ID F4GW67_PUSST Unreviewed; 131 AA.
AC F4GW67;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=L-ectoine synthase {ECO:0000256|ARBA:ARBA00019707, ECO:0000256|HAMAP-Rule:MF_01255};
DE EC=4.2.1.108 {ECO:0000256|ARBA:ARBA00013192, ECO:0000256|HAMAP-Rule:MF_01255};
DE AltName: Full=N-acetyldiaminobutyrate dehydratase {ECO:0000256|ARBA:ARBA00033271, ECO:0000256|HAMAP-Rule:MF_01255};
GN Name=ectC {ECO:0000256|HAMAP-Rule:MF_01255};
GN OrderedLocusNames=PT7_1541 {ECO:0000313|EMBL:AEC20081.1};
OS Pusillimonas sp. (strain T7-7).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Alcaligenaceae; Pusillimonas.
OX NCBI_TaxID=1007105 {ECO:0000313|EMBL:AEC20081.1, ECO:0000313|Proteomes:UP000008737};
RN [1] {ECO:0000313|EMBL:AEC20081.1, ECO:0000313|Proteomes:UP000008737}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T7-7 {ECO:0000313|EMBL:AEC20081.1,
RC ECO:0000313|Proteomes:UP000008737};
RX PubMed=21622753; DOI=10.1128/JB.05242-11;
RA Cao B., Ma T., Ren Y., Ren Y., Li G., Li P., Guo X., Ding P., Feng L.;
RT "Complete genome sequence of Pusillimonas sp. T7-7, a cold-tolerant diesel
RT oil-degrading bacterium isolated from the Bohai Sea in China.";
RL J. Bacteriol. 193:4021-4022(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=T7-7;
RA Cao B., Ma T., Li P., Jiang L., Li Y., Guo X., Feng L.;
RT "Genome Announcement Complete Genome Sequence of Pusillimonas sp. T7-7, a
RT novel species isolated from the Bohai Sea, China.";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the circularization of gamma-N-acetyl-alpha,gamma-
CC diaminobutyric acid (ADABA) to ectoine (1,4,5,6-tetrahydro-2-methyl-4-
CC pyrimidine carboxylic acid), which is an excellent osmoprotectant.
CC {ECO:0000256|HAMAP-Rule:MF_01255}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S)-4-acetamido-2-aminobutanoate = H2O + L-ectoine;
CC Xref=Rhea:RHEA:17281, ChEBI:CHEBI:15377, ChEBI:CHEBI:58515,
CC ChEBI:CHEBI:58929; EC=4.2.1.108;
CC Evidence={ECO:0000256|ARBA:ARBA00000511, ECO:0000256|HAMAP-
CC Rule:MF_01255};
CC -!- PATHWAY: Amine and polyamine biosynthesis; ectoine biosynthesis; L-
CC ectoine from L-aspartate 4-semialdehyde: step 3/3.
CC {ECO:0000256|ARBA:ARBA00005181, ECO:0000256|HAMAP-Rule:MF_01255}.
CC -!- SIMILARITY: Belongs to the ectoine synthase family.
CC {ECO:0000256|ARBA:ARBA00009637, ECO:0000256|HAMAP-Rule:MF_01255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP002663; AEC20081.1; -; Genomic_DNA.
DR RefSeq; WP_013742617.1; NC_015458.1.
DR AlphaFoldDB; F4GW67; -.
DR STRING; 1007105.PT7_1541; -.
DR KEGG; put:PT7_1541; -.
DR eggNOG; COG1917; Bacteria.
DR HOGENOM; CLU_154525_0_0_4; -.
DR OrthoDB; 9801830at2; -.
DR UniPathway; UPA00067; UER00123.
DR Proteomes; UP000008737; Chromosome.
DR GO; GO:0033990; F:ectoine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0019491; P:ectoine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd06978; cupin_EctC; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR HAMAP; MF_01255; Ectoine_synth; 1.
DR InterPro; IPR010462; Ectoine_synth.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR39289; -; 1.
DR PANTHER; PTHR39289:SF1; L-ECTOINE SYNTHASE; 1.
DR Pfam; PF06339; Ectoine_synth; 1.
DR SUPFAM; SSF51182; RmlC-like cupins; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_01255}.
SQ SEQUENCE 131 AA; 14745 MW; FE873B3BF28C101F CRC64;
MIVRNVKDVI GTEHEVKTET WLSRRVLLQK DGMGFSFHET VIFPGTETHI HYQNHLEAVW
CVEGDGEIET IADGKKYALG PGVVYALDEN DEHWLRGGKE PLRVICVFNP PLTGFEVHDD
KGVYPLVTDA A
//
