ID F2U0G4_SALR5 Unreviewed; 2017 AA.
AC F2U0G4;
DT 31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 31-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=E3 ubiquitin-protein ligase TRIP12 {ECO:0000256|ARBA:ARBA00018006};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
DE AltName: Full=HECT-type E3 ubiquitin transferase TRIP12 {ECO:0000256|ARBA:ARBA00030738};
DE AltName: Full=Thyroid receptor-interacting protein 12 {ECO:0000256|ARBA:ARBA00032457};
GN ORFNames=PTSG_11742 {ECO:0000313|EMBL:EGD80892.1};
OS Salpingoeca rosetta (strain ATCC 50818 / BSB-021).
OC Eukaryota; Choanoflagellata; Craspedida; Salpingoecidae; Salpingoeca.
OX NCBI_TaxID=946362 {ECO:0000313|Proteomes:UP000007799};
RN [1] {ECO:0000313|Proteomes:UP000007799}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50818 {ECO:0000313|Proteomes:UP000007799};
RA Russ C., Cuomo C., Burger G., Gray M.W., Holland P.W.H., King N.,
RA Lang F.B.F., Roger A.J., Ruiz-Trillo I., Young S.K., Zeng Q., Gargeya S.,
RA Alvarado L., Berlin A., Chapman S.B., Chen Z., Freedman E., Gellesch M.,
RA Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C.,
RA Mehta T., Neiman D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., White J., Yandava C., Haas B., Nusbaum C.,
RA Birren B.;
RT "Annotation of Salpingoeca rosetta.";
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification. {ECO:0000256|ARBA:ARBA00043952}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the UPL family. K-HECT subfamily.
CC {ECO:0000256|ARBA:ARBA00006331}.
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DR EMBL; GL832958; EGD80892.1; -; Genomic_DNA.
DR RefSeq; XP_004997453.1; XM_004997396.1.
DR STRING; 946362.F2U0G4; -.
DR EnsemblProtists; EGD80892; EGD80892; PTSG_11742.
DR GeneID; 16078050; -.
DR KEGG; sre:PTSG_11742; -.
DR eggNOG; KOG0168; Eukaryota.
DR eggNOG; KOG0170; Eukaryota.
DR InParanoid; F2U0G4; -.
DR OMA; AEPLSQF; -.
DR OrthoDB; 1093891at2759; -.
DR Proteomes; UP000007799; Unassembled WGS sequence.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR Gene3D; 3.30.720.50; -; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 2.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR045322; HECTD1/TRIP12-like.
DR InterPro; IPR004170; WWE-dom.
DR InterPro; IPR018123; WWE-dom_subgr.
DR InterPro; IPR037197; WWE_dom_sf.
DR PANTHER; PTHR45670; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR PANTHER; PTHR45670:SF1; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF02825; WWE; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00678; WWE; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF117839; WWE domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50918; WWE; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000007799};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 566..642
FT /note="WWE"
FT /evidence="ECO:0000259|PROSITE:PS50918"
FT DOMAIN 1708..2017
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 461..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 781..847
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1061..1160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1172..1309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1358..1421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1623..1708
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..504
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 511..527
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 789..814
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1184..1201
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1216..1237
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1272..1288
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1358..1383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1623..1649
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1654..1683
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1684..1699
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1984
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 2017 AA; 215886 MW; 0471EE2D5EAAD353 CRC64;
MWRKPRAADV TISGSSSSSS SSNSGANSGN NSGSESVSSR TRSRTSTPLP PSGDAASDTL
PPPPPRRSSR RKAKKATQPS EAATSATTSK KSTSSKASSS SSRRNKGQAK RKAVATSEWI
EEDIDTADTA DTGTTTATTA DTATSSSSTR RSRQSGVSQR KRAKTQSFMA GLPFHQIFGA
PLMQQRGGSS GIVAGLKSGD FLQKEHALDT LNTKFLMEPA EQWRSLPFEE IIDALDAIIK
SMSPEDAHLA SRAARAASSL LEARPQTASI VAKLGPAIMF RFQQMDMDFT EAGLACLKRL
AREAPTEVLQ LNAVPTVTMM IDFFMGASQK DAIELISSAA DVCDASTFPQ HMREAVSGLT
QFLRMFAVRD PAVVEATCVL FFKLTRNLAA SPEHVEALAS DGLPAVFVQI LQTKPLPIAR
EGLTAVLASL ALMARSSTAT AVALVQDDAL LPMLKMALTD AKHRSRRAGS TRGADGTGAG
TAGADEDVGV DDEGDDDDDV AVVEGGAEDT ATGASAQQGQ GQEDEKQENQ GGAGIGMQLR
ANQLLHVVAL IRHLLPPLPT THPFHTFTRD VLPTDDEAVW EWRSGASTWR PYTTAATASI
ERAYATGAPS VTITVGSQTF VVDVQRMRQI NLSSHGVREV RRTERPAPPP PSGDALHKHY
AQHPAALSSM IVELIRPLFE LYLASARTDI HAEFAHTLVR MVHAATPPTL VESLRSVSMS
NYLAQMLHSR EPVVVMGALA TASCLLQRLP DIFAVHFRRK GVLHRVEQLC KMRVEVVAPT
PATPGASTAA AEGGSTPSTP AAATAATPAT PSDALSAPSP HAAMLSLQRR NTAAAREESR
NMRRRARKQR MMQAKKVAYD FAREAFGDVL ASTEADADID AIISAVKAAT SELRAATSPF
DQDKASAALT KLSDLCSGNA DGMTAFEFTH YGTAHALATF LTVEDDTTPH RLALFAHLFL
VHDAAKLHAL QRLLDLVHGA LNLNENFESS GPTKQQFERR NFTVRVSRAA DEKNLGSVDL
NLSVPSTVTV QALYDYVDKH LKQARTGPDR LREAHRLLRR HTEAQRRRRQ QQQQQEGGGG
DDGGEGTRRR SSRRRGQQQP ALGGADADAG GDGDEDTTGS DTTRTHARRL RRSARRHGAR
RGGSNGNDGG DEDAGLGSGD AASDLLSAIA ATLRSRGRAP GNDEDNEDED DDGDGDVNED
GLEAEEVLRY LLGGGGEDEE EEEADLEAQE EEGDEETPAD APQKVVVDDA GDDAEGGADA
DDDDGHGNAG DGGASTGSNA STRARLSRTS RSRASRLRHQ RLARSDSGQS VLRISAGDDW
WKVIAKGFRN KNALDLTLNG QVLPWNYSMI QAVRELTSAS RPASRSGSRT PGVDASSTGG
SDDMPTRVHV KYRKASARSR QVSVQSLRPD SRTGGAGIGG VGGGDADGAA TTLDLPAAAT
SITQAEEVAD LLRILRVLHI LATDGVRWIG AYAAAAIAIS PAAFINRRLA AKIMREVANT
QALTSGTFPD WVTDAMSCCP FVLSYDQRVR FFRYTAFGCA LGLRYLTGDA GEGDNQPLAQ
SKVQVSRKHL LASTAHIMHL YATHPTELSV SFVGEVGTGI GPTLEYYART CEQFQRRDLN
LWRDSSSSSS SSSNSSSSTT TTTTGSSGDG GDAVAKAEEA DEEKKEKTKE EEKQTKQEEQ
QQVREGGDTS ATAGDSSEHE FVFSPQGLYP KPVHALGERE GYIFELFGAF VAKAMQDNRP
LDLNLSPAFY GWMVGAPPCT LADVRDVDEG LYKSLVKLQA AADAKKAVDN DTSLDAAARA
EKLAAITVDG CPVEDLCLDF TLPGHPDVSL GQAGADTPVT LENLGEYVAA VVDFTLLRGV
RDAMTSFTAG FAAVFDMRTL TLFTVEEVRT VVCGSRVESW DYDELRACIK AEHGYDSSSP
QVQQLLEVLV ELDVDNKRKF VQFLTGSPNL PVGGFKALKP PFTVVMKDAQ GLSPDQVLPS
VMTCQNYFKM PTYSSKDVLR EKLLMAITEG QGSFDLS
//
