ID F3NG59_9ACTN Unreviewed; 663 AA.
AC F3NG59;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SGM_2123 {ECO:0000313|EMBL:EGG47494.1};
OS Streptomyces griseoaurantiacus M045.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
OX NCBI_TaxID=996637 {ECO:0000313|EMBL:EGG47494.1, ECO:0000313|Proteomes:UP000003022};
RN [1] {ECO:0000313|EMBL:EGG47494.1, ECO:0000313|Proteomes:UP000003022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M045 {ECO:0000313|EMBL:EGG47494.1,
RC ECO:0000313|Proteomes:UP000003022};
RX PubMed=21551298; DOI=10.1128/JB.05053-11;
RA Li F., Jiang P., Zheng H., Wang S., Zhao G., Qin S., Liu Z.;
RT "Draft genome sequence of the marine bacterium Streptomyces
RT griseoaurantiacus M045, which produces novel manumycin-type antibiotics
RT with a pABA core component.";
RL J. Bacteriol. 193:3417-3418(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGG47494.1}.
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DR EMBL; AEYX01000031; EGG47494.1; -; Genomic_DNA.
DR RefSeq; WP_006139778.1; NZ_AEYX01000031.1.
DR AlphaFoldDB; F3NG59; -.
DR STRING; 996637.SGM_2123; -.
DR REBASE; 37164; M2.Sgr45ORF2120P.
DR eggNOG; COG0286; Bacteria.
DR Proteomes; UP000003022; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EGG47494.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000003022};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EGG47494.1}.
FT DOMAIN 9..141
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 153..458
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 663 AA; 73816 MW; 1C78BA5956BABA5E CRC64;
MNSSKHTELA NHAWSVADLL RGDYKQSDYG KVILPFTVLR RLECVLEPTR DAVTETVERF
AGQDINADKF LRKAAGHAFY NTSSLTLKKI AADPGSAAKN LQVYVASFSD NARGVLDRFE
FAQQVKRLDS AGLLYKIIGK FTDLDLRPEV VSNHNMGYIF EELIRRFSEQ SNETAGEHFT
PREVIQLMVR LLVAPDGDAL QLPGVVRTVM DPACGTGGML SATDDLIKEL NPDATVEVYG
QELNPESWAI CRSDLMIKGQ NPENIAFGNS FNDDGHARRT FDYLLANPPF GVEWKKVKED
VEEEHEKLGS AGRFGAGLPR INDGSLLFLQ HMISKMKPVD VNGGGGSRIA IVFNGSPLFT
GAAGSGESEI RRWILENDWL EGIVALPDQL FYNTGISTYF WILTNRKSPD HKGKVVLLDA
RDQWQKMRKS LGDKRKELGK DHIATVVKLY GEALSAAQDA EHPLHAKVKV FDNTAFGYQR
ITVERPLKLR FEVTEETLAA LEASKAIQKL PQASVMLEAF ASLKGSGWSK KTDAWLALKD
AVVQAGSTWP TGAPFNKALR EAIGVRDPEG EVQLVKGKPE ADAELRDYEN VPLGEDVEEY
LEREVHPHVP DAWIDHSKTK IGYEIPFTRH FYVYKPPRPL AEIDAELKLL EAEIQGLLGE
VTE
//
