ID F6BDG1_METIK Unreviewed; 372 AA.
AC F6BDG1;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099, ECO:0000256|HAMAP-Rule:MF_00290};
DE EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099, ECO:0000256|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA(m(2,2)G26)dimethyltransferase {ECO:0000256|HAMAP-Rule:MF_00290};
GN Name=trm1 {ECO:0000256|HAMAP-Rule:MF_00290};
GN OrderedLocusNames=Metig_0979 {ECO:0000313|EMBL:AEF96522.1};
OS Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanotorris.
OX NCBI_TaxID=880724 {ECO:0000313|Proteomes:UP000009227};
RN [1] {ECO:0000313|EMBL:AEF96522.1, ECO:0000313|Proteomes:UP000009227}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5666 / JCM 11834 / Kol 5
RC {ECO:0000313|Proteomes:UP000009227};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Mikhailova N., Chertkov O., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ivanova N., Pagani I., Sieprawska-Lupa M.,
RA Whitman W., Woyke T.;
RT "Complete sequence of Methanotorris igneus Kol 5.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Dimethylates a single guanine residue at position 26 of a
CC number of tRNAs using S-adenosyl-L-methionine as donor of the methyl
CC groups. {ECO:0000256|HAMAP-Rule:MF_00290}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43140, Rhea:RHEA-COMP:10359, Rhea:RHEA-COMP:10360,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.216;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00290};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|HAMAP-Rule:MF_00290,
CC ECO:0000256|PROSITE-ProRule:PRU00958}.
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DR EMBL; CP002737; AEF96522.1; -; Genomic_DNA.
DR RefSeq; WP_013799124.1; NC_015562.1.
DR AlphaFoldDB; F6BDG1; -.
DR STRING; 880724.Metig_0979; -.
DR GeneID; 10643825; -.
DR KEGG; mig:Metig_0979; -.
DR HOGENOM; CLU_010862_5_1_2; -.
DR OrthoDB; 372177at2157; -.
DR Proteomes; UP000009227; Chromosome.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0160104; F:tRNA (guanine(26)-N2)-dimethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR HAMAP; MF_00290; tRNA_dimethyltr_TRM1; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR022923; TRM1_arc_bac.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR NCBIfam; TIGR00308; TRM1; 1.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR Pfam; PF02005; TRM; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|HAMAP-
KW Rule:MF_00290}; Reference proteome {ECO:0000313|Proteomes:UP000009227};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_00290};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00290};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00290, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}.
SQ SEQUENCE 372 AA; 41829 MW; 714E225F52A204C1 CRC64;
MIVVEGKTKI TVPDTLTITK KDEVFYNPKM EANRDISVCV IQTFLKNYKR EEFLICDPLG
GSGARGLRYA NELEFKNGIV KVVINDINPK AVKLIKENIK LNNLDNVEVY EGDANVILSK
NFRMFNVVDL DPFGSPSPYL DSGIRATITK NGLLCLTATD TAVLCGRFKK PCVRKYNAFP
INSKDCHEMA IRILIGYAIR VAAKYDIGLK PIFSHATDHY VRTYLLTQRG AKKADEALEK
LGYVKDIGGD KVIKSLYEGY EKGFGGPLYL GEIYDKEIVN EVYKIAVERN YSEKAKNILK
VLCDECDINQ LGCYDIHEIC SNIKKSAPPM TTIIEKLKER GFKVSRVHYN PKGIKSDAKL
SDIIEVILEA TD
//
