ID F2UBJ2_SALR5 Unreviewed; 440 AA.
AC F2UBJ2;
DT 31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 31-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=tRNA (Uracil-5-)-methyltransferase {ECO:0008006|Google:ProtNLM};
GN ORFNames=PTSG_12338 {ECO:0000313|EMBL:EGD73858.1};
OS Salpingoeca rosetta (strain ATCC 50818 / BSB-021).
OC Eukaryota; Choanoflagellata; Craspedida; Salpingoecidae; Salpingoeca.
OX NCBI_TaxID=946362 {ECO:0000313|Proteomes:UP000007799};
RN [1] {ECO:0000313|Proteomes:UP000007799}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50818 {ECO:0000313|Proteomes:UP000007799};
RA Russ C., Cuomo C., Burger G., Gray M.W., Holland P.W.H., King N.,
RA Lang F.B.F., Roger A.J., Ruiz-Trillo I., Young S.K., Zeng Q., Gargeya S.,
RA Alvarado L., Berlin A., Chapman S.B., Chen Z., Freedman E., Gellesch M.,
RA Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C.,
RA Mehta T., Neiman D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., White J., Yandava C., Haas B., Nusbaum C.,
RA Birren B.;
RT "Annotation of Salpingoeca rosetta.";
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01024}.
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DR EMBL; GL832967; EGD73858.1; -; Genomic_DNA.
DR RefSeq; XP_004993421.1; XM_004993364.1.
DR AlphaFoldDB; F2UBJ2; -.
DR STRING; 946362.F2UBJ2; -.
DR EnsemblProtists; EGD73858; EGD73858; PTSG_12338.
DR GeneID; 16073998; -.
DR KEGG; sre:PTSG_12338; -.
DR eggNOG; KOG2187; Eukaryota.
DR InParanoid; F2UBJ2; -.
DR OMA; QCNTIFV; -.
DR OrthoDB; 52228at2759; -.
DR Proteomes; UP000007799; Unassembled WGS sequence.
DR GO; GO:0030697; F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.40.50.1070; -; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR011869; TrmA_MeTrfase.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR PANTHER; PTHR47790; TRNA/TMRNA (URACIL-C(5))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR47790:SF2; TRNA_TMRNA (URACIL-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000007799};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT REGION 207..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 397
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 299
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 320
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 372
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 440 AA; 47859 MW; 800A968F4A0DCB40 CRC64;
MEGNDDDECS LASKVERLQA ALRAAACGDG GGEGPGCEDD GGVHCTDVAV EASPTTGYRY
RCRFQLLRCC GVDGGEERYA FAVWKHGRPQ AIHSPRDFTI ACARIHTLME RVLEEANCKG
GGDGGDGAGN EVLWQHCQAA HFHCTTTDDA MITFIYDAAI EDEGRWRHEA QRLSRKWGCH
LLARSKGQRL VVGNDYVEEV LTLPPLNDDN TGGTSYPGSS RRQADTTALT TTTTTTTTSN
SQSGTTARSS GAAALRIVLR QPEGAFSNPN PYICLRTMLW LRQRAALVRA DVRHMLELYA
GAGTYTPILG HLFDAVTTVE ISTALVAAAQ HNATANGVDE RVRVVRAPVE AVRHIISSAS
HEGVTFDAVM LDPPRAGADA FTISCLGQYK HIILLSCNPL HTFPRDLRVL CRTHRIASAI
ALDHFPQTPH FEAAMHLIRR
//
