ID   F4LWC2_TEPAE            Unreviewed;       562 AA.
AC   F4LWC2;
DT   28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT   28-JUN-2011, sequence version 1.
DT   27-MAR-2024, entry version 66.
DE   SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:CDI40785.1};
GN   OrderedLocusNames=TEPIRE1_1702 {ECO:0000313|EMBL:CDI40785.1};
OS   Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1).
OC   Bacteria; Bacillota; Clostridia; Thermosediminibacterales;
OC   Tepidanaerobacteraceae; Tepidanaerobacter.
OX   NCBI_TaxID=1209989 {ECO:0000313|EMBL:CDI40785.1, ECO:0000313|Proteomes:UP000010802};
RN   [1] {ECO:0000313|Proteomes:UP000010802}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Re1 {ECO:0000313|Proteomes:UP000010802};
RX   PubMed=23469343; DOI=10.1128/genomeA.00213-12;
RA   Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.;
RT   "First genome sequence of a syntrophic acetate-oxidizing bacterium,
RT   Tepidanaerobacter acetatoxydans strain Re1.";
RL   Genome Announc. 1:E00213-E00213(2013).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; HF563609; CDI40785.1; -; Genomic_DNA.
DR   RefSeq; WP_013778643.1; NC_019954.2.
DR   AlphaFoldDB; F4LWC2; -.
DR   STRING; 1209989.TepRe1_1580; -.
DR   KEGG; tae:TepiRe1_1702; -.
DR   KEGG; tep:TepRe1_1580; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_021572_1_0_9; -.
DR   OrthoDB; 9801424at2; -.
DR   Proteomes; UP000010802; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   CDD; cd02068; radical_SAM_B12_BD; 1.
DR   Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR   Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR036724; Cobalamin-bd_sf.
DR   InterPro; IPR025288; DUF4080.
DR   InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR   InterPro; IPR034466; Methyltransferase_Class_B.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR   PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF13311; DUF4080; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00022485};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000010802}.
FT   DOMAIN          1..132
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51332"
FT   DOMAIN          167..387
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   562 AA;  65161 MW;  19C5AC881BAA29A4 CRC64;
     MKTLLVGINA KYIHTNLAIR DIAAYCRDED ISVYEATIND NFDDILEDIM SYNADLIGFS
     CYLWNIEDVL YIAENIKKIK SETVIVLGGP EVSYETEELL KRVPHVDYVI LSEGEKRFCE
     LLQAFNGKMP VESIDGLAYR INDNILVNIP KEYVDLNDIP FPYIDEDLEH KLVYYETSRG
     CPFKCAFCIS SLETHVRTAD LEKVKNDLLK FTQMGVKVVK LVDRSFNCNL DRALDLLDII
     RQFPGNTVFH CEINPELVND RFIKALSGIE KQLQFEVGIQ STNPETLKEI SRTPNVKRTI
     EGIKLLKTTG IKLHVDLIAG LPHESFERFG YSFDEVYNLH PAEIQLGFLK LLKGTGLRKY
     AHKYGIVYRT RPPYEILYNN DISYQELCIL KGIALLVDKY YNTGRFRYSM TYLNTKFERP
     FALFMSLYNY CKERNLFRTR HSLKSQYDIL YSFAENVGID TDVFKDIIKF DFMLTSGKAA
     LPECIEPIET REFLNKAKEY AYNKKWLETN LFQAVGLSNH KLSQKLSYGL FRHDVPNNTN
     KKTKGIVFLQ KDGENYYAEF DI
//