ID EUTS_ECOLI Reviewed; 111 AA.
AC P63746; P76557; Q2MAI6;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 24-JAN-2024, entry version 110.
DE RecName: Full=Bacterial microcompartment shell protein EutS;
DE AltName: Full=Ethanolamine utilization protein EutS;
GN Name=eutS; Synonyms=ypfE; OrderedLocusNames=b2462, JW2446;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3] {ECO:0007744|PDB:3I96, ECO:0007744|PDB:3IA0}
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS), FUNCTION, SUBUNIT, SUBCELLULAR
RP LOCATION, DOMAIN, AND MUTAGENESIS OF GLY-39.
RC STRAIN=K12;
RX PubMed=20044574; DOI=10.1126/science.1179513;
RA Tanaka S., Sawaya M.R., Yeates T.O.;
RT "Structure and mechanisms of a protein-based organelle in Escherichia
RT coli.";
RL Science 327:81-84(2010).
CC -!- FUNCTION: A component of the bacterial microcompartment (BMC) shell
CC dedicated to ethanolamine degradation. Its unusual hexameric shape may
CC help form the BMC shell (Probable). Targets at least 2 proteins (EutC
CC and EutE) to the interior of the BMC (By similarity). Proteins such as
CC EutS containing circularly permuted BMC domains may play a key role in
CC conferring heterogeneity and flexibility in this BMC (Probable).
CC {ECO:0000250|UniProtKB:Q9ZFV7, ECO:0000305,
CC ECO:0000305|PubMed:20044574}.
CC -!- PATHWAY: Amine and polyamine degradation; ethanolamine degradation.
CC -!- SUBUNIT: Homohexamer with a central pore; the hexamer is not symmetric,
CC it is bent by about 40 degrees; bending depends on Gly-39
CC (PubMed:20044574). Interacts with the N-terminus of EutC and of EutE,
CC targeting them to the interior of the BMC (By similarity).
CC {ECO:0000250|UniProtKB:Q9ZFV7, ECO:0000269|PubMed:20044574}.
CC -!- INTERACTION:
CC P63746; P63746: eutS; NbExp=2; IntAct=EBI-1128985, EBI-1128985;
CC -!- SUBCELLULAR LOCATION: Bacterial microcompartment
CC {ECO:0000305|PubMed:20044574}.
CC -!- DOMAIN: One side of the hexamer is concave and lined by hydrophobic
CC residues, the other side has a slightly protruding, 6-stranded beta-
CC barrel. {ECO:0000269|PubMed:20044574}.
CC -!- SIMILARITY: Belongs to the EutS/PduU family. {ECO:0000255|PROSITE-
CC ProRule:PRU01279}.
CC -!- CAUTION: In strain MG1655 the eut operon is interrupted by the CPZ-55
CC prophage, encoding 9 genes situated between eutA and eutB, which are
CC translated in the other direction. CPZ-55 may prevent expression of the
CC eut operon in strain MG1655. Strain W3110 does not have this prophage
CC element and should be able to express the operon.
CC {ECO:0000305|PubMed:9278503}.
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DR EMBL; U00096; AAC75515.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76720.1; -; Genomic_DNA.
DR RefSeq; NP_416957.4; NC_000913.3.
DR RefSeq; WP_000356956.1; NZ_STEB01000051.1.
DR PDB; 3I96; X-ray; 1.65 A; A/B/C=1-111.
DR PDB; 3IA0; X-ray; 2.50 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v=1-111.
DR PDBsum; 3I96; -.
DR PDBsum; 3IA0; -.
DR AlphaFoldDB; P63746; -.
DR SMR; P63746; -.
DR BioGRID; 4260922; 8.
DR BioGRID; 851275; 1.
DR IntAct; P63746; 9.
DR STRING; 511145.b2462; -.
DR PaxDb; 511145-b2462; -.
DR EnsemblBacteria; AAC75515; AAC75515; b2462.
DR GeneID; 75204266; -.
DR GeneID; 946936; -.
DR KEGG; ecj:JW2446; -.
DR KEGG; eco:b2462; -.
DR PATRIC; fig|1411691.4.peg.4278; -.
DR EchoBASE; EB3944; -.
DR eggNOG; COG4810; Bacteria.
DR HOGENOM; CLU_143326_0_0_6; -.
DR InParanoid; P63746; -.
DR OMA; AMIAIND; -.
DR OrthoDB; 5457140at2; -.
DR PhylomeDB; P63746; -.
DR BioCyc; EcoCyc:G7292-MONOMER; -.
DR UniPathway; UPA00560; -.
DR EvolutionaryTrace; P63746; -.
DR PRO; PR:P63746; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031471; C:ethanolamine degradation polyhedral organelle; ISS:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0005198; F:structural molecule activity; ISS:EcoCyc.
DR GO; GO:0046336; P:ethanolamine catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0034214; P:protein hexamerization; IDA:EcoCyc.
DR CDD; cd07046; BMC_PduU-EutS; 1.
DR Gene3D; 3.30.70.1710; -; 1.
DR InterPro; IPR044870; BMC_CP.
DR InterPro; IPR000249; BMC_dom.
DR InterPro; IPR037233; CcmK-like_sf.
DR InterPro; IPR009307; EutS/PduU/CutR.
DR PANTHER; PTHR40449:SF2; BACTERIAL MICROCOMPARTMENT SHELL PROTEIN EUTS; 1.
DR PANTHER; PTHR40449; ETHANOLAMINE UTILIZATION PROTEIN EUTS; 1.
DR Pfam; PF00936; BMC; 1.
DR PIRSF; PIRSF012296; EutS_PduU; 1.
DR SMART; SM00877; BMC; 1.
DR SUPFAM; SSF143414; CcmK-like; 1.
DR PROSITE; PS51931; BMC_CP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial microcompartment; Reference proteome.
FT CHAIN 1..111
FT /note="Bacterial microcompartment shell protein EutS"
FT /id="PRO_0000201520"
FT DOMAIN 5..103
FT /note="BMC circularly permuted"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01279"
FT MUTAGEN 39
FT /note="G->V: Crystallizes as a symmetric hexamer, migrates
FT more slowly in native gels."
FT /evidence="ECO:0000269|PubMed:20044574"
FT STRAND 8..12
FT /evidence="ECO:0007829|PDB:3I96"
FT STRAND 15..23
FT /evidence="ECO:0007829|PDB:3I96"
FT HELIX 27..33
FT /evidence="ECO:0007829|PDB:3I96"
FT STRAND 39..48
FT /evidence="ECO:0007829|PDB:3I96"
FT HELIX 51..62
FT /evidence="ECO:0007829|PDB:3I96"
FT STRAND 63..70
FT /evidence="ECO:0007829|PDB:3I96"
FT TURN 72..74
FT /evidence="ECO:0007829|PDB:3I96"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:3I96"
FT HELIX 83..101
FT /evidence="ECO:0007829|PDB:3I96"
FT STRAND 109..111
FT /evidence="ECO:0007829|PDB:3I96"
SQ SEQUENCE 111 AA; 11650 MW; FBA3F8401EAE26C3 CRC64;
MDKERIIQEF VPGKQVTLAH LIAHPGEELA KKIGVPDAGA IGIMTLTPGE TAMIAGDLAL
KAADVHIGFL DRFSGALVIY GSVGAVEEAL SQTVSGLGRL LNYTLCEMTK S
//
