ID F0Y1E3_AURAN Unreviewed; 538 AA.
AC F0Y1E3;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093};
DE EC=3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093};
GN Name=NAG1 {ECO:0000313|EMBL:EGB10810.1};
GN ORFNames=AURANDRAFT_22610 {ECO:0000313|EMBL:EGB10810.1};
OS Aureococcus anophagefferens (Harmful bloom alga).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Pelagophyceae; Pelagomonadales;
OC Aureococcus.
OX NCBI_TaxID=44056 {ECO:0000313|Proteomes:UP000002729};
RN [1] {ECO:0000313|EMBL:EGB10810.1, ECO:0000313|Proteomes:UP000002729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP 1984 {ECO:0000313|Proteomes:UP000002729};
RX PubMed=21368207; DOI=10.1073/pnas.1016106108;
RA Gobler C.J., Berry D.L., Dyhrman S.T., Wilhelm S.W., Salamov A.,
RA Lobanov A.V., Zhang Y., Collier J.L., Wurch L.L., Kustka A.B., Dill B.D.,
RA Shah M., VerBerkmoes N.C., Kuo A., Terry A., Pangilinan J., Lindquist E.A.,
RA Lucas S., Paulsen I.T., Hattenrath-Lehmann T.K., Talmage S.C., Walker E.A.,
RA Koch F., Burson A.M., Marcoval M.A., Tang Y.Z., Lecleir G.R., Coyne K.J.,
RA Berg G.M., Bertrand E.M., Saito M.A., Gladyshev V.N., Grigoriev I.V.;
RT "Niche of harmful alga Aureococcus anophagefferens revealed through
RT ecogenomics.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:4352-4357(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC Evidence={ECO:0000256|ARBA:ARBA00001231,
CC ECO:0000256|PIRNR:PIRNR001093};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family.
CC {ECO:0000256|ARBA:ARBA00006285, ECO:0000256|PIRNR:PIRNR001093}.
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DR EMBL; GL833123; EGB10810.1; -; Genomic_DNA.
DR RefSeq; XP_009034397.1; XM_009036149.1.
DR AlphaFoldDB; F0Y1E3; -.
DR EnsemblProtists; EGB10810; EGB10810; AURANDRAFT_22610.
DR GeneID; 20219633; -.
DR KEGG; aaf:AURANDRAFT_22610; -.
DR eggNOG; KOG2499; Eukaryota.
DR InParanoid; F0Y1E3; -.
DR OMA; NYCVEPP; -.
DR OrthoDB; 178991at2759; -.
DR Proteomes; UP000002729; Unassembled WGS sequence.
DR GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.30.379.10; Chitobiase/beta-hexosaminidase domain 2-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR InterPro; IPR015883; Glyco_hydro_20_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR029018; Hex-like_dom2.
DR InterPro; IPR029019; HEX_eukaryotic_N.
DR PANTHER; PTHR22600; BETA-HEXOSAMINIDASE; 1.
DR PANTHER; PTHR22600:SF21; BETA-HEXOSAMINIDASE A; 1.
DR Pfam; PF00728; Glyco_hydro_20; 1.
DR Pfam; PF14845; Glycohydro_20b2; 1.
DR PIRSF; PIRSF001093; B-hxosamndse_ab_euk; 1.
DR PRINTS; PR00738; GLHYDRLASE20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF55545; beta-N-acetylhexosaminidase-like domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR001093};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR001093};
KW Reference proteome {ECO:0000313|Proteomes:UP000002729};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..15
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 16..538
FT /note="Beta-hexosaminidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012949022"
FT DOMAIN 17..136
FT /note="Beta-hexosaminidase eukaryotic type N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14845"
FT DOMAIN 161..489
FT /note="Glycoside hydrolase family 20 catalytic"
FT /evidence="ECO:0000259|Pfam:PF00728"
FT ACT_SITE 308
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-1"
SQ SEQUENCE 538 AA; 57883 MW; 63A5740F1AA823A3 CRC64;
MHALLLFALC GTARANVWPM PAKFANGSSV ATVDPAKMQL FADGNDTAMV KAELAVAFER
FQRNAFPHAG AKSSAGAVGA VEVTVKDGAA DLQLGVSEAY ELDVPATFYS SGSAVATIQA
DTVFGAYRGL ETLSQLIRFD FGSSSYVVDG APIKISDAPR FPHREILLDS ARHYEPVRVI
EAILDSLAYA KLNTLHWHIS DSQSFPFVAP SHPELAEAAA FSPGERYTAG DVAAVVAYAR
SLGIRVVVEV DTPGHAASFC KSNPDVCPAP DCPEPLLLSN KTFELIGDIF ADFAAVTTDE
IFHLGGDEVR YDCWNKSDAM KAWMAAEKLA TFDDAYAYAV QRVAAGVKAA HGRAAIVWGE
AWDTFGPSMP KSTIFDFWLG GGVSARGVAN ATSHGYRVLW NVGRGSNVGS WRVARRVRKL
RRYLDSLITT WDTMYARDPC TGLTTQQCAL ARRATAPRRR RWGETADPSD IMQTLWPRLA
AIAEVLWSPP HGANATAAAL PRLEAFRCVL EERGVAAAPV SNPLARAAPE GPGSCRSQ
//
