ID F6DL54_DESRL Unreviewed; 1111 AA.
AC F6DL54;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=Desru_1000 {ECO:0000313|EMBL:AEG59275.1};
OS Desulforamulus ruminis (strain ATCC 23193 / DSM 2154 / NCIMB 8452 / DL)
OS (Desulfotomaculum ruminis).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae;
OC Desulforamulus.
OX NCBI_TaxID=696281 {ECO:0000313|EMBL:AEG59275.1, ECO:0000313|Proteomes:UP000009234};
RN [1] {ECO:0000313|Proteomes:UP000009234}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23193 / DSM 2154 / NCIB 8452 / DL
RC {ECO:0000313|Proteomes:UP000009234};
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N.,
RA Ivanova N., Mikhailova N., Pagani I., Stams A.J.M., Plugge C.M., Muyzer G.,
RA Kuever J., Parshina S.N., Ivanova A.E., Nazina T.N., Brambilla E.,
RA Spring S., Klenk H.-P., Woyke T.;
RT "Complete sequence of Desulfotomaculum ruminis DSM 2154.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AEG59275.1, ECO:0000313|Proteomes:UP000009234}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23193 / DSM 2154 / NCIB 8452 / DL
RC {ECO:0000313|Proteomes:UP000009234};
RX PubMed=23408247; DOI=10.4056/sigs.3226659;
RA Spring S., Visser M., Lu M., Copeland A., Lapidus A., Lucas S., Cheng J.F.,
RA Han C., Tapia R., Goodwin L.A., Pitluck S., Ivanova N., Land M., Hauser L.,
RA Larimer F., Rohde M., Goker M., Detter J.C., Kyrpides N.C., Woyke T.,
RA Schaap P.J., Plugge C.M., Muyzer G., Kuever J., Pereira I.A.,
RA Parshina S.N., Bernier-Latmani R., Stams A.J., Klenk H.P.;
RT "Complete genome sequence of the sulfate-reducing firmicute
RT Desulfotomaculum ruminis type strain (DL(T)).";
RL Stand. Genomic Sci. 7:304-319(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP002780; AEG59275.1; -; Genomic_DNA.
DR RefSeq; WP_013841046.1; NC_015589.1.
DR AlphaFoldDB; F6DL54; -.
DR STRING; 696281.Desru_1000; -.
DR KEGG; dru:Desru_1000; -.
DR eggNOG; COG0210; Bacteria.
DR eggNOG; COG1379; Bacteria.
DR HOGENOM; CLU_005571_0_0_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000009234; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd19067; PfuEndoQ-like; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000009234}.
FT DOMAIN 473..743
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 744..1024
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 434..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 494..501
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1111 AA; 123990 MW; 2D8DAFE4E6785EF3 CRC64;
MFIADFHIHS KYSRATSRDC VPEILEMWAR RKGINVIGTG DFTHPAWREE LKEKLIPSGE
GLYVLKNNFR QEDPVAGADF QPQFIVSGEI SSIYKKNGRV RKVHNLILLP GLDYAESISR
RLEAIGNLHS DGRPILGLDS RDLLEIVLEQ CPEAIFIPAH IWTPHFSLYG AYSGFDHIQE
CFEDLTPYIY ALETGLSSDP PMNWRLSALD QFTLISNSDA HSPANLAREA NLFAAEPSYP
GILQALKNRH SQEFYGTLEF FPEEGKYHYD GHRACKVCWK PADTIAAAGL CPVCGGKITV
GVLHRLEALA DRKEGYIPPA AKHFESLVPL HEVIASSMGL SAASVKVKGK YQDLIRNLGP
ELFILREATL QDIELAAGPY IAEGVRRLRC GKIDIQPGFD GEYGKIKVMD KSEITLLSGQ
LCFLSSSTTE NQPSALKEKK AAQALPPAPT SPDACLPEGG EAAIKTDSPD RPYGLNPEQW
EAVSSPHPAI AVMAGPGTGK TKTLVGRIAH MVENCGVDPS RITAVTFTNQ AAKEMRLRLE
IHFGDKRTAR AMTIGTFHSL CLQILSKGKD GKNIVLIDEQ NALSMVGDIL KDLQLKISPR
EAMKQISLLK NDASATGDKK KSDVPAEVHE LYRSQLQRYG VLDYDDLLLE VLKLFEGSPE
ERKNRRLEKP FSYLLVDEFQ DINEVQYRLL KAWGRKSENI FIIGDPDQSI YGFRGSDFRY
FEKFKEDFPG LCQVRLVQNY RSTPEIIHCA TSVISREPFL GPERCLEAKR AKGAPVRLIE
AQNEFSEALF VAKEINRMVG GIDMLDTLKG SAANKKRPVP EHPRAFSDIA ILYRTNRQAR
ILEQCLLQEG IQYVVAGRDE FLGEQPVREA IAFFSFLLNP GDMLSLRLFL QAESTYTADL
RQKVLEEYAA LEKNVSSLVG ILERIAPPPN IPDKHGNLIE RLRKYEPMVH KEKPGKLMAA
WINDNALSDL KSMELLLQTA VLYDKMPSFM QNLVLGQESD VVRNGGKAYS SDAVSLMTLH
GAKGLEFPVV FLCGVNDGLI PLKNSKQDLD PGEERRLFYV GLTRARDELL LLTSRTPSPF
IDDLPEENLV TEKAGTPKQG PQFKQVSLFN L
//
