ID F1LM43_RAT Unreviewed; 1225 AA.
AC F1LM43;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 25-MAY-2022, sequence version 4.
DT 27-MAR-2024, entry version 91.
DE SubName: Full=RAS protein-specific guanine nucleotide-releasing factor 1 {ECO:0000313|Ensembl:ENSRNOP00000018794.8};
GN Name=Rasgrf1 {ECO:0000313|Ensembl:ENSRNOP00000018794.8,
GN ECO:0000313|RGD:620395};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000313|Ensembl:ENSRNOP00000018794.8, ECO:0000313|Proteomes:UP000002494};
RN [1] {ECO:0000313|Ensembl:ENSRNOP00000018794.8, ECO:0000313|Proteomes:UP000002494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000018794.8,
RC ECO:0000313|Proteomes:UP000002494};
RX PubMed=15057822; DOI=10.1038/nature02426;
RG Rat Genome Sequencing Project Consortium;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2] {ECO:0000313|Ensembl:ENSRNOP00000018794.8}
RP IDENTIFICATION.
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000018794.8};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR AlphaFoldDB; F1LM43; -.
DR Ensembl; ENSRNOT00000018794.9; ENSRNOP00000018794.8; ENSRNOG00000014025.9.
DR RGD; 620395; Rasgrf1.
DR VEuPathDB; HostDB:ENSRNOG00000014025; -.
DR GeneTree; ENSGT00940000157599; -.
DR HOGENOM; CLU_003405_0_1_1; -.
DR OMA; SCNTNGY; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000014025; Expressed in frontal cortex and 2 other cell types or tissues.
DR ExpressionAtlas; F1LM43; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0035254; F:glutamate receptor binding; IEA:Ensembl.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0031175; P:neuron projection development; IEA:Ensembl.
DR GO; GO:0048168; P:regulation of neuronal synaptic plasticity; IEA:Ensembl.
DR GO; GO:0035020; P:regulation of Rac protein signal transduction; IEA:Ensembl.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IEA:Ensembl.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR GO; GO:0044342; P:type B pancreatic cell proliferation; IEA:Ensembl.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 2.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF193; RAS-SPECIFIC GUANINE NUCLEOTIDE-RELEASING FACTOR 1; 1.
DR Pfam; PF00169; PH; 2.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00233; PH; 2.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 2.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 2.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 4: Predicted;
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Reference proteome {ECO:0000313|Proteomes:UP000002494}.
FT DOMAIN 22..129
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 221..407
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 437..563
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 610..724
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 990..1222
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 688..711
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 781..821
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1225 AA; 140052 MW; 117F011F03B3F4D8 CRC64;
MQKAIRLNDG HVVSLGLLAQ RDGTRKGYLS KRSSDNPKWQ TKWFALLQNL LFYFESDSSS
RPSGLYLLEG SICKRMPSPK RGTSSKESDK QHHYFTVNFS NDSQKSLELR TDDSKDCDEW
VAAIARARYV SKFLQVQSSE RAGGKGGEGS IDPLKASLIA NLIKDNERIQ SNQLVAPEDE
DSDIKKIKKV QSFLRGWLCR RKWKNIIQDY IRSPHADSMR KRNQVVFSML EAEAEYVQQL
HILVNNFLRP LRMAASSKKP PITHDDVSSI FLNSETIMFL HQIFYQGLKA RIASWPTLVL
ADLFDILLPM LNIYQEFVRN HQYSLQILAH CKQNRDFDKL LKQYEAKPDC EERTLETFLT
YPMFQIPRYI LTLHELLAHT PHEHVERNSL DYAKSKLEEL SRVMHDEVSE TENIRKNLAI
ERMITEGCEI LLDTSQTFVR QGSLIQVPMS EKGKINKGRL GSLSLKKEGE RQCFLFSKHL
IICTRGSGSK LHLTKNGVIS LIDCTLLDDP ENMDDDGKGQ EVDHLDFKIW VEPKDSPPFT
VILVASSRQE KAAWTSDIIQ CVDNIRCNGL MMNAFEENSK VTVPQMIKSD ASLYCDDVDI
RFSKTMNSCK VLQIRYASVE RLLERLTDLR FLSIDFLNTF LHSYRVFTDA VVVLDKLISI
YKKPITAIPA RSLELLFSSS HNTKLLYGDA PKSPRASRKF SSPPPLAIGT SSPVRRRKLS
LNIPIITGGK ALELASLGCP SDGYTNIHSP ISPFGKTTLD TSKLCVASSL TRTPEEIDMT
TLEESSGFRK PTSDILKEES DDDQSDVDDT EVSPPTPKSF RNRITQEFPL FNYNSGIMMT
CRDLMDSNRS PLSATSAFAI ATAGANESPA NKEIYRRMSL ANTGYSSDQR NIDKEFVIRR
AATNRVLNVL RHWVTKHSQD FETDDLLKYK VICFLEEVMH DPDLLPQERK AAANIMRTLT
QEEITENHSM LDELLLMTEG VKTEPFENHS AMEIAEQLTL LDHLVFKSIP YEEFFGQGWM
KADKNERTPY IMKTTRHFNH ISNLIASEIL RNEEVSARAS TIEKWVAVAD ICRCLHNYNA
VLEITSSINR SAIFRLKKTW LKVSKQTKSL FDKLQKLVSS DGRFKNLRET LRNCDPPCVP
YLGMYLTDLA FLEEGTPNYT EDGLVNFSKM RMISHIIREI RQFQQTTYKI EPQPKVTQYL
VDETFVLDDE SLYEASLRIE PKLPT
//
