ID F1QKS8_DANRE Unreviewed; 156 AA.
AC F1QKS8; A0A8M1PDY6;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=Arachidonate 5-lipoxygenase-activating protein {ECO:0000256|ARBA:ARBA00040386};
GN Name=alox5ap {ECO:0000313|Ensembl:ENSDARP00000071476,
GN ECO:0000313|RefSeq:NP_956355.2,
GN ECO:0000313|ZFIN:ZDB-GENE-030131-9322};
GN Synonyms=wu:fj65d02 {ECO:0000313|RefSeq:NP_956355.2}, zgc:63982
GN {ECO:0000313|RefSeq:NP_956355.2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000071476};
RN [1] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=19389629;
RA Kalen M., Wallgard E., Asker N., Nasevicius A., Athley E., Billgren E.,
RA Larson J.D., Wadman S.A., Norseng E., Clark K.J., He L.,
RA Karlsson-Lindahl L., Hager A.K., Weber H., Augustin H., Samuelsson T.,
RA Kemmet C.K., Utesch C.M., Essner J.J., Hackett P.B., Hellstrom M.;
RT "Combination of reverse and chemical genetic screens reveals angiogenesis
RT inhibitors and targets.";
RL Chem. Biol. 16:432-441(2009).
RN [2] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=20424185; DOI=10.1182/blood-2010-01-262873;
RA Zakrzewska A., Cui C., Stockhammer O.W., Benard E.L., Spaink H.P.,
RA Meijer A.H.;
RT "Macrophage-specific gene functions in Spi1-directed innate immunity.";
RL Blood 116:E1-E11(2010).
RN [3] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=21687630; DOI=10.1371/journal.pone.0020974;
RA Li I.C., Chan C.T., Lu Y.F., Wu Y.T., Chen Y.C., Li G.B., Lin C.Y.,
RA Hwang S.P.;
RT "Zebrafish kruppel-like factor 4a represses intestinal cell proliferation
RT and promotes differentiation of intestinal cell lineages.";
RL PLoS ONE 6:e20974-e20974(2011).
RN [4] {ECO:0000313|Ensembl:ENSDARP00000071476}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000071476};
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
RN [5] {ECO:0000313|Ensembl:ENSDARP00000071476, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000071476};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [6] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=25461745;
RA Glisic B., Mihaljevic I., Popovic M., Zaja R., Loncar J., Fent K.,
RA Kovacevic R., Smital T.;
RT "Characterization of glutathione-S-transferases in zebrafish (Danio
RT rerio).";
RL Aquat. Toxicol. 158:50-62(2015).
RN [7] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=25573385;
RA Knight O.M., Van Der Kraak G.;
RT "The role of eicosanoids in 17alpha, 20beta-dihydroxy-4-pregnen-3-one-
RT induced ovulation and spawning in Danio rerio.";
RL Gen. Comp. Endocrinol. 213:50-58(2015).
RN [8] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=27219276;
RA Chatzopoulou A., Heijmans J.P., Burgerhout E., Oskam N., Spaink H.P.,
RA Meijer A.H., Schaaf M.J.;
RT "Glucocorticoid-Induced Attenuation of the Inflammatory Response in
RT Zebrafish.";
RL Endocrinology 157:2772-2784(2016).
RN [9] {ECO:0000313|RefSeq:NP_956355.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RX PubMed=30765415; DOI=10.1242/dmm.037762;
RA Kuil L.E., Oosterhof N., Geurts S.N., van der Linde H.C., Meijering E.,
RA van Ham T.J.;
RT "Reverse genetic screen reveals that Il34 facilitates yolk sac macrophage
RT distribution and seeding of the brain. .";
RL Dis. Model. Mech. 12:dmm037762-dmm037762(2019).
RN [10] {ECO:0000313|RefSeq:NP_956355.2}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_956355.2};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Required for leukotriene biosynthesis by ALOX5 (5-
CC lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid,
CC and could play an essential role in the transfer of arachidonic acid to
CC ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of
CC leukotrienes. {ECO:0000256|ARBA:ARBA00037558}.
CC -!- SUBUNIT: Homotrimer. Interacts with LTC4S and ALOX5.
CC {ECO:0000256|ARBA:ARBA00038708}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}. Nucleus membrane
CC {ECO:0000256|ARBA:ARBA00004232}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004232}.
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DR EMBL; BX548163; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_956355.2; NM_200061.2.
DR STRING; 7955.ENSDARP00000071476; -.
DR PaxDb; 7955-ENSDARP00000071476; -.
DR Ensembl; ENSDART00000077008; ENSDARP00000071476; ENSDARG00000054755.
DR Ensembl; ENSDART00000077008.7; ENSDARP00000071476.5; ENSDARG00000054755.7.
DR GeneID; 337376; -.
DR KEGG; dre:337376; -.
DR AGR; ZFIN:ZDB-GENE-030131-9322; -.
DR ZFIN; ZDB-GENE-030131-9322; alox5ap.
DR eggNOG; ENOG502RZJB; Eukaryota.
DR HOGENOM; CLU_110291_0_0_1; -.
DR OMA; HNNTSAF; -.
DR OrthoDB; 5343039at2759; -.
DR PhylomeDB; F1QKS8; -.
DR TreeFam; TF105328; -.
DR Reactome; R-DRE-2142688; Synthesis of 5-eicosatetraenoic acids.
DR Reactome; R-DRE-2142691; Synthesis of Leukotrienes (LT) and Eoxins (EX).
DR Reactome; R-DRE-2142700; Synthesis of Lipoxins (LX).
DR Proteomes; UP000000437; Alternate scaffold 10.
DR Proteomes; UP000000437; Chromosome 10.
DR Bgee; ENSDARG00000054755; Expressed in granulocyte and 15 other cell types or tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR GO; GO:0004602; F:glutathione peroxidase activity; IBA:GO_Central.
DR GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR GO; GO:0004464; F:leukotriene-C4 synthase activity; IBA:GO_Central.
DR GO; GO:0001525; P:angiogenesis; IMP:ZFIN.
DR GO; GO:0019370; P:leukotriene biosynthetic process; IBA:GO_Central.
DR Gene3D; 1.20.120.550; Membrane associated eicosanoid/glutathione metabolism-like domain; 1.
DR InterPro; IPR001446; 5_LipOase_AP.
DR InterPro; IPR023352; MAPEG-like_dom_sf.
DR InterPro; IPR001129; Membr-assoc_MAPEG.
DR PANTHER; PTHR10250:SF2; ARACHIDONATE 5-LIPOXYGENASE-ACTIVATING PROTEIN; 1.
DR PANTHER; PTHR10250; MICROSOMAL GLUTATHIONE S-TRANSFERASE; 1.
DR Pfam; PF01124; MAPEG; 1.
DR PRINTS; PR00488; 5LPOXGNASEAP.
DR SUPFAM; SSF161084; MAPEG domain-like; 1.
PE 4: Predicted;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Leukotriene biosynthesis {ECO:0000256|ARBA:ARBA00022751};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Signal {ECO:0000256|SAM:SignalP, ECO:0000313|RefSeq:NP_956355.2};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..156
FT /note="Arachidonate 5-lipoxygenase-activating protein"
FT /evidence="ECO:0000256|SAM:SignalP,
FT ECO:0000313|RefSeq:NP_956355.2"
FT /id="PRO_5035035881"
FT TRANSMEM 62..88
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 113..131
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 156 AA; 17365 MW; F670201217383CC6 CRC64;
MYASVMDNIF LLVLVTLLSV VQNVFFALKV EKECTGHQSK RSAAFERLSC AKRNCMDTYP
TFLAVLWCAG ICLSQAPAAF AGILYLVVRQ KYFVGYLGET CQSTPGFLFG KRILFFLSLM
CVVGIINHLM LTYGGSDYKE YIQTITKAAS TLLLLP
//
