ID F0Y1J6_AURAN Unreviewed; 506 AA.
AC F0Y1J6;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 24-JAN-2024, entry version 45.
DE RecName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex {ECO:0000256|RuleBase:RU003423};
DE EC=2.3.1.- {ECO:0000256|RuleBase:RU003423};
GN Name=DLA1 {ECO:0000313|EMBL:EGB10928.1};
GN ORFNames=AURANDRAFT_58776 {ECO:0000313|EMBL:EGB10928.1};
OS Aureococcus anophagefferens (Harmful bloom alga).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Pelagophyceae; Pelagomonadales;
OC Aureococcus.
OX NCBI_TaxID=44056 {ECO:0000313|Proteomes:UP000002729};
RN [1] {ECO:0000313|EMBL:EGB10928.1, ECO:0000313|Proteomes:UP000002729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP 1984 {ECO:0000313|Proteomes:UP000002729};
RX PubMed=21368207; DOI=10.1073/pnas.1016106108;
RA Gobler C.J., Berry D.L., Dyhrman S.T., Wilhelm S.W., Salamov A.,
RA Lobanov A.V., Zhang Y., Collier J.L., Wurch L.L., Kustka A.B., Dill B.D.,
RA Shah M., VerBerkmoes N.C., Kuo A., Terry A., Pangilinan J., Lindquist E.A.,
RA Lucas S., Paulsen I.T., Hattenrath-Lehmann T.K., Talmage S.C., Walker E.A.,
RA Koch F., Burson A.M., Marcoval M.A., Tang Y.Z., Lecleir G.R., Coyne K.J.,
RA Berg G.M., Bertrand E.M., Saito M.A., Gladyshev V.N., Grigoriev I.V.;
RT "Niche of harmful alga Aureococcus anophagefferens revealed through
RT ecogenomics.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:4352-4357(2011).
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|RuleBase:RU003423};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317, ECO:0000256|RuleBase:RU003423}.
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DR EMBL; GL833123; EGB10928.1; -; Genomic_DNA.
DR RefSeq; XP_009034498.1; XM_009036250.1.
DR AlphaFoldDB; F0Y1J6; -.
DR EnsemblProtists; EGB10928; EGB10928; AURANDRAFT_58776.
DR GeneID; 20222660; -.
DR KEGG; aaf:AURANDRAFT_58776; -.
DR eggNOG; KOG0557; Eukaryota.
DR InParanoid; F0Y1J6; -.
DR OMA; RAMAQNM; -.
DR OrthoDB; 1399at2759; -.
DR Proteomes; UP000002729; Unassembled WGS sequence.
DR GO; GO:0045254; C:pyruvate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IEA:InterPro.
DR CDD; cd06849; lipoyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 2.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR045257; E2/Pdx1.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR23151; DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED; 1.
DR PANTHER; PTHR23151:SF75; DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT 5 OF PYRUVATE DEHYDROGENASE COMPLEX, CHLOROPLASTIC; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF02817; E3_binding; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 2.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003423};
KW Lipoyl {ECO:0000256|RuleBase:RU003423};
KW Reference proteome {ECO:0000313|Proteomes:UP000002729};
KW Transferase {ECO:0000256|RuleBase:RU003423}.
FT DOMAIN 33..112
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 226..263
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
SQ SEQUENCE 506 AA; 50297 MW; 3ED404536773E5BB CRC64;
MLLLLVAGAA ALRAPSGLRC PRFTGATVLR SSTTEIMMPA LSSTMTSGRV VSWLKNVGDK
IEAGDPIIVV ESDKADMEVE SYDEGARRRY LAAVFVGEGE DADVGVPVGV LAETPEEAKA
FSGGPSGGGA AAAEPAAPAA PAAPAAPAGD APESVAVAAY GAPAAAAAPA AAAAAPAAAA
PAAAPAAAAK APAGDRVAAS GYAKKVAAGA GVDVAAVAGT GPGGRVVARD AAKLAKSKNL
DLAGVAGTGR FGRVTEDDVK KALGIAEPAK PKLVPAGGPA PPPAGVVDMT GMQKAIAKNM
EATLAVPVFR VSKTVRTDAF DALYQKLKPD GVTVSALLAK AVAGALVKTP LMNAKYEPGA
FSYNGDVNVA MAVALDGGLI TPTLRNADQL SLADLSAEWK SLVGKAKSGS LKPEEYTTGT
FTISNLGMFD VAQFDAILPP GQGAILAISS SKNVVVPMPG SLLGVGIEKQ MTVTVTCDHR
IISGADAAVF LKDFAAAVEN PATLTK
//
