ID F6EHW7_HOYSD Unreviewed; 584 AA.
AC F6EHW7;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN OrderedLocusNames=AS9A_0458 {ECO:0000313|EMBL:AEF38915.1};
OS Hoyosella subflava (strain DSM 45089 / JCM 17490 / NBRC 109087 / DQS3-9A1)
OS (Amycolicicoccus subflavus).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Hoyosellaceae;
OC Hoyosella.
OX NCBI_TaxID=443218 {ECO:0000313|EMBL:AEF38915.1, ECO:0000313|Proteomes:UP000009235};
RN [1] {ECO:0000313|EMBL:AEF38915.1, ECO:0000313|Proteomes:UP000009235}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45089 / DQS3-9A1 {ECO:0000313|Proteomes:UP000009235};
RX PubMed=21725023; DOI=10.1128/JB.05388-11;
RA Cai M., Chen W.M., Nie Y., Chi C.Q., Wang Y.N., Tang Y.Q., Li G.Y.,
RA Wu X.L.;
RT "Complete genome sequence of Amycolicicoccus subflavus DQS3-9A1T, an
RT actinomycete isolated from crude oil-polluted soil.";
RL J. Bacteriol. 193:4538-4539(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP002786; AEF38915.1; -; Genomic_DNA.
DR AlphaFoldDB; F6EHW7; -.
DR STRING; 443218.AS9A_0458; -.
DR KEGG; asd:AS9A_0458; -.
DR eggNOG; COG1020; Bacteria.
DR HOGENOM; CLU_024186_4_3_11; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000009235; Chromosome.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:AEF38915.1};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000009235};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AEF38915.1}.
FT DOMAIN 128..382
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 423..573
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 584 AA; 63704 MW; FC08D2E25B098A85 CRC64;
MSVPVVFVSV SPRSPSGSSN HITPANESAL RHSGVAQREI LLAALHIPNS QLGCNFNKKW
PIFDHETFLT SNATVRQLLE CSTIRVVRCI PVRDRNISAA EMPDVHRMNG SAPVQQLDEI
SSLMFLRERA EYRPHLISVY FLEGGMTESA SSIRAHILSC LADNPAFAVR LAKVPMRFGH
PFWAPLAAID ADTHIRFRAV SGRGDWQALR SDLVELLEAP MDPTRPLWEV HVLTGFEDES
AAPGGRTIVA VKVHHGVMDG LGLARFAGHL FTEAAMHPQK VNGTRANSAP PIDARVRLRT
AARNAAAQFL GFPRELTSLR NLKRRYDASV ARGERPGIRQ TAPGTVFNGP TQGRRVFNAA
WFPLKEVQAL RTLAPGATLN DVALTVIAGA IREYLSSKGT LPDEALLCGV PMSLRNSEEP
VLGNTLTGLC VDLHTNVADP IDRLHRISGS SYWEKQRVRE FGAVIGSGAL ALKLPPFILD
LILKGEEAAA VKGERVTGAN TVVTNIPRSR HVRYFATSRV LRTCGLISLN PGIGLKHEIS
SLGDLVSISV LANSDQLDAP DFYIQCLSNE FGRLMSARAT EPVR
//
