ID EXOS8_BOVIN Reviewed; 276 AA.
AC Q2KHU3;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=Exosome complex component RRP43;
DE AltName: Full=Exosome component 8;
DE AltName: Full=Ribosomal RNA-processing protein 43;
GN Name=EXOSC8;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Heart ventricle;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Non-catalytic component of the RNA exosome complex which has
CC 3'->5' exoribonuclease activity and participates in a multitude of
CC cellular RNA processing and degradation events. In the nucleus, the RNA
CC exosome complex is involved in proper maturation of stable RNA species
CC such as rRNA, snRNA and snoRNA, in the elimination of RNA processing
CC by-products and non-coding 'pervasive' transcripts, such as antisense
CC RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs
CC with processing defects, thereby limiting or excluding their export to
CC the cytoplasm. The RNA exosome may be involved in Ig class switch
CC recombination (CSR) and/or Ig variable region somatic hypermutation
CC (SHM) by targeting AICDA deamination activity to transcribed dsDNA
CC substrates. In the cytoplasm, the RNA exosome complex is involved in
CC general mRNA turnover and specifically degrades inherently unstable
CC mRNAs containing AU-rich elements (AREs) within their 3' untranslated
CC regions, and in RNA surveillance pathways, preventing translation of
CC aberrant mRNAs. It seems to be involved in degradation of histone mRNA.
CC The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9)
CC is proposed to play a pivotal role in the binding and presentation of
CC RNA for ribonucleolysis, and to serve as a scaffold for the association
CC with catalytic subunits and accessory proteins or complexes. EXOSC8
CC binds to ARE-containing RNAs (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA exosome complex. Specifically part of the
CC catalytically inactive RNA exosome core (Exo-9) complex which is
CC believed to associate with catalytic subunits EXOSC10, and DIS3 or
CC DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms.
CC Exo-9 is formed by a hexameric ring of RNase PH domain-containing
CC subunits specifically containing the heterodimers EXOSC4-EXOSC9,
CC EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and peripheral S1 domain-containing
CC components EXOSC1, EXOSC2 and EXOSC3 located on the top of the ring
CC structure (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus, nucleolus
CC {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RNase PH family. {ECO:0000305}.
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DR EMBL; BC112881; AAI12882.1; -; mRNA.
DR RefSeq; NP_001073074.1; NM_001079606.2.
DR AlphaFoldDB; Q2KHU3; -.
DR SMR; Q2KHU3; -.
DR STRING; 9913.ENSBTAP00000015733; -.
DR PaxDb; 9913-ENSBTAP00000015733; -.
DR Ensembl; ENSBTAT00000015733.6; ENSBTAP00000015733.5; ENSBTAG00000011855.6.
DR GeneID; 532080; -.
DR KEGG; bta:532080; -.
DR CTD; 11340; -.
DR VEuPathDB; HostDB:ENSBTAG00000011855; -.
DR VGNC; VGNC:28662; EXOSC8.
DR eggNOG; KOG1613; Eukaryota.
DR GeneTree; ENSGT00950000183130; -.
DR HOGENOM; CLU_038194_3_1_1; -.
DR InParanoid; Q2KHU3; -.
DR OMA; RIWYQPP; -.
DR OrthoDB; 21598at2759; -.
DR TreeFam; TF320415; -.
DR Reactome; R-BTA-429958; mRNA decay by 3' to 5' exoribonuclease.
DR Reactome; R-BTA-450385; Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
DR Reactome; R-BTA-450513; Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA.
DR Reactome; R-BTA-450604; KSRP (KHSRP) binds and destabilizes mRNA.
DR Reactome; R-BTA-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Proteomes; UP000009136; Chromosome 12.
DR Bgee; ENSBTAG00000011855; Expressed in oocyte and 106 other cell types or tissues.
DR ExpressionAtlas; Q2KHU3; baseline and differential.
DR GO; GO:0000177; C:cytoplasmic exosome (RNase complex); IBA:GO_Central.
DR GO; GO:0000178; C:exosome (RNase complex); ISS:UniProtKB.
DR GO; GO:0000176; C:nuclear exosome (RNase complex); IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0043928; P:exonucleolytic catabolism of deadenylated mRNA; IBA:GO_Central.
DR GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IBA:GO_Central.
DR GO; GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IBA:GO_Central.
DR GO; GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IBA:GO_Central.
DR GO; GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IBA:GO_Central.
DR GO; GO:0034427; P:nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'; IBA:GO_Central.
DR GO; GO:0034473; P:U1 snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0034475; P:U4 snRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0034476; P:U5 snRNA 3'-end processing; IBA:GO_Central.
DR CDD; cd11369; RNase_PH_RRP43; 1.
DR Gene3D; 3.30.230.70; GHMP Kinase, N-terminal domain; 1.
DR InterPro; IPR001247; ExoRNase_PH_dom1.
DR InterPro; IPR015847; ExoRNase_PH_dom2.
DR InterPro; IPR036345; ExoRNase_PH_dom2_sf.
DR InterPro; IPR027408; PNPase/RNase_PH_dom_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR033196; Rrp43.
DR PANTHER; PTHR11097:SF9; EXOSOME COMPLEX COMPONENT RRP43; 1.
DR PANTHER; PTHR11097; EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN; 1.
DR Pfam; PF01138; RNase_PH; 1.
DR Pfam; PF03725; RNase_PH_C; 1.
DR SUPFAM; SSF55666; Ribonuclease PH domain 2-like; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Exosome; Nucleus; Reference proteome; RNA-binding;
KW rRNA processing.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q96B26"
FT CHAIN 2..276
FT /note="Exosome complex component RRP43"
FT /id="PRO_0000278841"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q96B26"
SQ SEQUENCE 276 AA; 29990 MW; EC64BC9BF2128B0D CRC64;
MAAGFKTVEP LEYYRRFLKE NCRPDGRELG EFRTTTVNVG SIGTADGSAL VKLGNTTVIC
GIKAEFGAPP TDAPDKGYVV PNVDLSPLCS SRFRSGPPGE EAQVASQFIA DVIENSQIIQ
KEDLCISSGK LAWVLYCDLI CLNHDGNILD ACTFALLAAL KNVQLPEVTI NEETALAEVN
LKKKSCLNIR THPVATSFAV FDDTLLIVDP TEEEEHLATG TLTVVMDEEG RLCCLHKPGG
SGLTGAKLQD CMSRAVTRHK EVKKLMDEVF KSMKPK
//
