ID F4GUH9_PUSST Unreviewed; 1119 AA.
AC F4GUH9;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN OrderedLocusNames=PT7_0089 {ECO:0000313|EMBL:AEC18629.1};
OS Pusillimonas sp. (strain T7-7).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Alcaligenaceae; Pusillimonas.
OX NCBI_TaxID=1007105 {ECO:0000313|EMBL:AEC18629.1, ECO:0000313|Proteomes:UP000008737};
RN [1] {ECO:0000313|EMBL:AEC18629.1, ECO:0000313|Proteomes:UP000008737}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T7-7 {ECO:0000313|EMBL:AEC18629.1,
RC ECO:0000313|Proteomes:UP000008737};
RX PubMed=21622753; DOI=10.1128/JB.05242-11;
RA Cao B., Ma T., Ren Y., Ren Y., Li G., Li P., Guo X., Ding P., Feng L.;
RT "Complete genome sequence of Pusillimonas sp. T7-7, a cold-tolerant diesel
RT oil-degrading bacterium isolated from the Bohai Sea in China.";
RL J. Bacteriol. 193:4021-4022(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T7-7;
RA Cao B., Ma T., Li P., Jiang L., Li Y., Guo X., Feng L.;
RT "Genome Announcement Complete Genome Sequence of Pusillimonas sp. T7-7, a
RT novel species isolated from the Bohai Sea, China.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; CP002663; AEC18629.1; -; Genomic_DNA.
DR RefSeq; WP_013741178.1; NC_015458.1.
DR AlphaFoldDB; F4GUH9; -.
DR STRING; 1007105.PT7_0089; -.
DR KEGG; put:PT7_0089; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_009270_0_0_4; -.
DR OrthoDB; 5905204at2; -.
DR Proteomes; UP000008737; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 5..481
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 493..773
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1119 AA; 122374 MW; 499FF846AED35105 CRC64;
MSIKQPSDSA VRAAAINPTR SFLIQAPAGS GKTELLTDRI LALLATVNRP EEIVAITFTR
KAASEMHARV LAKLKAGQGP QPEEPYKLRG WQLARAVLQR DQQLGWDLLD YPARMSIRTI
DSFCTWLVRA MPWLSSLGGV PSITDNAREH YEAAAQATLS MADENAAVAG LIAHMDVDVR
AAQGLLADML ASRDQWLPLL GQRADIDLLL ENLDSAIEAD LVALARAMPA GWAQALAPSV
STAANILAAS QNTLGLEALQ HWKGEAFSTD ISSLPQWQAL ANMLLTGKNT LRRRVTIKEG
FEAKAVYKDD FLRWLNAASE SEDWVAMLAG IRLAPREGYT DEQHEVLRLL IEALWLAAAQ
LNLRFTEAAE VDFTEISQRA LLALGHADEP TDLLLALDTA IRHILIDEFQ DTSQSQIDLL
ERLTSGWQQG DGRTLFLVGD PMQSIYRFRK ADVGCFLKVK EQGLGDITLT ALELTDNFRS
QANLVDWVNA TCGPVFPKEN HGGLGAIIYT PSVPFNDGLD GISAEYHPVW LRKEDADDTG
DSVLEAESIA IKLAKEALLK YPDSEHPVAI LVRARTHLDG IVHRLAQQNI PCRAVELVSL
RSRQVVIDLA QLARALSHDG DRLAWLSVLR SPLCGLTLNS LHALLGADLT TTVPALLTAW
LARDEAGQPG IEDDEARRLR HAASVLLDTG NTSGAMPFAA WLETRWQALG GADIYHGASD
RNDAERLLRL IEELAPYGGL NPVALEDRLA KLYAAPSSSG RAVEVMTIHK SKGLEFESVI
LAGLHKRPRA DTAPLMRFEM GGGQLLLGPI KHKASDEHDP VSTYLAEREK KRAAYETDRL
LYVALTRARQ QLHLIGVVGL DDKMQIKPPA GSSLLGRLWE HIKTPDVPEA SELGSDAGLT
ATASGGRLLV RMTPDSLPKV PASVAAVGTG TPWQWRPGSG DESVIGTVAH AWLERLGKEG
MAAWPTQRLD QTLPVFRKQL ARAGLAEHAL DASAQVLRDT LVATVESERG AWLLGVARAH
REWSLLDLSG RVSVIDLAIS QEHDWLVVDY KTGVPQEGET PDGFAARMRE RYREQIERYC
AHVSALDGRP ARGALYFPRA DIWIEYATRA VGQNAGLTV
//
