation of endothelial cell migration; ISS:UniProtKB.
DR GO; GO:0045765; P:regulation of angiogenesis; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd08542; SAM_PNT-ETS-1; 1.
DR Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR045688; Ets1_N_flank.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR003118; Pointed_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR041886; SAM_PNT-ETS-1.
DR InterPro; IPR016311; Transform_prot_C-ets.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; ETS; 1.
DR PANTHER; PTHR11849:SF209; PROTEIN C-ETS-1; 1.
DR Pfam; PF00178; Ets; 1.
DR Pfam; PF19525; Ets1_N_flank; 1.
DR Pfam; PF02198; SAM_PNT; 1.
DR PIRSF; PIRSF001698; Transforming_factor_C-ets; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SMART; SM00251; SAM_PNT; 1.
DR SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR PROSITE; PS51433; PNT; 1.
PE 2: Evidence at transcript level;
KW Alternative promoter usage; Cytoplasm; DNA-binding; Nucleus;
KW Proto-oncogene; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..485
FT /note="Transforming protein p68/c-ets-1"
FT /id="PRO_0000204073"
FT DOMAIN 95..180
FT /note="PNT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT DNA_BIND 379..459
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 174..287
FT /note="Activation domain; required for transcription
FT activation"
FT /evidence="ECO:0000250|UniProtKB:P14921"
FT REGION 348..356
FT /note="Helix HI-1"
FT /evidence="ECO:0000250|UniProtKB:P27577"
FT REGION 367..374
FT /note="Helix HI-2"
FT /evidence="ECO:0000250|UniProtKB:P27577"
FT REGION 462..466
FT /note="Helix H4"
FT /evidence="ECO:0000250|UniProtKB:P27577"
FT REGION 470..476
FT /note="Helix H5"
FT /evidence="ECO:0000250|UniProtKB:P27577"
FT CONFLICT 220
FT /note="S -> C (in Ref. 2; AAA48668)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="P -> R (in Ref. 2; AAA48668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 485 AA; 55021 MW; 81576B43BEF62648 CRC64;
MMSYYMDTTI GSTGPYPLAR PGVMQGASSC CEDPWMPCRL QSACCPPRSC CPPWDEAAIQ
EVPTGLEHYS TDMECADVPL LTPSSKEMMS QALKATFSGF AKEQQRLGIP KDPQQWTETH
VRDWVMWAVN EFSLKGVDFQ KFCMNGAALC ALGKECFLEL APDFVGDILW EHLEILQKEE
AKPYPANGVN AAYPESRYTS DYFISYGIEH AQCVPPSEFS EPSFITESYQ TLHPISSEEL
LSLKYENDYP SVILRDPVQT DSLQTDYFTI KQEVVTPDNM CMGRASRGKL GGQDSFESIE
SYDSCDRLTQ SWSSQSSFQS LQRVPSYDSF DSEDYPAALP NHKPKGTFKD YVRDRADMNK
DKPVIPAAAL AGYTGSGPIQ LWQFLLELLT DKSCQSFISW TGDGWEFKLS DPDEVARRWG
KRKNKPKMNY EKLSRGLRYY YDKNIIHKTA GKRYVYRFVC DLQSLLGYTP EELHAMLDVK
PDADE
//
