GenomeNet

Database: UniProt
Entry: F4KGU4
LinkDB: F4KGU4
Original site: F4KGU4 
ID   DEAHC_ARATH             Reviewed;        1775 AA.
AC   F4KGU4; Q9LX92;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   16-OCT-2019, entry version 67.
DE   RecName: Full=ATP-dependent RNA helicase DEAH12, chloroplastic;
DE            EC=3.6.4.13;
DE   Flags: Precursor;
GN   OrderedLocusNames=At5g10370 {ECO:0000312|Araport:AT5G10370};
GN   ORFNames=F12B17_280 {ECO:0000312|EMBL:CAB89406.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K.,
RA   Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S.,
RA   Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M.,
RA   Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R.,
RA   Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J.,
RA   Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M.,
RA   Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M.,
RA   Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P.,
RA   Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C.,
RA   Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N.,
RA   Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J.,
RA   Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S.,
RA   Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W.,
RA   Ramsperger U., Wedler H., Balke K., Wedler E., Peters S.,
RA   van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R.,
RA   Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W.,
RA   Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H.,
RA   Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA   Xu R., Zhang S., Huang J., Zheng C.;
RT   "Genome-wide comparative in silico analysis of the RNA helicase gene
RT   family in Zea mays and Glycine max: a comparison with Arabidopsis and
RT   Oryza sativa.";
RL   PLoS ONE 8:E78982-E78982(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB89406.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AL353995; CAB89406.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED91533.1; -; Genomic_DNA.
DR   PIR; T50002; T50002.
DR   RefSeq; NP_196599.2; NM_121075.3.
DR   STRING; 3702.AT5G10370.1; -.
DR   iPTMnet; F4KGU4; -.
DR   PaxDb; F4KGU4; -.
DR   EnsemblPlants; AT5G10370.1; AT5G10370.1; AT5G10370.
DR   GeneID; 830901; -.
DR   Gramene; AT5G10370.1; AT5G10370.1; AT5G10370.
DR   KEGG; ath:AT5G10370; -.
DR   Araport; AT5G10370; -.
DR   TAIR; locus:2142459; AT5G10370.
DR   eggNOG; KOG0922; Eukaryota.
DR   eggNOG; KOG1812; Eukaryota.
DR   eggNOG; COG1643; LUCA.
DR   HOGENOM; HOG000084497; -.
DR   InParanoid; F4KGU4; -.
DR   KO; K12818; -.
DR   OMA; YAPPLDM; -.
DR   OrthoDB; 28987at2759; -.
DR   PRO; PR:F4KGU4; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; F4KGU4; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   Gene3D; 1.10.10.2130; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR042035; DEAH_win-hel_dom.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR011709; DUF1605.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR002867; IBR_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR027370; Znf-RING_LisH.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF01485; IBR; 2.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   Pfam; PF13445; zf-RING_UBOX; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00647; IBR; 2.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51873; TRIAD; 1.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chloroplast; Complete proteome; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Plastid; Reference proteome;
KW   Repeat; Transferase; Transit peptide; Ubl conjugation pathway; Zinc;
KW   Zinc-finger.
FT   TRANSIT       1     33       Chloroplast. {ECO:0000255}.
FT   CHAIN        34   1775       ATP-dependent RNA helicase DEAH12,
FT                                chloroplastic.
FT                                /FTId=PRO_0000434938.
FT   DOMAIN      316    480       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      510    676       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     329    336       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   ZN_FING    1564   1612       RING-type 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   ZN_FING    1631   1696       IBR-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   ZN_FING    1722   1750       RING-type 2; atypical.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01221}.
FT   REGION     1560   1767       TRIAD supradomain. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   MOTIF       427    430       DEAH box. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   ACT_SITE   1735   1735       {ECO:0000255|PROSITE-ProRule:PRU01221}.
FT   METAL      1564   1564       Zinc 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1567   1567       Zinc 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1580   1580       Zinc 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1582   1582       Zinc 2; via pros nitrogen.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01221}.
FT   METAL      1585   1585       Zinc 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1588   1588       Zinc 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1607   1607       Zinc 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1612   1612       Zinc 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1652   1652       Zinc 3. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1657   1657       Zinc 3. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1675   1675       Zinc 3. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1678   1678       Zinc 3. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1683   1683       Zinc 4. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1686   1686       Zinc 4. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1691   1691       Zinc 4; via tele nitrogen.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01221}.
FT   METAL      1696   1696       Zinc 4. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1722   1722       Zinc 5. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1725   1725       Zinc 5. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1740   1740       Zinc 5. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
FT   METAL      1742   1742       Zinc 5. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01221}.
SQ   SEQUENCE   1775 AA;  201362 MW;  F3EB71C0F11BDD1C CRC64;
     MRNSFPPSDG GRSATDRRQQ SSHSSSTNRY NSRSAQSSPP LNHRPTWNQQ HSQYPNSNFP
     PNYRRDRNPS SGYSPPVTRA RPNFIVQLLH PAAANSDTKL CFSTKKQEIE SLALLCEIPE
     ESIHVPQFGC IAGSFSFRQW VDARSAVVAL WDYRLQGKHE FVPELIPNVI VPSDMNELKD
     RLRDLFSSHI LSLMENGEGV KKVRLEIEEK SRQVVSFSSK RGLKFEVFEK KKAIEAERDL
     VVNRLEEFNN AMKSILRYLI GQDGYEFDLD DEEEGDVAVF CLEGAYDWRR IHCLIRRECR
     RLEDGLPIYA YRRQILKKIH REQIMVLIGE TGSGKSTQLV QFLADSGVAA SESIVCTQPR
     KIAAMTLADR VREESSGCYE ENTVSCTPTF SSTEEISSKV VYMTDNCLLQ HYMKDRSLSG
     ISCVIIDEAH ERSLNTDLLL ALLKKLLSRR IDLRLVIMSA TADAKQLSQY FFSCGILLVN
     GRNFPVEIVY SPSDTEENSV VGGIASYVGD VVKMAVEIHK TEKEGTILAF LTSQAEVEWA
     CERFITPSAI ALPLHGKLSF EEQFRVFQNH PGRRKVIFAT NIAETSLTIP GVKYVIDSGM
     VKESKYEPRT GMSILKVCRV SQSSARQRAG RAGRTEPGRC YRLYSKNDFD SMNLNQEPEI
     RRVHLGVALL RMLALGVNNI AEFNFVDAPV PEAIAMAVQN LVQLGAVVEK NGVHELTQEG
     HCLVKLGLEP KLGKLILGCF RHRMGKEGIV LAAVMANASS IFCRVGNFDD KMKADRLKVQ
     FCNQNGDLFT LLSVYKEWAS LPRERRNKWC WENSLNAKSM RRCEDTVKEL EICIERELTL
     VSPSYWVWNP NEGTKHDKHL KMVILASLAE NVAMYTGYNQ LGYEVALTGQ QVQLHPSCSL
     LAFGQKPSWV VFGELLSIVD QYLVCVTACD FEALYMLDPP PPFDVSQMDE RRLRIKKVVG
     CSSTVLKRFC GKSNRSLLSI VSRARSLCSD ERIGIQVDVD QNEIRLYAPP LDMEKVSALV
     NDALECEKKW MHNECLEKYL YHGRGQVPIA LFGSGAQIKH LEVDQRFLTV DVLYYGDDVV
     DDRELLTFLE KKIDGSICSI YKFAANKQDC DEKEKWGRIT FLTPESAMKA TEIQKFYFKG
     SVLKLFPSLS TGGGIFKMPY FSSVTAKIRW PRRESSGRGC LKCPSGDIHR ILGDISSLEI
     GTNYVHIQRD QQSNDSILIS GLGDLSEAEV LDVLEFRTQR RDLNFFIFRK KYSVQCPSPT
     ACEEELHKRI FARMSAKNPE PNCVQVQVFE PKEDNYFMRA LIKFDGRLHF EAAKALQELN
     GEVLPGCLPW QKIKCEQLFQ SSIICSASIY NTVKRQLNVL LARFERQKGG ECCLEPTHNG
     AYRVKITAYA TRPVAEMRRE LEELLRGRPI NHPGFTRRVL QHLMSRDGIN LMRKIQQETE
     TYILLDRHNL TVRICGTSEK IAKAEQELIQ ALMDYHESKQ LEIHLRGPEI RPDLMKEVVK
     RFGPELQGIK EKVHGVDLKL NTRYHVIQVH GSKEMRQEVQ KMVNELAREK SALGEKPDEI
     EVECPICLSE VDDGYSLEGC SHLFCKACLL EQFEASMRNF DAFPILCSHI DCGAPIVLAD
     MRALLSQEKL DELFSASLSS FVTSSDGKFR FCSTPDCPSV YRVAGPQESG EPFICGACHS
     EICTRCHLEY HPLITCERYK KFKENPDLSL KDWAKGKNVK ECPICKSTIE KTDGCNHMKC
     RCGKHICWTC LDVFTQEEPC YAHLRTIHGG IGLVE
//
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