ID F1VZP8_9BURK Unreviewed; 238 AA.
AC F1VZP8;
DT 31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 31-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Dual-specificity RNA pseudouridine synthase RluF {ECO:0000256|ARBA:ARBA00039989};
DE EC=5.4.99.21 {ECO:0000256|ARBA:ARBA00038922};
DE AltName: Full=23S rRNA pseudouridine(2604) synthase {ECO:0000256|ARBA:ARBA00041697};
DE AltName: Full=Ribosomal large subunit pseudouridine synthase F {ECO:0000256|ARBA:ARBA00042890};
DE AltName: Full=rRNA pseudouridylate synthase F {ECO:0000256|ARBA:ARBA00042843};
DE AltName: Full=rRNA-uridine isomerase F {ECO:0000256|ARBA:ARBA00043147};
DE AltName: Full=tRNA(Tyr) pseudouridine(35) synthase {ECO:0000256|ARBA:ARBA00041420};
GN ORFNames=IMCC9480_3209 {ECO:0000313|EMBL:EGF31918.1};
OS Oxalobacteraceae bacterium IMCC9480.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae.
OX NCBI_TaxID=937450 {ECO:0000313|EMBL:EGF31918.1, ECO:0000313|Proteomes:UP000004494};
RN [1] {ECO:0000313|EMBL:EGF31918.1, ECO:0000313|Proteomes:UP000004494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IMCC9480 {ECO:0000313|EMBL:EGF31918.1,
RC ECO:0000313|Proteomes:UP000004494};
RX PubMed=21572000; DOI=10.1128/JB.05088-11;
RA Oh H.M., Lee K., Jang Y., Kang I., Kim H.J., Kang T.W., Kim S.Y., Cho J.C.;
RT "Genome sequence of strain IMCC9480, a xanthorhodopsin-bearing
RT betaproteobacterium isolated from the Arctic Ocean.";
RL J. Bacteriol. 193:3421-3421(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA;
CC Xref=Rhea:RHEA:38875, Rhea:RHEA-COMP:10093, Rhea:RHEA-COMP:10094,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.21;
CC Evidence={ECO:0000256|ARBA:ARBA00036535};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr);
CC Xref=Rhea:RHEA:60556, Rhea:RHEA-COMP:15607, Rhea:RHEA-COMP:15608,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|ARBA:ARBA00036390};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGF31918.1}.
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DR EMBL; AEPR01000325; EGF31918.1; -; Genomic_DNA.
DR AlphaFoldDB; F1VZP8; -.
DR Proteomes; UP000004494; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47683:SF2; S4 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS50889; S4; 1.
PE 4: Predicted;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000004494};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 2..39
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|Pfam:PF01479"
FT DOMAIN 57..201
FT /note="Pseudouridine synthase RsuA/RluA-like"
FT /evidence="ECO:0000259|Pfam:PF00849"
SQ SEQUENCE 238 AA; 26864 MW; 6BD909D7BC2BA6E5 CRC64;
MSELGLCSRR EADVWIEKGW VRVDGKVVSE LGSKVLPSQH VTIERQAAAE QSKLVTILIN
KPMGYVSGQA EDGYQPAVTL IKPENRWAED QATTQFHPTQ LRSLVPAGRL DIDSIGLLVL
TQDGRVAKQL IGETTEVEKE YLVRVQYDRP GRLPENQLLK LQHGLTMDGK ELLPAKVRWQ
NEDQLSFILR EGKKRQIRRM CDMVGLKVLG LKRVRIGNIK LGDLPVGEWR YLGADEGF
//
