ID F0GVK7_9FIRM Unreviewed; 603 AA.
AC F0GVK7;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 24-JAN-2024, entry version 58.
DE RecName: Full=1,4-alpha-glucan branching enzyme {ECO:0000256|ARBA:ARBA00012541};
DE EC=2.4.1.18 {ECO:0000256|ARBA:ARBA00012541};
GN Name=glgB {ECO:0000313|EMBL:EGC82124.1};
GN ORFNames=HMPREF9290_1062 {ECO:0000313|EMBL:EGC82124.1};
OS Anaerococcus prevotii ACS-065-V-Col13.
OC Bacteria; Bacillota; Tissierellia; Tissierellales; Peptoniphilaceae;
OC Anaerococcus.
OX NCBI_TaxID=879305 {ECO:0000313|EMBL:EGC82124.1, ECO:0000313|Proteomes:UP000005286};
RN [1] {ECO:0000313|EMBL:EGC82124.1, ECO:0000313|Proteomes:UP000005286}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ACS-065-V-Col13 {ECO:0000313|EMBL:EGC82124.1,
RC ECO:0000313|Proteomes:UP000005286};
RA Durkin A.S., Madupu R., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position.
CC {ECO:0000256|ARBA:ARBA00002953}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000256|ARBA:ARBA00000826};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004964}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000256|ARBA:ARBA00009000}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGC82124.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AEXM01000017; EGC82124.1; -; Genomic_DNA.
DR RefSeq; WP_004834845.1; NZ_AEXM01000017.1.
DR AlphaFoldDB; F0GVK7; -.
DR STRING; 879305.HMPREF9290_1062; -.
DR PATRIC; fig|879305.3.peg.837; -.
DR eggNOG; COG0296; Bacteria.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000005286; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd11322; AmyAc_Glg_BE; 1.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR01515; branching_enzym; 1.
DR PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR PANTHER; PTHR43651:SF3; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycogen metabolism {ECO:0000256|ARBA:ARBA00022600};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000313|EMBL:EGC82124.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000005286};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EGC82124.1}.
FT DOMAIN 144..484
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 298
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
FT ACT_SITE 341
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
SQ SEQUENCE 603 AA; 70595 MW; 75E5066A5F9F1C37 CRC64;
MKVNIIETNH SYIKDYINGH SSAGADIWGA QKDCDGYYIF RVLAPNADEV YIKGDFTSWQ
NVAMTKNYEY GYFFIRLKAN VGDYYKYVVN HKGNFVEKTD PFARAMDKEG DFASIITDDS
YEFADDTFIK NRGKNFDKPL NIYEIHIGSW KRYGDNVNFL DIVDDLVSYV KKMNYTHVEI
MPITEYPYYP SWGYQSTGFF ATSSRFGSPV DLKKFVDILH QNGIGVILDM VAVHFASDFY
GLDHFDGTGM YESVYPDLKY SEWGSNNFDY SKGHVRSFMK SAFSYWIENF HFDGIRIDAV
SYMIHYNGNK NRGVHQDNIS FIKDLNKTIA SCHPDVMLIA EDSSDYPKVT ASVDEDGLGF
DYKWDLGWMN DTLRYFKTDS MNRVDHQQNI NFSMFYFYNE KFVLPLSHDE VVHLKGAVVN
KMNGSYEEKF KQLKLLYTYQ ITHPGKKLNF MGNDIATFDE WNESGSINWD ILKFPVHDSF
NRFIKDLNYL YKTEKAFFEK DFDEEGFSWK VVDDNVNSVF AYERKSGDDR FLVVLNMTNT
YKASYKIPYD EELEFVEVID SLSEIYGNSR WDKRNIKIEK GENLALELWE YEALVLKIKT
YEL
//
