ID EXPB3_ARATH Reviewed; 264 AA.
AC Q9M0I2;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 05-MAR-2002, sequence version 2.
DT 24-JAN-2024, entry version 159.
DE RecName: Full=Expansin-B3;
DE Short=At-EXPB3;
DE Short=AtEXPB3;
DE AltName: Full=Ath-ExpBeta-1.6;
DE AltName: Full=Beta-expansin-3;
DE Flags: Precursor;
GN Name=EXPB3; OrderedLocusNames=At4g28250; ORFNames=F26K10.130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NOMENCLATURE.
RX PubMed=15604683; DOI=10.1007/s11103-004-0158-6;
RA Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J.,
RA Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C.,
RA Voesenek L.A.C.;
RT "Nomenclature for members of the expansin superfamily of genes and
RT proteins.";
RL Plant Mol. Biol. 55:311-314(2004).
CC -!- FUNCTION: May cause loosening and extension of plant cell walls by
CC disrupting non-covalent bonding between cellulose microfibrils and
CC matrix glucans. No enzymatic activity has been found (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Peripheral
CC membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9M0I2-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the expansin family. Expansin B subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB79627.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=EXPANSIN homepage;
CC URL="https://www.dept.psu.edu/biology/groups/expansins/index.htm";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL161572; CAB79627.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85458.1; -; Genomic_DNA.
DR EMBL; AY039986; AAK64163.1; -; mRNA.
DR EMBL; AY133801; AAM91735.1; -; mRNA.
DR RefSeq; NP_567803.1; NM_118965.4. [Q9M0I2-1]
DR AlphaFoldDB; Q9M0I2; -.
DR SMR; Q9M0I2; -.
DR BioGRID; 14227; 6.
DR IntAct; Q9M0I2; 6.
DR STRING; 3702.Q9M0I2; -.
DR PaxDb; 3702-AT4G28250-1; -.
DR ProteomicsDB; 221820; -. [Q9M0I2-1]
DR EnsemblPlants; AT4G28250.1; AT4G28250.1; AT4G28250. [Q9M0I2-1]
DR GeneID; 828940; -.
DR Gramene; AT4G28250.1; AT4G28250.1; AT4G28250. [Q9M0I2-1]
DR KEGG; ath:AT4G28250; -.
DR Araport; AT4G28250; -.
DR TAIR; AT4G28250; EXPB3.
DR eggNOG; ENOG502QPVQ; Eukaryota.
DR HOGENOM; CLU_027462_1_2_1; -.
DR InParanoid; Q9M0I2; -.
DR OMA; WGASWCI; -.
DR OrthoDB; 889108at2759; -.
DR PhylomeDB; Q9M0I2; -.
DR PRO; PR:Q9M0I2; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9M0I2; baseline and differential.
DR Genevisible; Q9M0I2; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0019953; P:sexual reproduction; IEA:InterPro.
DR GO; GO:0006949; P:syncytium formation; IEP:TAIR.
DR CDD; cd22275; DPBB_EXPB_N; 1.
DR Gene3D; 2.60.40.760; Expansin, cellulose-binding-like domain; 1.
DR Gene3D; 2.40.40.10; RlpA-like domain; 1.
DR InterPro; IPR007118; Expan_Lol_pI.
DR InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR InterPro; IPR007117; Expansin_CBD.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR005795; LolPI.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR PANTHER; PTHR31692; EXPANSIN-B3; 1.
DR PANTHER; PTHR31692:SF5; EXPANSIN-B3; 1.
DR Pfam; PF03330; DPBB_1; 1.
DR Pfam; PF01357; Expansin_C; 1.
DR PRINTS; PR01225; EXPANSNFAMLY.
DR PRINTS; PR00829; LOLP1ALLERGN.
DR SMART; SM00837; DPBB_1; 1.
DR SUPFAM; SSF50685; Barwin-like endoglucanases; 1.
DR SUPFAM; SSF49590; PHL pollen allergen; 1.
DR PROSITE; PS50843; EXPANSIN_CBD; 1.
DR PROSITE; PS50842; EXPANSIN_EG45; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall; Cell wall biogenesis/degradation;
KW Disulfide bond; Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..264
FT /note="Expansin-B3"
FT /id="PRO_0000008709"
FT DOMAIN 54..162
FT /note="Expansin-like EG45"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DOMAIN 175..256
FT /note="Expansin-like CBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT DISULFID 57..86
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 89..157
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 94..100
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
SQ SEQUENCE 264 AA; 28425 MW; 627A6AEA5127C716 CRC64;
MQLFPVMLAT LCIVLQLLIG SSALATTNRH VSNSHWLPAV ATWYGSPNGD GSDGGACGYG
TLVDVKPLHA RVGAVNPILF KNGEGCGACY KVRCLDKSIC SRRAVTVIIT DECPGCSKTS
THFDLSGAVF GRLAIAGESG PLRNRGLIPV IYRRTACKYR GKNIAFHVNE GSTDFWLSLL
VEFEDGEGDI GSMHIRQAGA REWLEMKHVW GANWCIIGGP LKGPFSIKLT TLSAGKTLSA
TDVVPRNWAP KATYSSRLNF SPVL
//
