ID F6I071_VITVI Unreviewed; 329 AA.
AC F6I071;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=CN hydrolase domain-containing protein {ECO:0000259|PROSITE:PS50263};
GN OrderedLocusNames=VIT_02s0033g00660 {ECO:0000313|EMBL:CCB60337.1};
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760 {ECO:0000313|EMBL:CCB60337.1, ECO:0000313|Proteomes:UP000009183};
RN [1] {ECO:0000313|Proteomes:UP000009183}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir / PN40024 {ECO:0000313|Proteomes:UP000009183};
RX PubMed=17721507; DOI=10.1038/nature06148;
RG The French-Italian Public Consortium for Grapevine Genome Characterization.;
RA Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA Wincker P.;
RT "The grapevine genome sequence suggests ancestral hexaploidization in major
RT angiosperm phyla.";
RL Nature 449:463-467(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-cyano-L-alanine + 2 H2O = L-aspartate + NH4(+);
CC Xref=Rhea:RHEA:11188, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29991, ChEBI:CHEBI:77860; EC=3.5.5.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000322};
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC Nitrilase family. {ECO:0000256|ARBA:ARBA00008129}.
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DR EMBL; FN596505; CCB60337.1; -; Genomic_DNA.
DR AlphaFoldDB; F6I071; -.
DR STRING; 29760.F6I071; -.
DR PaxDb; 29760-VIT_02s0033g00660-t01; -.
DR EnsemblPlants; Vitvi02g01607_t001; Vitvi02g01607_P001; Vitvi02g01607.
DR Gramene; Vitvi02g01607_t001; Vitvi02g01607_P001; Vitvi02g01607.
DR eggNOG; KOG0805; Eukaryota.
DR HOGENOM; CLU_030130_6_1_1; -.
DR InParanoid; F6I071; -.
DR Proteomes; UP000009183; Chromosome 2.
DR GO; GO:0047427; F:cyanoalanine nitrilase activity; IEA:UniProtKB-EC.
DR GO; GO:0000257; F:nitrilase activity; IBA:GO_Central.
DR GO; GO:0018822; F:nitrile hydratase activity; IBA:GO_Central.
DR GO; GO:0051410; P:detoxification of nitrogen compound; IBA:GO_Central.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR CDD; cd07564; nitrilases_CHs; 1.
DR Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR InterPro; IPR044149; Nitrilases_CHs.
DR PANTHER; PTHR46044:SF15; BIFUNCTIONAL NITRILASE_NITRILE HYDRATASE NIT4B-RELATED; 1.
DR PANTHER; PTHR46044; NITRILASE; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
DR PROSITE; PS00920; NITRIL_CHT_1; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000009183}.
FT DOMAIN 5..273
FT /note="CN hydrolase"
FT /evidence="ECO:0000259|PROSITE:PS50263"
FT ACT_SITE 45
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10139"
SQ SEQUENCE 329 AA; 36599 MW; D55EBA7243F9113D CRC64;
MSSTVRATVV QASSIFYDTP ATLDKAERFL KEAAALGSQL VVFPEAFIGG YPRGYNFADQ
SPRGKESFRK YHASAINVPG PEADRLASMA AKYKVYLVTG VVERDGYTLY STVLFFDPEG
NYLGKHRKLM PTYWERLFWG FGDCSTTPVY DTPYGKLGSV ICWENRMPLF RTAMYGKGIE
IYCAPTADSG DTWVATMRHV AIEGGCYVLS PIQFCRRKDY PPPPEYLYSP TEEDVTPDSI
VWAGGSVIIS PHGEILAGPN YEGEGLFTAD LDVRGEIPKA KFQFDVVGHY SRADVLSLTV
NNRPLLPVTF TSSPSKIKDN EEMDECKDI
//
