ID ETS3_DROME Reviewed; 490 AA.
AC P29774; Q86P68; Q95T62; Q95TB2; Q9VRU3; Q9VRU4; Q9VRU5;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2003, sequence version 3.
DT 27-MAR-2024, entry version 173.
DE RecName: Full=DNA-binding protein D-ETS-3;
GN Name=Ets65A; Synonyms=ETS3; ORFNames=CG7018;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP SEQUENCE REVISION.
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 314-375, FUNCTION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S; TISSUE=Larva;
RX PubMed=1577186; DOI=10.1016/0012-1606(92)90225-6;
RA Chen T., Bunting M., Karim F.D., Thummel C.S.;
RT "Isolation and characterization of five Drosophila genes that encode an
RT ets-related DNA binding domain.";
RL Dev. Biol. 151:176-191(1992).
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=P29774-1; Sequence=Displayed;
CC Name=B;
CC IsoId=P29774-2; Sequence=VSP_001470, VSP_001471;
CC -!- TISSUE SPECIFICITY: Embryonic ventral nervous system, higher in the
CC thoracic than abdominal segments. {ECO:0000269|PubMed:1577186}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:1577186}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50697.2; -; Genomic_DNA.
DR EMBL; AE014296; AAF50696.2; -; Genomic_DNA.
DR EMBL; AY060316; AAL25355.1; -; mRNA.
DR EMBL; AY060250; AAR88562.1; -; mRNA.
DR EMBL; BT003453; AAO39456.1; -; mRNA.
DR EMBL; M88473; AAA28450.2; -; Genomic_DNA.
DR PIR; S28820; S28820.
DR RefSeq; NP_523945.2; NM_079221.3. [P29774-2]
DR RefSeq; NP_729142.1; NM_168156.2. [P29774-1]
DR AlphaFoldDB; P29774; -.
DR SMR; P29774; -.
DR BioGRID; 64155; 71.
DR IntAct; P29774; 71.
DR STRING; 7227.FBpp0312433; -.
DR PaxDb; 7227-FBpp0076762; -.
DR DNASU; 38700; -.
DR EnsemblMetazoa; FBtr0077054; FBpp0076762; FBgn0005658. [P29774-1]
DR EnsemblMetazoa; FBtr0077055; FBpp0076763; FBgn0005658. [P29774-2]
DR GeneID; 38700; -.
DR KEGG; dme:Dmel_CG7018; -.
DR AGR; FB:FBgn0005658; -.
DR CTD; 38700; -.
DR FlyBase; FBgn0005658; Ets65A.
DR VEuPathDB; VectorBase:FBgn0005658; -.
DR eggNOG; KOG3806; Eukaryota.
DR HOGENOM; CLU_022965_0_0_1; -.
DR InParanoid; P29774; -.
DR OMA; WTNPSAN; -.
DR OrthoDB; 2882052at2759; -.
DR Reactome; R-DME-2559585; Oncogene Induced Senescence.
DR BioGRID-ORCS; 38700; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 38700; -.
DR PRO; PR:P29774; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0005658; Expressed in presumptive embryonic/larval central nervous system and 28 other cell types or tissues.
DR ExpressionAtlas; P29774; baseline and differential.
DR Genevisible; P29774; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; NAS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849:SF304; DNA-BINDING PROTEIN D-ETS-3-RELATED; 1.
DR PANTHER; PTHR11849; ETS; 1.
DR Pfam; PF00178; Ets; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..490
FT /note="DNA-binding protein D-ETS-3"
FT /id="PRO_0000204107"
FT DNA_BIND 317..397
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 190..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 207..255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 271..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..233
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_001470"
FT VAR_SEQ 234..260
FT /note="ISGSKSSNTSGTGGGASASGGGGSALY -> MLDIKSSADYLSRSTGSFSNF
FT SMLFAD (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_001471"
SQ SEQUENCE 490 AA; 52055 MW; B5BB281B13DDEA97 CRC64;
MYENSCSYQT ALDLKRVSPP TLAQVKTEEC LALGQCSPEW TSYRFHQSTF EQLKQSVEKA
KAALQDRSSF FGASSAFSDI YSSQRLTDSL DTLPVQSGNG GGNCLGLPHN PNVGVGVAGV
LNYNAVSSST AGVLSSSGNG VQRHRLDTLQ PPSGCSPAVT QHGVITSSAG QVTSGTLDAV
SAAPALPSLT ASSSSHVEHK VRADKSTLDC ATTSSHAAAP SSSSSASDHQ QGRISGSKSS
NTSGTGGGAS ASGGGGSALY SSYKSSWGSH SSTQSQGYSS NALGIKHDPH SQLRQPDPYQ
MFGPTSSRLA SSGSGQIQLW QFLLELLSDS NNASCITWEG TNGEFKLTDP DEVARRWGER
KSKPNMNYDK LSRALRYYYD KNIMTKVHGK RYAYKFDFQG LAAATQPAAS DPTYKYQSDL
FMTPYHHSAK LSSFMSPHHG MTSSSASIFP SAASWGNWGS PATNLYQPHS MSHVTPSHVA
PHLSSYPHYA
//
