ID F6H6T0_VITVI Unreviewed; 797 AA.
AC F6H6T0;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 24-JAN-2024, entry version 54.
DE RecName: Full=digalactosyldiacylglycerol synthase {ECO:0000256|ARBA:ARBA00024055};
DE EC=2.4.1.241 {ECO:0000256|ARBA:ARBA00024055};
GN OrderedLocusNames=VIT_08s0105g00260 {ECO:0000313|EMBL:CCB47916.1};
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760 {ECO:0000313|EMBL:CCB47916.1, ECO:0000313|Proteomes:UP000009183};
RN [1] {ECO:0000313|Proteomes:UP000009183}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir / PN40024 {ECO:0000313|Proteomes:UP000009183};
RX PubMed=17721507; DOI=10.1038/nature06148;
RG The French-Italian Public Consortium for Grapevine Genome Characterization.;
RA Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA Wincker P.;
RT "The grapevine genome sequence suggests ancestral hexaploidization in major
RT angiosperm phyla.";
RL Nature 449:463-467(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-
CC D-galactose = 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-
CC galactosyl]-sn-glycerol + H(+) + UDP; Xref=Rhea:RHEA:10520,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17615, ChEBI:CHEBI:28396,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.241;
CC Evidence={ECO:0000256|ARBA:ARBA00023914};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC Plastid, chloroplast outer membrane {ECO:0000256|ARBA:ARBA00024013}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000256|ARBA:ARBA00009481}.
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DR EMBL; FN595245; CCB47916.1; -; Genomic_DNA.
DR RefSeq; XP_002264659.1; XM_002264623.4.
DR AlphaFoldDB; F6H6T0; -.
DR STRING; 29760.F6H6T0; -.
DR PaxDb; 29760-VIT_08s0105g00260-t01; -.
DR EnsemblPlants; Vitvi08g00637_t001; Vitvi08g00637_P001; Vitvi08g00637.
DR GeneID; 100259167; -.
DR Gramene; Vitvi08g00637_t001; Vitvi08g00637_P001; Vitvi08g00637.
DR KEGG; vvi:100259167; -.
DR eggNOG; ENOG502QQ73; Eukaryota.
DR HOGENOM; CLU_011647_0_1_1; -.
DR InParanoid; F6H6T0; -.
DR OrthoDB; 276624at2759; -.
DR Proteomes; UP000009183; Chromosome 8.
DR ExpressionAtlas; F6H6T0; baseline and differential.
DR GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0046481; F:digalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0035250; F:UDP-galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0019375; P:galactolipid biosynthetic process; IBA:GO_Central.
DR CDD; cd01635; Glycosyltransferase_GTB-type; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR044525; DGDG1/2.
DR InterPro; IPR001296; Glyco_trans_1.
DR PANTHER; PTHR46132:SF6; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 1, CHLOROPLASTIC; 1.
DR PANTHER; PTHR46132; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00022805};
KW Plastid {ECO:0000256|ARBA:ARBA00022805};
KW Plastid outer membrane {ECO:0000256|ARBA:ARBA00022805};
KW Reference proteome {ECO:0000313|Proteomes:UP000009183};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 552..687
FT /note="Glycosyl transferase family 1"
FT /evidence="ECO:0000259|Pfam:PF00534"
SQ SEQUENCE 797 AA; 90307 MW; C42DC5B050F6ED39 CRC64;
MINIKTETRA TAAPEPVSSA NAFSFLSRGW REVRDSADAD LKLMKNRADS FKNLATSFDR
EIENFINSAS AFSVPAIKSS PPAEIDFVKR LQPKISEIRR AYSSPDFSRK VLEKWSPRTR
IRIDLSAIKN AIVADAEERD GGLGFRGWER VRRGRGLRLK EFWGEWKEES EEGQKEWEPI
RALKTRLQRR SSSSDIFEGF KNSEFVEKVK SSLKAICREP QESKDVPPLD VPELLAYLVR
QSGPFLDQLG FKTDICDKIV ESLCSKRKNQ LLLRSLSAGE SSFLESDNTN DELDLRIASV
LQSTGHCYEG GFWADSAKHN LSDGKRHVAI VTTASLPWMT GTAVNPLFRA AYLASYAKQN
VTLLVPWLCK KDQELVYPNS LTFSSPEEQE VYIRNWLEER VGFKADFKIS FYPGKFSKSR
RSIIPAGDTS QFIPSRDADI AILEEPEHLN WYHHGKRWTD KFNHVVGVVH TNYLEYIKRE
KNGALQAFFV KHINNWVARA YCHKVLRLSA ATQDLPKSVI CNVHGVNPKF LKIGEKLAEE
RELGQRAFSK GAYFLGKMVW AKGYRELIDL LSRHKNDLDG FNLDVFGNGE DAHEVQTAAK
RLHLNLNFMK GRDHADDSLH GYKVFINPSV SDVLCTATAE ALAMGKFVIC ADHPSNEFFS
SFPNCLTYKT SDDFVAKVKE ALANEPQPLT PEQRYNLSWE AATQRFMEYS DLDRVLNNKD
DAQLSKSCGK LITRSVSMPT LSGMVDGGLA FAHYCLTGNE LLRLCTGAIP GTRDYDKQHC
RDLHLLPPQV ENPIYGW
//
