ID F5BB17_9VIRU Unreviewed; 1707 AA.
AC F5BB17;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 24-JAN-2024, entry version 69.
DE SubName: Full=Polyprotein {ECO:0000313|EMBL:AED98521.1};
OS Cutthroat trout virus.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Hepelivirales; Hepeviridae; Parahepevirinae; Piscihepevirus;
OC Piscihepevirus heenan.
OX NCBI_TaxID=1016879 {ECO:0000313|EMBL:AED98521.1, ECO:0000313|Proteomes:UP000052110};
RN [1] {ECO:0000313|EMBL:AED98521.1, ECO:0000313|Proteomes:UP000052110}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Heenan88 {ECO:0000313|EMBL:AED98521.1};
RX PubMed=21458509; DOI=10.1016/j.virusres.2011.03.019;
RA Batts W., Yun S., Hedrick R., Winton J.;
RT "A novel member of the family Hepeviridae from cutthroat trout
RT (Oncorhynchus clarkii).";
RL Virus Res. 158:116-123(2011).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; HQ731075; AED98521.1; -; Genomic_RNA.
DR RefSeq; YP_004464917.1; NC_015521.1.
DR SMR; F5BB17; -.
DR GeneID; 10749316; -.
DR KEGG; vg:10749316; -.
DR Proteomes; UP000052110; Genome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23209; ps-ssRNAv_Hepelivirales_RdRp; 1.
DR Gene3D; 3.40.220.10; Leucine Aminopeptidase, subunit E, domain 1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002589; Macro_dom.
DR InterPro; IPR043472; Macro_dom-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF01661; Macro; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SMART; SM00506; A1pp; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52949; Macro domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51154; MACRO; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022807};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Protease {ECO:0000256|ARBA:ARBA00022807};
KW Reference proteome {ECO:0000313|Proteomes:UP000052110};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 58..245
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 716..885
FT /note="Macro"
FT /evidence="ECO:0000259|PROSITE:PS51154"
FT DOMAIN 954..1242
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 1476..1587
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
FT REGION 552..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 679..724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 570..592
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 679..696
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1707 AA; 189629 MW; 2A8534AF4C592765 CRC64;
MDLDCFAHRD HDKVKLAISD CIEKSAGAYV NTSHVITVVL TPEQLEQAKK AFSPQPVTYQ
PRVCLPHALA KHYTTVLEGQ LKNAIRGRPY IEAGPAPHQI NSRPKDTHFC LVRGDARNHV
RFATAPNNGL SRKVKRIYQG IQKEDATACL NGYQGCHQPR ATSIAIHSTY DIPLKEFCRG
MNNHKSDLAH VIMHMPSEIT FPPGVFVNDM TGYRLDTTGE TAKFTYLEDT SNGYDHSKAD
WLEWTTVTAL IVDGFTFHVE VQRRLGSVVY LIIYRSTLPV KITRPLDPLC STSFVFDPYG
PTSGHTAPTS YVRSLHSYAC GLPDETFSYT RLVIYAKGLA FRAKIGAVAL NEGWNPDTTA
FSAVVAGVFM DVAVKRYLRS KGISNCMKEL KKEQNAGFWS LLWQRICIWF GDSNEHRHMD
HNTLKGWVSS GMILDPACMV LQGQANLNVL SLTTTQSGEF EIIGWLASSF EKILDYPNHH
HDYLKDLMPG FTPDRAEHYE NLLHTVLHTK ERQGETDGEL TDRLTEYINV PYEEVHALVT
NRLDLRTRHH IQPKSCLTTP APTPIPTPQS SRSSSPSRTR SSSVSSSSSV STSVISFQME
RESQFEARNQ PQINLLDTPL DANLDLTQAT FLQPSAPPDT NQNQTALQDQ ARAITPTQNP
PDTATLPSTR VLPAATPVDI NTTLQPSPST GAIRKTSPRV PPTVVPPPQN AQVTPIQLPQ
TGQNPRSNIR YHNTSIFDTH CCIAVNPAHP TLKPGGGLSA LFHRQFPDLA AKTAPHTTSF
ERLPPYAVST KVNAVGNISE VVHAIGPDRG RGQTSYNQLL CTYQLAMQVA SGPAAFPLLG
ANIFGWSPED SLRAFMEADT QDMHHLYIYD PSDPGRFRRL IGKDPTDGDQ RPPTRDSMVR
EINGVIEPPR PTLAESIGST LGLAPSWYKP TTHRYDDMAC KAINSNMGME KGKGGDSKVF
AELVASAPLI EPFIFKYHLT EGVPGSGKSH AAKKLNKSTC VVVPTGELKS DWKQSGFKNT
VTPHVAIKYG IKSKFLVVDE VFSIPPHLLA TILYKHRDNN ILLGDSKQIA ACDFDNFRNF
DIIAPKLTPS VYLNLTYRLP QDITRYLQRD YPGIHTASKV PVSISFYPPP SGTPCQVIGY
RQLIKEQPPW KKTANTSHEL QGATRKAVHL HLLTGDVEMI RSSRSHTIVA LSRHTDQIFI
TGDAESIQTL QLTDKMENII INYAPTTLVN DGATLEITST PDDDTHVTAP AADSTAVGEI
LQACTNHLYA PISAVVLDCE KINQGSLTVK CAVPDERIIP KTNFTEIEHC RRANASSTWT
TVRTLIGRYT KLTINPAHVH DTIQAVNKTF WKCLTVDTMN CLPTTAELAE LVRSMKDKGA
DPTLIHDLDF NDISVTKMTF FQKECDKFTM DEPVQHNKPG QGISAWPKTL CFIYGLFFRK
LEKELRQALK PSIIFSNPYT EEQLARAVSK CTTGTTVFYE NDFSAFDSTQ NLVSLGNEFL
ILVQCGMPPA LAEVYFNLRL KWTLDARVAS LRGTSKKHSG EPGTMLFNTI WNMTAVFHTT
SFTRMTLALF KGDDSLVCCD SSILTPTAKE FISQNGLVMK QKTSHLGSFT CSLVTRTSYV
PDVIRFACRL NNKDFTDTKR IEDLQRATDD FIKRATVTTN YGSTCSINGR YYGVPSDTIE
CVVGFLQDFA LTGKIDLQTS YKPILKY
//
