ID F4RRA1_MELLP Unreviewed; 333 AA.
AC F4RRA1;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 08-NOV-2023, entry version 47.
DE RecName: Full=Endonuclease {ECO:0000256|RuleBase:RU366055};
DE EC=3.1.30.- {ECO:0000256|RuleBase:RU366055};
GN ORFNames=MELLADRAFT_78192 {ECO:0000313|EMBL:EGG05186.1};
OS Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) (Poplar leaf
OS rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Melampsoraceae; Melampsora.
OX NCBI_TaxID=747676 {ECO:0000313|Proteomes:UP000001072};
RN [1] {ECO:0000313|Proteomes:UP000001072}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=98AG31 / pathotype 3-4-7 {ECO:0000313|Proteomes:UP000001072};
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|RuleBase:RU366055};
CC -!- SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease family.
CC {ECO:0000256|ARBA:ARBA00010052, ECO:0000256|RuleBase:RU366055}.
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DR EMBL; GL883114; EGG05186.1; -; Genomic_DNA.
DR RefSeq; XP_007411551.1; XM_007411489.1.
DR AlphaFoldDB; F4RRA1; -.
DR STRING; 747676.F4RRA1; -.
DR EnsemblFungi; EGG05186; EGG05186; MELLADRAFT_78192.
DR GeneID; 18933077; -.
DR KEGG; mlr:MELLADRAFT_78192; -.
DR VEuPathDB; FungiDB:MELLADRAFT_78192; -.
DR eggNOG; KOG3721; Eukaryota.
DR HOGENOM; CLU_055174_0_2_1; -.
DR InParanoid; F4RRA1; -.
DR OrthoDB; 5487361at2759; -.
DR Proteomes; UP000001072; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:EnsemblFungi.
DR GO; GO:0005736; C:RNA polymerase I complex; IEA:EnsemblFungi.
DR GO; GO:0004529; F:DNA exonuclease activity; IEA:EnsemblFungi.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004521; F:RNA endonuclease activity; IEA:EnsemblFungi.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:EnsemblFungi.
DR GO; GO:0006309; P:apoptotic DNA fragmentation; IEA:EnsemblFungi.
DR GO; GO:0051607; P:defense response to virus; IEA:EnsemblFungi.
DR GO; GO:0006310; P:DNA recombination; IEA:EnsemblFungi.
DR GO; GO:0006401; P:RNA catabolic process; IEA:EnsemblFungi.
DR CDD; cd00091; NUC; 1.
DR Gene3D; 3.40.570.10; Extracellular Endonuclease, subunit A; 1.
DR InterPro; IPR018524; DNA/RNA_endonuclease_AS.
DR InterPro; IPR001604; DNA/RNA_non-sp_Endonuclease.
DR InterPro; IPR044929; DNA/RNA_non-sp_Endonuclease_sf.
DR InterPro; IPR020821; Extracellular_endonuc_su_A.
DR InterPro; IPR044925; His-Me_finger_sf.
DR InterPro; IPR040255; Non-specific_endonuclease.
DR PANTHER; PTHR13966:SF5; ENDONUCLEASE G, MITOCHONDRIAL; 1.
DR PANTHER; PTHR13966; ENDONUCLEASE RELATED; 1.
DR Pfam; PF01223; Endonuclease_NS; 1.
DR SMART; SM00892; Endonuclease_NS; 1.
DR SMART; SM00477; NUC; 1.
DR SUPFAM; SSF54060; His-Me finger endonucleases; 1.
DR PROSITE; PS01070; NUCLEASE_NON_SPEC; 1.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759,
KW ECO:0000256|RuleBase:RU366055}; Hydrolase {ECO:0000256|RuleBase:RU366055};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR640255-2,
KW ECO:0000256|RuleBase:RU366055};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU366055};
KW Reference proteome {ECO:0000313|Proteomes:UP000001072}.
FT DOMAIN 83..300
FT /note="DNA/RNA non-specific endonuclease"
FT /evidence="ECO:0000259|SMART:SM00892"
FT DOMAIN 84..300
FT /note="Extracellular Endonuclease subunit A"
FT /evidence="ECO:0000259|SMART:SM00477"
FT REGION 28..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 102..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 154
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640255-1"
FT BINDING 186
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR640255-2"
SQ SEQUENCE 333 AA; 36915 MW; 182B88CAC7A9DC70 CRC64;
MASTLTQSAL FVAGIVLGAS TAIVYKNKQT DSSSTSSSST TNPNSDSSNQ PIFTRQFHSE
FKSQAPNQIL KFGNPGPIND FFSRQAYAMG YDRRMRNPSW TAEHLTSSNI RPPRSPGGDQ
PDRSHSNFHE DPALPPEFRA KLSDYFRSGY DRGHMVPAAD AKISQEAMNE TFILSNIAPQ
VGEGFNRDYW AHLENFVRQL TTSFSDVYVF TIPLYLPKQD PITQKQIVSY EVIGNPPNVA
VPTHFAKIVY GVNGHQGSLG SFVLPNTKIS NQIPLRSFET PVDSVEKASG LKLFPDQIKS
NSSKLCETVK CEMVIRNFDQ ARKQVTGNKN IGP
//
