GenomeNet

Database: UniProt
Entry: F1Q101_CANLF
LinkDB: F1Q101_CANLF
Original site: F1Q101_CANLF 
ID   F1Q101_CANLF            Unreviewed;      2312 AA.
AC   F1Q101;
DT   03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT   31-OCT-2012, sequence version 2.
DT   05-JUN-2019, entry version 68.
DE   RecName: Full=Phosphoinositide phospholipase C {ECO:0000256|RuleBase:RU361133};
DE            EC=3.1.4.11 {ECO:0000256|RuleBase:RU361133};
GN   Name=PLCE1 {ECO:0000313|Ensembl:ENSCAFP00000011841,
GN   ECO:0000313|VGNC:VGNC:44650};
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae;
OC   Canis.
OX   NCBI_TaxID=9615 {ECO:0000313|Ensembl:ENSCAFP00000011841, ECO:0000313|Proteomes:UP000002254};
RN   [1] {ECO:0000313|Ensembl:ENSCAFP00000011841, ECO:0000313|Proteomes:UP000002254}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Boxer {ECO:0000313|Ensembl:ENSCAFP00000011841,
RC   ECO:0000313|Proteomes:UP000002254};
RX   PubMed=16341006; DOI=10.1038/nature04338;
RG   Broad Sequencing Platform;
RA   Lindblad-Toh K., Wade C.M., Mikkelsen T.S., Karlsson E.K., Jaffe D.B.,
RA   Kamal M., Clamp M., Chang J.L., Kulbokas E.J. III, Zody M.C.,
RA   Mauceli E., Xie X., Breen M., Wayne R.K., Ostrander E.A.,
RA   Ponting C.P., Galibert F., Smith D.R., deJong P.J., Kirkness E.F.,
RA   Alvarez P., Biagi T., Brockman W., Butler J., Chin C.-W., Cook A.,
RA   Cuff J., Daly M.J., DeCaprio D., Gnerre S., Grabherr M., Kellis M.,
RA   Kleber M., Bardeleben C., Goodstadt L., Heger A., Hitte C., Kim L.,
RA   Koepfli K.-P., Parker H.G., Pollinger J.P., Searle S.M.J.,
RA   Sutter N.B., Thomas R., Webber C., Baldwin J., Abebe A.,
RA   Abouelleil A., Aftuck L., Ait-Zahra M., Aldredge T., Allen N., An P.,
RA   Anderson S., Antoine C., Arachchi H., Aslam A., Ayotte L.,
RA   Bachantsang P., Barry A., Bayul T., Benamara M., Berlin A.,
RA   Bessette D., Blitshteyn B., Bloom T., Blye J., Boguslavskiy L.,
RA   Bonnet C., Boukhgalter B., Brown A., Cahill P., Calixte N.,
RA   Camarata J., Cheshatsang Y., Chu J., Citroen M., Collymore A.,
RA   Cooke P., Dawoe T., Daza R., Decktor K., DeGray S., Dhargay N.,
RA   Dooley K., Dooley K., Dorje P., Dorjee K., Dorris L., Duffey N.,
RA   Dupes A., Egbiremolen O., Elong R., Falk J., Farina A., Faro S.,
RA   Ferguson D., Ferreira P., Fisher S., FitzGerald M., Foley K.,
RA   Foley C., Franke A., Friedrich D., Gage D., Garber M., Gearin G.,
RA   Giannoukos G., Goode T., Goyette A., Graham J., Grandbois E.,
RA   Gyaltsen K., Hafez N., Hagopian D., Hagos B., Hall J., Healy C.,
RA   Hegarty R., Honan T., Horn A., Houde N., Hughes L., Hunnicutt L.,
RA   Husby M., Jester B., Jones C., Kamat A., Kanga B., Kells C.,
RA   Khazanovich D., Kieu A.C., Kisner P., Kumar M., Lance K., Landers T.,
RA   Lara M., Lee W., Leger J.-P., Lennon N., Leuper L., LeVine S., Liu J.,
RA   Liu X., Lokyitsang Y., Lokyitsang T., Lui A., Macdonald J., Major J.,
RA   Marabella R., Maru K., Matthews C., McDonough S., Mehta T.,
RA   Meldrim J., Melnikov A., Meneus L., Mihalev A., Mihova T., Miller K.,
RA   Mittelman R., Mlenga V., Mulrain L., Munson G., Navidi A., Naylor J.,
RA   Nguyen T., Nguyen N., Nguyen C., Nguyen T., Nicol R., Norbu N.,
RA   Norbu C., Novod N., Nyima T., Olandt P., O'Neill B., O'Neill K.,
RA   Osman S., Oyono L., Patti C., Perrin D., Phunkhang P., Pierre F.,
RA   Priest M., Rachupka A., Raghuraman S., Rameau R., Ray V., Raymond C.,
RA   Rege F., Rise C., Rogers J., Rogov P., Sahalie J., Settipalli S.,
RA   Sharpe T., Shea T., Sheehan M., Sherpa N., Shi J., Shih D., Sloan J.,
RA   Smith C., Sparrow T., Stalker J., Stange-Thomann N., Stavropoulos S.,
RA   Stone C., Stone S., Sykes S., Tchuinga P., Tenzing P., Tesfaye S.,
RA   Thoulutsang D., Thoulutsang Y., Topham K., Topping I., Tsamla T.,
RA   Vassiliev H., Venkataraman V., Vo A., Wangchuk T., Wangdi T.,
RA   Weiand M., Wilkinson J., Wilson A., Yadav S., Yang S., Yang X.,
RA   Young G., Yu Q., Zainoun J., Zembek L., Zimmer A., Lander E.S.;
RT   "Genome sequence, comparative analysis and haplotype structure of the
RT   domestic dog.";
RL   Nature 438:803-819(2005).
RN   [2] {ECO:0000313|Ensembl:ENSCAFP00000011841}
RP   IDENTIFICATION.
RC   STRAIN=Boxer {ECO:0000313|Ensembl:ENSCAFP00000011841};
RG   Ensembl;
RL   Submitted (JUL-2011) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-
CC         bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a
CC         1,2-diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:33179,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815,
CC         ChEBI:CHEBI:58456, ChEBI:CHEBI:203600; EC=3.1.4.11;
CC         Evidence={ECO:0000256|RuleBase:RU361133};
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; AAEX03015425; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AAEX03015426; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AAEX03015427; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   STRING; 9612.ENSCAFP00000011841; -.
DR   PaxDb; F1Q101; -.
DR   Ensembl; ENSCAFT00000012791; ENSCAFP00000011841; ENSCAFG00000007985.
DR   VGNC; VGNC:44650; PLCE1.
DR   eggNOG; KOG0169; Eukaryota.
DR   eggNOG; ENOG410XPSW; LUCA.
DR   GeneTree; ENSGT00940000157356; -.
DR   InParanoid; F1Q101; -.
DR   OMA; IRADVHQ; -.
DR   TreeFam; TF314432; -.
DR   Reactome; R-CFA-1855204; Synthesis of IP3 and IP4 in the cytosol.
DR   Proteomes; UP000002254; Chromosome 28.
DR   Bgee; ENSCAFG00000007985; Expressed in 3 organ(s), highest expression level in liver.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; IBA:GO_Central.
DR   GO; GO:0017016; F:Ras GTPase binding; IBA:GO_Central.
DR   GO; GO:0000187; P:activation of MAPK activity; IEA:Ensembl.
DR   GO; GO:0032835; P:glomerulus development; IEA:Ensembl.
DR   GO; GO:0032959; P:inositol trisphosphate biosynthetic process; IBA:GO_Central.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR   GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR   GO; GO:0046578; P:regulation of Ras protein signal transduction; IEA:Ensembl.
DR   GO; GO:0051209; P:release of sequestered calcium ion into cytosol; IBA:GO_Central.
DR   Gene3D; 2.60.40.150; -; 1.
DR   Gene3D; 3.20.20.190; -; 2.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR001192; PI-PLC_fam.
DR   InterPro; IPR028398; PLC-epsilon1.
DR   InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR   InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR   InterPro; IPR001711; PLipase_C_Pinositol-sp_Y.
DR   InterPro; IPR000159; RA_dom.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR10336; PTHR10336; 1.
DR   PANTHER; PTHR10336:SF6; PTHR10336:SF6; 1.
DR   Pfam; PF00168; C2; 1.
DR   Pfam; PF00388; PI-PLC-X; 1.
DR   Pfam; PF00387; PI-PLC-Y; 1.
DR   Pfam; PF00788; RA; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   PRINTS; PR00390; PHPHLIPASEC.
DR   SMART; SM00239; C2; 1.
DR   SMART; SM00148; PLCXc; 1.
DR   SMART; SM00149; PLCYc; 1.
DR   SMART; SM00314; RA; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF51695; SSF51695; 1.
DR   SUPFAM; SSF54236; SSF54236; 2.
DR   PROSITE; PS50004; C2; 1.
DR   PROSITE; PS50008; PIPLC_Y_DOMAIN; 1.
DR   PROSITE; PS50200; RA; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
PE   4: Predicted;
KW   Complete proteome {ECO:0000313|Proteomes:UP000002254};
KW   Guanine-nucleotide releasing factor {ECO:0000256|PROSITE-
KW   ProRule:PRU00168}; Hydrolase {ECO:0000256|RuleBase:RU361133};
KW   Lipid degradation {ECO:0000256|RuleBase:RU361133};
KW   Lipid metabolism {ECO:0000256|RuleBase:RU361133};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002254};
KW   Transducer {ECO:0000256|SAAS:SAAS01019513}.
FT   DOMAIN      540    794       Ras-GEF. {ECO:0000259|PROSITE:PS50009}.
FT   DOMAIN     1767   1857       PI-PLC Y-box. {ECO:0000259|PROSITE:
FT                                PS50008}.
FT   DOMAIN     1872   1956       C2. {ECO:0000259|PROSITE:PS50004}.
FT   DOMAIN     2147   2249       Ras-associating. {ECO:0000259|PROSITE:
FT                                PS50200}.
FT   REGION       22     49       Disordered. {ECO:0000256|MobiDB-lite:
FT                                F1Q101}.
FT   REGION     1062   1202       Disordered. {ECO:0000256|MobiDB-lite:
FT                                F1Q101}.
FT   REGION     1576   1601       Disordered. {ECO:0000256|MobiDB-lite:
FT                                F1Q101}.
FT   REGION     1693   1734       Disordered. {ECO:0000256|MobiDB-lite:
FT                                F1Q101}.
FT   REGION     2272   2312       Disordered. {ECO:0000256|MobiDB-lite:
FT                                F1Q101}.
FT   COMPBIAS   1066   1094       Polar. {ECO:0000256|MobiDB-lite:F1Q101}.
FT   COMPBIAS   1098   1135       Polyampholyte. {ECO:0000256|MobiDB-lite:
FT                                F1Q101}.
FT   COMPBIAS   1140   1202       Polar. {ECO:0000256|MobiDB-lite:F1Q101}.
FT   COMPBIAS   1714   1734       Polar. {ECO:0000256|MobiDB-lite:F1Q101}.
SQ   SEQUENCE   2312 AA;  259201 MW;  DC8EF0895B996C9C CRC64;
     MTSEGMAASV LTPVTQRKVT FAPSAVDESS EKVSDISVPK AHSVKQSEQT STIPWMNKLK
     EESSGSNLPK ILSIAREKIA SDENSNEECW TESTPVSVKN LNINHNNILT NRQCVLPQSQ
     SYDTCNSVME EDPCLETGIS SSLERKVFPG IQLEVNRPPM DFRPPGLMDF STLGSQSAIV
     DTGQAHPDSN KAAFQIFNYK VDRRMSDTFC TLSGDLILDD CGNCVPLSSG FGGEQKKNYV
     AYTCKLMELA ENCDNENGQL QCDDYDALDD KYLCFEDSCQ RDSVVCSSDS FRREDLTNSP
     PAKTFLSHFE DFPDNGEDVE DFLKNKKERS TLLVRRFCKN DREVKKSVYT GTRAIMRTLP
     SGHIGLEAYS YIDQKRSGPL LPRGRVLEQL PVVAIRQDGS QCLSEAQWYR IYNAVRREEE
     IENTIGSLLH YFTKLPASKT AHERISVGPC LKQCVRDTIC EYRATLQRTS ISQYITGSLL
     EATTSLGARS GLLSTFGGST GRMMLKERQP GTSMANSSAL PSSSAGISKE LIDLQPLIQF
     PEEVASILME QEQNIYRRVL PVDYLYFLTR DLGTPECQTP LPCLKASISA SILSSQNGEH
     NALEDLVMRF NEKVAAWLSW LTPTLAKMEK KREVFSYLVH VAKCCWNMGN YNAVMEFLAG
     LRSRKVLKMW QFMDQSDLET MRSLKDAMAQ HESSCEYRKV ITRALHIPGC KVVPFCGVFL
     KELCEVLDGA SGLMKLCPRY NSQEETLEFV ADYSGQDNFL QRVGHNGLKN SEKESTVNSI
     FQIIRSCSRS LEAEEEDSPS EGNSSRKNSL RDKARWQFII GDLLDSENDI FEQSKEWDSP
     SSEEPQKAFD HGTELIPWYV LSIRADVHQF LLQGATVLRY DQDTHLSARC FLQLQPDNST
     LTWIKPTAAS PASARAKLGV LSNTSEPGKF PSPGSAGLSG LAEGVLDLFS AKAVYMGHPS
     IDIHTVCVQN KLGSMFLSET GVTLLYGLQT TDNRLLHFVA PKHTAKMLFS GLLELTRAVR
     KMRKFPDQRQ QWLRKQYVSL YQEDGRYEGP TLAHAVELFG GRRWSTRNPS PGTSAKSAEK
     PSVQRNNTLG ISTTKKKKKI LIRGESGEAA DDEMATRKAK MHRECRSRSG SDPQDMNEQE
     ESEANAIMSP PNTLPSRRAH SLTTAGSPNL SAGTSSPIRP VSSPVLSSSN KSPSSAWSSS
     SWHGRIKGGM KGFQSFMVSD SNMSFVEFVE LFKSFSVRSR KDLKDLFDIY AVPCNRAGSE
     SAPLYTNLTI DENTSDLQPD LDLLTRNVSD LGLFIKSKQQ LSDNQRQISD AIAAASIVTN
     GTGIESTSLG IFGVGILQLN DFLVNCQGEH CTYDEILSII QKFEPSISMC HQGLLSFEGF
     ARFLMDKDNF ASKNDESQEN IKDLQLPLSY YYIESSHNTY LTGHQLKGES SVELYSQVLL
     QGCRSVELDC WDGDDGMPII YHGHTLTTKI PFKEVVEAID RSAFINSDLP IIISIENHCS
     LPQQRKMAEI FKTVFGEKLV AKFLFESDFS DDPMLPSPDQ LRRKVLLKNK KLKAHQTPVD
     ILKQKAHQLA SMQAQAYNGG NVNPPPANNE EEEDEEDEYD YDYESLSDDN ILEDRPENKS
     CNDKLQFEYN EEIPKRIKKA DNSAFNKGKV YDMELGEEFY LPQNKKESRQ IAPELSDLVI
     YCQAVKFPGT GLSTLNASGS NRGKERKSRK SIFGNNPGRM SPGETASFNK ASGKSSCEGM
     RQAWEESSSP LNPTTSLSAI IRTPKCYHIS SLNENAAKRL CRRYSQKLIQ HTACQLLRTY
     PAATRIDSSN PNPLLFWLHG IQLVALNYQT DDLPLHLNAA MFEANGGCGY VLKPPVLWDK
     NCPMYQKFSP LERDLDNMEP AVYSLTIVSG QNVCPSSSTG SPCIEVDVLG MPLDSCHFRT
     KPIHRNTLNP MWNEQFLFRV HFEDLIFLRF AVVENNSSAI TAQRIIPLKA LKRGYRHLQL
     RNLHNEVLEI SSLFINSRRM EENSSSSATP ASLMFNTEER KCLQTHRVTV HGVPGPEPFT
     VFSISGGTKA KQLLQQILTT EQDTKPIVTD YFLMEEKYFI SKEKNECRKQ PFQRVIGPEE
     EIMQILSSWF PEEGYVGRIV LKTQQENLEE RSIVQDEKEV ILSSEEESFF VQVHDVSPEQ
     PRTVIKAPRV STAQDVIQQT LCKAKYSYSI LSNPNPSDYV LLEEVVKDAT KKSSTPKSSQ
     RVLLDQECVF QAQSKWKGAG KFILRLKEQV QASREDKRKG ISFASEFKKF TKSTKQPRGL
     TSSQVLASES VQNKEEKPTG SLSSSDTTDS RQ
//
DBGET integrated database retrieval system