ID F0V879_NEOCL Unreviewed; 512 AA.
AC F0V879;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE SubName: Full=Radical SAM enzyme, Cfr family, related {ECO:0000313|EMBL:CBZ49920.1};
GN ORFNames=NCLIV_004040 {ECO:0000313|EMBL:CBZ49920.1};
OS Neospora caninum (strain Liverpool).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Neospora.
OX NCBI_TaxID=572307 {ECO:0000313|EMBL:CBZ49920.1, ECO:0000313|Proteomes:UP000007494};
RN [1] {ECO:0000313|Proteomes:UP000007494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Liverpool {ECO:0000313|Proteomes:UP000007494};
RX PubMed=22457617; DOI=10.1371/journal.ppat.1002567;
RA Reid A.J., Vermont S.J., Cotton J.A., Harris D., Hill-Cawthorne G.A.,
RA Konen-Waisman S., Latham S.M., Mourier T., Norton R., Quail M.A.,
RA Sanders M., Shanmugam D., Sohal A., Wasmuth J.D., Brunk B., Grigg M.E.,
RA Howard J.C., Parkinson J., Roos D.S., Trees A.J., Berriman M., Pain A.,
RA Wastling J.M.;
RT "Comparative genomics of the apicomplexan parasites Toxoplasma gondii and
RT Neospora caninum: Coccidia differing in host range and transmission
RT strategy.";
RL PLoS Pathog. 8:e1002567-e1002567(2012).
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DR EMBL; FR823381; CBZ49920.1; -; Genomic_DNA.
DR RefSeq; XP_003879955.1; XM_003879906.1.
DR AlphaFoldDB; F0V879; -.
DR GeneID; 13445969; -.
DR VEuPathDB; ToxoDB:NCLIV_004040; -.
DR eggNOG; ENOG502QQ98; Eukaryota.
DR InParanoid; F0V879; -.
DR OrthoDB; 276083at2759; -.
DR Proteomes; UP000007494; Chromosome Ib.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR040072; Methyltransferase_A.
DR InterPro; IPR007197; rSAM.
DR PANTHER; PTHR30544; 23S RRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR30544:SF8; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000007494};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 486..507
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 86..328
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 16..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..66
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 344..369
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 372..386
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..432
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 512 AA; 56748 MW; 5FD6F24CB206A2E4 CRC64;
MRYGAVAYAL FPEAKKKPRD ACVETSEAQP REEKGTAAEE EDEKEEDEEK EEDEEKEEDE
EKEEDEERGE SVKLGKTSDV ARPFKSNRRA TVCLSAQVGC QMGCTFCATG TMGKKRNLAE
WEILEQLYHA SRVEKIRNIV FMGMGEPLDN YNNVVASVRF MTQPNKFAIG GHHVCISTVG
LPHRIRQLAS DLPTCRLALS LHAPDQPTRL KLMPRAAAGW KLDRVLDATD EFVKQQKRVN
STAMKNIGLL VEYIMIQDVN DTIDQAHALG RILQPRADAV IVNLIPYNPT EVPYDYKPST
PERVDEFLRI LRQDYSIKVL VRQTLGQDID SACGQLVVRA GKDKSGEKVP ERVARASHGA
ARTRDARDAT PVESSAGSQW PFSSRDRESA CASKAPSGAA QISTRQKCSR SRSDERETDS
DDASDDGRDK HSAVPLSSWW QDVKALPALM QSCKGMCADS LWTLATDAER EDEEEAVAAT
HGKRTLLAAS VALLAGVVAA GVANWFLRRA RV
//
