GenomeNet

Database: UniProt
Entry: F4BEN2
LinkDB: F4BEN2
Original site: F4BEN2 
ID   QUEG_FRACF              Reviewed;         362 AA.
AC   F4BEN2;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   05-DEC-2018, entry version 36.
DE   RecName: Full=Epoxyqueuosine reductase {ECO:0000255|HAMAP-Rule:MF_00916};
DE            EC=1.17.99.6 {ECO:0000255|HAMAP-Rule:MF_00916};
DE   AltName: Full=Queuosine biosynthesis protein QueG {ECO:0000255|HAMAP-Rule:MF_00916};
GN   Name=queG {ECO:0000255|HAMAP-Rule:MF_00916};
GN   OrderedLocusNames=FNFX1_0776;
OS   Francisella cf. novicida (strain Fx1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC   Francisellaceae; Francisella.
OX   NCBI_TaxID=984129;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fx1;
RX   PubMed=21666011; DOI=10.1128/AEM.00337-11;
RA   Siddaramappa S., Challacombe J.F., Petersen J.M., Pillai S., Hogg G.,
RA   Kuske C.R.;
RT   "Common ancestry and novel genetic traits of Francisella novicida-like
RT   isolates from North America and Australia as revealed by comparative
RT   genomic analyses.";
RL   Appl. Environ. Microbiol. 77:5110-5122(2011).
CC   -!- FUNCTION: Catalyzes the conversion of epoxyqueuosine (oQ) to
CC       queuosine (Q), which is a hypermodified base found in the wobble
CC       positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
CC       {ECO:0000255|HAMAP-Rule:MF_00916}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + epoxyqueuosine(34) in tRNA = A + H2O +
CC         queuosine(34) in tRNA; Xref=Rhea:RHEA:32159, Rhea:RHEA-
CC         COMP:10345, Rhea:RHEA-COMP:10346, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:17499, ChEBI:CHEBI:82831,
CC         ChEBI:CHEBI:82834; EC=1.17.99.6; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00916};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00916}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00916}.
CC   -!- SIMILARITY: Belongs to the QueG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00916}.
DR   EMBL; CP002557; AEB27724.1; -; Genomic_DNA.
DR   RefSeq; WP_014549655.1; NC_017450.1.
DR   SMR; F4BEN2; -.
DR   PRIDE; F4BEN2; -.
DR   EnsemblBacteria; AEB27724; AEB27724; FNFX1_0776.
DR   KEGG; fcf:FNFX1_0776; -.
DR   PATRIC; fig|984129.3.peg.772; -.
DR   KO; K18979; -.
DR   OMA; HQFRVFT; -.
DR   BioCyc; FCF984129:G1GSV-777-MONOMER; -.
DR   UniPathway; UPA00392; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0052693; F:epoxyqueuosine reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00916; QueG; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR013542; DUF1730.
DR   InterPro; IPR004453; QueG.
DR   PANTHER; PTHR30002; PTHR30002; 1.
DR   Pfam; PF08331; DUF1730; 1.
DR   TIGRFAMs; TIGR00276; TIGR00276; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Cytoplasm; Iron; Iron-sulfur; Metal-binding; Oxidoreductase;
KW   Queuosine biosynthesis; tRNA processing.
FT   CHAIN         1    362       Epoxyqueuosine reductase.
FT                                /FTId=PRO_0000416071.
FT   DOMAIN      191    220       4Fe-4S ferredoxin-type.
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       200    200       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       203    203       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       206    206       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       210    210       Iron-sulfur 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00916}.
FT   METAL       253    253       Iron-sulfur 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00916}.
FT   METAL       256    256       Iron-sulfur 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00916}.
FT   METAL       260    260       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
SQ   SEQUENCE   362 AA;  41265 MW;  E3E0573851CC2778 CRC64;
     MKLELSLEQW QQVKDFAIRN LNLSSISKAD CDLSEYIPYY NKWLENNYHA DLEYMVKHGS
     KRFIPNELVP GTNSVIVATL NYLNRPVNVK TEVKRLRTTS NIADISIYAH GRDYHKVMKK
     KLQQLGEFID ELTGGHQFRV FTDSAPVLER PLAEKAGLGW QGKSSMLMNK AQGSFFFIGV
     IYSNLDLSKL PDSPKHQDSC GKCQACIKLC PTGAIQPGKM IDSRKCISYL TIENKGSIPL
     ELRDKIGTRI YGCDDCQLVC PFNNYAPITT EKDFQQRDFL VNKPLLELLA WSEKDFDKYT
     QGSAIRRIGY AAWIRNIAIA VGNSPFNQDN IQALELKKLE FSDNQLVLEH LDWAINKQKL
     LS
//
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