ID F0YF17_AURAN Unreviewed; 992 AA.
AC F0YF17;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=subtilisin {ECO:0000256|ARBA:ARBA00023619};
DE EC=3.4.21.62 {ECO:0000256|ARBA:ARBA00023619};
GN ORFNames=AURANDRAFT_65750 {ECO:0000313|EMBL:EGB06349.1};
OS Aureococcus anophagefferens (Harmful bloom alga).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Pelagophyceae; Pelagomonadales;
OC Aureococcus.
OX NCBI_TaxID=44056 {ECO:0000313|Proteomes:UP000002729};
RN [1] {ECO:0000313|EMBL:EGB06349.1, ECO:0000313|Proteomes:UP000002729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP 1984 {ECO:0000313|Proteomes:UP000002729};
RX PubMed=21368207; DOI=10.1073/pnas.1016106108;
RA Gobler C.J., Berry D.L., Dyhrman S.T., Wilhelm S.W., Salamov A.,
RA Lobanov A.V., Zhang Y., Collier J.L., Wurch L.L., Kustka A.B., Dill B.D.,
RA Shah M., VerBerkmoes N.C., Kuo A., Terry A., Pangilinan J., Lindquist E.A.,
RA Lucas S., Paulsen I.T., Hattenrath-Lehmann T.K., Talmage S.C., Walker E.A.,
RA Koch F., Burson A.M., Marcoval M.A., Tang Y.Z., Lecleir G.R., Coyne K.J.,
RA Berg G.M., Bertrand E.M., Saito M.A., Gladyshev V.N., Grigoriev I.V.;
RT "Niche of harmful alga Aureococcus anophagefferens revealed through
RT ecogenomics.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:4352-4357(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of proteins with broad specificity for peptide
CC bonds, and a preference for a large uncharged residue in P1.
CC Hydrolyzes peptide amides.; EC=3.4.21.62;
CC Evidence={ECO:0000256|ARBA:ARBA00023529};
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000256|PROSITE-
CC ProRule:PRU01240, ECO:0000256|RuleBase:RU003355}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; GL833135; EGB06349.1; -; Genomic_DNA.
DR RefSeq; XP_009038929.1; XM_009040681.1.
DR AlphaFoldDB; F0YF17; -.
DR EnsemblProtists; EGB06349; EGB06349; AURANDRAFT_65750.
DR GeneID; 20225491; -.
DR KEGG; aaf:AURANDRAFT_65750; -.
DR eggNOG; KOG1153; Eukaryota.
DR InParanoid; F0YF17; -.
DR OrthoDB; 380531at2759; -.
DR Proteomes; UP000002729; Unassembled WGS sequence.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07473; Peptidases_S8_Subtilisin_like; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034204; PfSUB1-like_cat_dom.
DR PANTHER; PTHR43399; SUBTILISIN-RELATED; 1.
DR PANTHER; PTHR43399:SF4; TK-SUBTILISIN; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000002729};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 17..992
FT /note="subtilisin"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003260999"
FT DOMAIN 180..445
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT REGION 619..638
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 187
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 244
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 406
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 992 AA; 104159 MW; 2E61E55724331B1D CRC64;
MLPMLRVLAV AALASAMVPR TMERVVQTAK KEAAAGTLDG TVKMKLKRSA GAEAPKVAAR
VAEAMGCAPP RRVFRPAGKH ELKHVRAGLD LHYSTDCAAA KEAGAPGTSA KPKAGRRAVW
ALESLARTDR ALLDAVETLA AGLAIQRTGY DMDDPLRSAQ THYDAINLDE AWEMGAGDPS
IVVAVVDTGM DMDHPDLQKN VWVNDGEICG NGVDDDKNGY VDDCYGYNFA DDKGNGDLFG
DGSHGTHCAG TIAADNDNDL YVSGVAGGKN GNAGASLMTL VAFGQVSYGG FAEGIVYAAD
NGAKIVSNSW GYTAPDQYAE DELAAIDYAD DLGVAVVFAA GNAGTDENWY PAYYDRAIAV
AAVDDDGVAA YFTCFGDWID VSAPGVGVLS TVAMSEGATD YYDGTSMACP HVAGLLALGW
AIDPSRSKDD VLDCLYGTAT DLDDVNGAYA GKLGAGLIAA GDFAACMKNG APSAAPTLSL
PPTRYPTTAS PTATFSPTDY GCGRCDLVLT LVLVTDQYPE ETGWSLSWDN PGGCRGDATA
RKSAGAYGAQ YTKYTETVEG LCAGFEYTFT IADTYGDGIL EGGSYVVTVG GADVAAGATF
QYSESTVFVA QYGGTPAPSA TPAPTYERTP APTTKPVTPL PTSDMGDCVV DYPDYVGDGY
CDAYGGGYNT EACGWDGGDC CFCTCDDGGS SYACGPYDCR DPDEAEPSCE VDDGAYAYGG
YGNYGYGSPY GNYGNYGYGA YGYDYDYWWY GEDDYGGDDM APTPSSGYVA GLAAWGQTAE
AVVVSWESDV WQESAYSFKV GVAREGGDMS WSYPACQASG QGAFFHVLTA ADGLECGESY
EILVMQGDAQ TETIVARTLA CDGSDDDAAC VCDCGSDYEE DDCADSTSWY YKKSKDTCSD
YVTKKSKNCK KKDEFKVKAE DASAAASLRA RACPLTCGVC SSSDCEDSTS WYYKKSKDTC
ADYVTKKSKN CKKYGVDDVL AEDACPVTCG AC
//
