ID F1REP3_DANRE Unreviewed; 280 AA.
AC F1REP3; A0A8M1PHD0;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE RecName: Full=Metalloendopeptidase {ECO:0000256|RuleBase:RU361183};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU361183};
GN Name=npsn {ECO:0000313|Ensembl:ENSDARP00000100461,
GN ECO:0000313|RefSeq:NP_991319.2, ECO:0000313|ZFIN:ZDB-GENE-040420-2};
GN Synonyms=npsnl {ECO:0000313|RefSeq:NP_991319.2}, zgc:110470
GN {ECO:0000313|RefSeq:NP_991319.2}, zgc:152831
GN {ECO:0000313|RefSeq:NP_991319.2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000100461};
RN [1] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.D., Sun X.J., Deng M., Zhang G.W., Zhou Y., Wu X.Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.L., Fan H.Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [2] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=17016731; DOI=10.1007/s00427-006-0104-5;
RA Kawaguchi M., Yasumasu S., Hiroi J., Naruse K., Inoue M., Iuchi I.;
RT "Evolution of teleostean hatching enzyme genes and their paralogous
RT genes.";
RL Dev. Genes Evol. 216:769-784(2006).
RN [3] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=18641354;
RA Zhang Y., Bai X.T., Zhu K.Y., Jin Y., Deng M., Le H.Y., Fu Y.F., Chen Y.,
RA Zhu J., Look A.T., Kanki J., Chen Z., Chen S.J., Liu T.X.;
RT "In vivo interstitial migration of primitive macrophages mediated by JNK-
RT matrix metalloproteinase 13 signaling in response to acute injury.";
RL J. Immunol. 181:2155-2164(2008).
RN [4] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=21901140; DOI=10.1371/journal.pone.0023845;
RA Keightley M.C., Layton J.E., Hayman J.W., Heath J.K., Lieschke G.J.;
RT "Mediator subunit 12 is required for neutrophil development in zebrafish.";
RL PLoS ONE 6:e23845-e23845(2011).
RN [5] {ECO:0000313|Ensembl:ENSDARP00000100461}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000100461};
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
RN [6] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=23714367;
RA Keightley M.C., Crowhurst M.O., Layton J.E., Beilharz T., Markmiller S.,
RA Varma S., Hogan B.M., de Jong-Curtain T.A., Heath J.K., Lieschke G.J.;
RT "In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects
RT transcript splicing, cell survival and myeloid differentiation.";
RL FEBS Lett. 587:2150-2157(2013).
RN [7] {ECO:0000313|Ensembl:ENSDARP00000100461, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000100461};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [8] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=26411530;
RA Murayama E., Sarris M., Redd M., Le Guyader D., Vivier C., Horsley W.,
RA Trede N., Herbomel P.;
RT "NACA deficiency reveals the crucial role of somite-derived stromal cells
RT in haematopoietic niche formation.";
RL Nat. Commun. 6:8375-8375(2015).
RN [9] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=26469318;
RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA Reith W., Hertzano R.;
RT "RFX transcription factors are essential for hearing in mice.";
RL Nat. Commun. 6:8549-8549(2015).
RN [10] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=27974754;
RA Lin Q., Fan S., Zhang Y., Xu M., Zhang H., Yang Y., Lee A.P.,
RA Woltering J.M., Ravi V., Gunter H.M., Luo W., Gao Z., Lim Z.W., Qin G.,
RA Schneider R.F., Wang X., Xiong P., Li G., Wang K., Min J., Zhang C.,
RA Qiu Y., Bai J., He W., Bian C., Zhang X., Shan D., Qu H., Sun Y., Gao Q.,
RA Huang L., Shi Q., Meyer A., Venkatesh B.;
RT "The seahorse genome and the evolution of its specialized morphology.";
RL Nature 540:395-399(2016).
RN [11] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=28835569; DOI=10.1098/rsob.170040;
RA Di Q., Lin Q., Huang Z., Chi Y., Chen X., Zhang W., Zhang Y.;
RT "Zebrafish nephrosin helps host defence against Escherichia coli infection.
RT .";
RL Open Biol. 7:170040-170040(2017).
RN [12] {ECO:0000313|RefSeq:NP_991319.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RX PubMed=29378332;
RA Yu S.H., Zhu K.Y., Zhang F., Wang J., Yuan H., Chen Y., Jin Y., Dong M.,
RA Wang L., Jia X.E., Gao L., Dong Z.W., Ren C.G., Chen L.T., Huang Q.H.,
RA Deng M., Zon L.I., Zhou Y., Zhu J., Xu P.F., Liu T.X.;
RT "The histone demethylase Jmjd3 regulates zebrafish myeloid development by
RT promoting spi1 expression.";
RL Biochim Biophys Acta Gene Regul Mech 1861:106-116(2018).
RN [13] {ECO:0000313|RefSeq:NP_991319.2}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_991319.2};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU01211,
CC ECO:0000256|RuleBase:RU361183};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PROSITE-
CC ProRule:PRU01211, ECO:0000256|RuleBase:RU361183};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01211}.
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DR EMBL; BX957297; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_991319.2; NM_205756.3.
DR STRING; 7955.ENSDARP00000100461; -.
DR PaxDb; 7955-ENSDARP00000100461; -.
DR Ensembl; ENSDART00000109463; ENSDARP00000100461; ENSDARG00000010423.
DR Ensembl; ENSDART00000109463.5; ENSDARP00000100461.2; ENSDARG00000010423.9.
DR GeneID; 404039; -.
DR AGR; ZFIN:ZDB-GENE-040420-2; -.
DR CTD; 404039; -.
DR ZFIN; ZDB-GENE-040420-2; npsn.
DR eggNOG; KOG3714; Eukaryota.
DR OMA; CTASGCM; -.
DR OrthoDB; 2876645at2759; -.
DR PhylomeDB; F1REP3; -.
DR Proteomes; UP000000437; Chromosome 7.
DR Bgee; ENSDARG00000010423; Expressed in granulocyte and 27 other cell types or tissues.
DR GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0042742; P:defense response to bacterium; IMP:ZFIN.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04283; ZnMc_hatching_enzyme; 1.
DR Gene3D; 3.40.390.10; Collagenase (Catalytic Domain); 1.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR001506; Peptidase_M12A.
DR InterPro; IPR006026; Peptidase_Metallo.
DR InterPro; IPR034039; ZnMP_hatching_enz.
DR PANTHER; PTHR10127; DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING; 1.
DR PANTHER; PTHR10127:SF779; METALLOENDOPEPTIDASE; 1.
DR Pfam; PF01400; Astacin; 1.
DR PRINTS; PR00480; ASTACIN.
DR SMART; SM00235; ZnMc; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
DR PROSITE; PS51864; ASTACIN; 1.
PE 1: Evidence at protein level;
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU01211,
KW ECO:0000256|RuleBase:RU361183};
KW Metal-binding {ECO:0000256|PROSITE-ProRule:PRU01211,
KW ECO:0000256|RuleBase:RU361183};
KW Metalloprotease {ECO:0000256|PROSITE-ProRule:PRU01211,
KW ECO:0000256|RuleBase:RU361183};
KW Protease {ECO:0000256|PROSITE-ProRule:PRU01211,
KW ECO:0000256|RuleBase:RU361183};
KW Proteomics identification {ECO:0007829|PeptideAtlas:F1REP3};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Signal {ECO:0000256|RuleBase:RU361183, ECO:0000313|RefSeq:NP_991319.2};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU01211, ECO:0000256|RuleBase:RU361183}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|RuleBase:RU361183"
FT CHAIN 20..280
FT /note="Metalloendopeptidase"
FT /evidence="ECO:0000256|RuleBase:RU361183,
FT ECO:0000313|RefSeq:NP_991319.2"
FT /id="PRO_5035035985"
FT DOMAIN 77..274
FT /note="Peptidase M12A"
FT /evidence="ECO:0000259|PROSITE:PS51864"
FT ACT_SITE 176
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
FT BINDING 175
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
FT BINDING 179
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
FT BINDING 185
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
SQ SEQUENCE 280 AA; 31433 MW; 14805674D89AE630 CRC64;
MYLLVVVISL LLSSGPVQSR PVEDLIEKMS KKTGGNFTKK GNLLVSSVIE TSKHAGENTD
GPVILFGDIA FPRGLQNADQ CTARGCKWDR SRDGLVYVPY QISRAYSPRE VAVIEQGLQS
FSAVSCIRFV PHTGERNYLN IKSESGCYSY LGRIGGGQVV SLQRQGCVYF STVQHELLHA
LGFHHEQNRS DRDNHIRILY QNIIPAQQYN FNKQNTNNLG TPYDYNSVMQ YSRYAFSMNN
QPTMVPVPNA NVVLGEAQSM SPNDILRINR LYCSTFNISY
//
