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Database: UniProt
Entry: F4KVW0_HALH1
LinkDB: F4KVW0_HALH1
Original site: F4KVW0_HALH1 
ID   F4KVW0_HALH1            Unreviewed;       649 AA.
AC   F4KVW0;
DT   28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT   28-JUN-2011, sequence version 1.
DT   08-MAY-2019, entry version 62.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974};
GN   OrderedLocusNames=Halhy_5712 {ECO:0000313|EMBL:AEE53535.1};
OS   Haliscomenobacter hydrossis (strain ATCC 27775 / DSM 1100 / LMG 10767
OS   / O).
OC   Bacteria; Bacteroidetes; Saprospiria; Saprospirales;
OC   Haliscomenobacteraceae; Haliscomenobacter.
OX   NCBI_TaxID=760192 {ECO:0000313|EMBL:AEE53535.1, ECO:0000313|Proteomes:UP000008461};
RN   [1] {ECO:0000313|EMBL:AEE53535.1, ECO:0000313|Proteomes:UP000008461}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27775 / DSM 1100 / LMG 10767 / O
RC   {ECO:0000313|Proteomes:UP000008461};
RX   PubMed=21886862; DOI=10.4056/sigs.1964579;
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Daligault H., Lapidus A., Zeytun A., Nolan M., Lucas S., Del Rio T.G.,
RA   Tice H., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S.,
RA   Liolios K., Pagani I., Ivanova N., Huntemann M., Mavromatis K.,
RA   Mikhailova N., Pati A., Chen A., Palaniappan K., Land M., Hauser L.,
RA   Brambilla E.M., Rohde M., Verbarg S., Goker M., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.,
RA   Woyke T.;
RT   "Complete genome sequence of Haliscomenobacter hydrossis type strain
RT   (O).";
RL   Stand. Genomic Sci. 4:352-360(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DSM 1100;
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Han J., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA   Peters L., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G.,
RA   Pagani I., Daligault H., Detter J.C., Han C., Land M., Hauser L.,
RA   Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Verbarg S.,
RA   Frueling A., Brambilla E., Klenk H.-P., Eisen J.A.;
RT   "Complete sequence of chromosome of Haliscomenobacter hydrossis DSM
RT   1100.";
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA
CC       replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC       ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00974};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC       Rule:MF_00974};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC       domain that contains the primase activity, and a C-terminal DnaB-
CC       binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
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DR   EMBL; CP002691; AEE53535.1; -; Genomic_DNA.
DR   RefSeq; WP_013768064.1; NC_015510.1.
DR   STRING; 760192.Halhy_5712; -.
DR   EnsemblBacteria; AEE53535; AEE53535; Halhy_5712.
DR   KEGG; hhy:Halhy_5712; -.
DR   eggNOG; ENOG4105C9G; Bacteria.
DR   eggNOG; COG0358; LUCA.
DR   KO; K02316; -.
DR   OMA; PVKQIWK; -.
DR   OrthoDB; 1071997at2; -.
DR   BioCyc; HHYD760192:G1GXE-5769-MONOMER; -.
DR   Proteomes; UP000008461; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR019475; DNA_primase_DnaB-bd.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF10410; DnaB_bind; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000008461};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709339};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008461};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00993442};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN      256    339       Toprim. {ECO:0000259|PROSITE:PS50880}.
FT   ZN_FING      37     61       CHC2-type. {ECO:0000256|HAMAP-Rule:
FT                                MF_00974}.
FT   COILED      597    617       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   649 AA;  74208 MW;  A0CB9049D765704C CRC64;
     MILPKSIQEV NDAAKIEEVV GDFVNLKRRG ANYSGLCPFH NEKSPSFNVN PARNIFKCFG
     CGKAGDPITF VMEHEQLSYP EAVKYLARKY RIELEETESS DQQRQEQQAI DSLFLVNQFA
     QEFFQTQLFD TDKGRSIGLS YFKQRGFREE TLKKFGLGFA QNDMDALLRA GKLRGYNAEL
     LKKLGLVTQY ERDFFRDRVM FSIHNLTGKV IAFAGRIMAK DPKQPKYINS PETEIYYKSK
     VLYGIFFAKK AIRQFDECLL VEGYTDVISL HQAGIENVVA SSGTALTPDQ IRLIKRFTNN
     IKIIYDGDAA GIKAALRGLD LALAEDMNVR LVLLPDGEDP DSYIQKVGSE AFLLYLKEKG
     KDIVQFETEL LLKEAGDDPT RRAGVLGQIA NTISKIKNPL RRDEYVKVTR QVLGVDEGRM
     IFEINKLLRR DLEKHQALEG ITPPEADGSF PTEEPRYQTN PVMPETELSV GDEFQEKDIA
     RILISAGGQW FDQKHNTTVA QYVWINIEDV IDYFDSALYK KVVIEILQRI NQGQDISVQY
     FINHADPEVR QLAATVLTSP YEFSENWEKK HEIFLNQRKP EDNYILDAEL AVGQLKYKKL
     DRLIKQNSAK IKELNNDYDA QSPFIQLNRK LQIMRDELAK KLGIVVVRG
//
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