ID F6SBJ2_CIOIN Unreviewed; 299 AA.
AC F6SBJ2;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 2.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=GCS light chain {ECO:0000256|ARBA:ARBA00031732};
DE AltName: Full=Gamma-ECS regulatory subunit {ECO:0000256|ARBA:ARBA00030406};
DE AltName: Full=Gamma-glutamylcysteine synthetase regulatory subunit {ECO:0000256|ARBA:ARBA00032926};
DE AltName: Full=Glutamate--cysteine ligase modifier subunit {ECO:0000256|ARBA:ARBA00031154};
OS Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis).
OC Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC Cionidae; Ciona.
OX NCBI_TaxID=7719 {ECO:0000313|Ensembl:ENSCINP00000026337.2, ECO:0000313|Proteomes:UP000008144};
RN [1] {ECO:0000313|Proteomes:UP000008144}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12481130; DOI=10.1126/science.1080049;
RA Dehal P., Satou Y., Campbell R.K., Chapman J., Degnan B., De Tomaso A.,
RA Davidson B., Di Gregorio A., Gelpke M., Goodstein D.M., Harafuji N.,
RA Hastings K.E., Ho I., Hotta K., Huang W., Kawashima T., Lemaire P.,
RA Martinez D., Meinertzhagen I.A., Necula S., Nonaka M., Putnam N., Rash S.,
RA Saiga H., Satake M., Terry A., Yamada L., Wang H.G., Awazu S., Azumi K.,
RA Boore J., Branno M., Chin-Bow S., DeSantis R., Doyle S., Francino P.,
RA Keys D.N., Haga S., Hayashi H., Hino K., Imai K.S., Inaba K., Kano S.,
RA Kobayashi K., Kobayashi M., Lee B.I., Makabe K.W., Manohar C., Matassi G.,
RA Medina M., Mochizuki Y., Mount S., Morishita T., Miura S., Nakayama A.,
RA Nishizaka S., Nomoto H., Ohta F., Oishi K., Rigoutsos I., Sano M.,
RA Sasaki A., Sasakura Y., Shoguchi E., Shin-i T., Spagnuolo A., Stainier D.,
RA Suzuki M.M., Tassy O., Takatori N., Tokuoka M., Yagi K., Yoshizaki F.,
RA Wada S., Zhang C., Hyatt P.D., Larimer F., Detter C., Doggett N.,
RA Glavina T., Hawkins T., Richardson P., Lucas S., Kohara Y., Levine M.,
RA Satoh N., Rokhsar D.S.;
RT "The draft genome of Ciona intestinalis: insights into chordate and
RT vertebrate origins.";
RL Science 298:2157-2167(2002).
RN [2] {ECO:0000313|Ensembl:ENSCINP00000026337.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2. {ECO:0000256|ARBA:ARBA00005006}.
CC -!- SUBUNIT: Heterodimer of a catalytic heavy chain and a regulatory light
CC chain. {ECO:0000256|ARBA:ARBA00011532}.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family.
CC Glutamate--cysteine ligase light chain subfamily.
CC {ECO:0000256|ARBA:ARBA00008612}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EAAA01001108; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; F6SBJ2; -.
DR STRING; 7719.ENSCINP00000026337; -.
DR Ensembl; ENSCINT00000026583.2; ENSCINP00000026337.2; ENSCING00000014616.2.
DR GeneTree; ENSGT00510000047658; -.
DR HOGENOM; CLU_055657_1_0_1; -.
DR InParanoid; F6SBJ2; -.
DR OMA; IWIRSRS; -.
DR TreeFam; TF105986; -.
DR UniPathway; UPA00142; UER00209.
DR Proteomes; UP000008144; Chromosome 13.
DR GO; GO:0017109; C:glutamate-cysteine ligase complex; IBA:GO_Central.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0035226; F:glutamate-cysteine ligase catalytic subunit binding; IEA:InterPro.
DR GO; GO:0006750; P:glutathione biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR032963; Gclm.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR13295; GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT; 1.
DR PANTHER; PTHR13295:SF4; GLUTAMATE--CYSTEINE LIGASE REGULATORY SUBUNIT; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW Glutathione biosynthesis {ECO:0000256|ARBA:ARBA00022684};
KW Reference proteome {ECO:0000313|Proteomes:UP000008144}.
FT DOMAIN 88..228
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
SQ SEQUENCE 299 AA; 33963 MW; 0982D41F7966EF0E CRC64;
MDTDRNEVAR IMVGHASHIR LHTGNVINWN LLKKHKFRPA MTSSDELMDA MHTAMQTWIR
SMNIESPNLP EPRNILDVSN TELVAPIDSE QRDQMKLSVK LFLTRWDPEL IRQAVDHVTR
ELGVQRIDSV IVSLPISPPT SLLEPNNNST SVIHDLEKVK PIWKEMQSTV DSGHVTNIGV
CDFDQESLKE LNDWARIKPS TNQINLTNCC AVPCDLIQYS REKSIELLTH NDPAEILSGR
KFQSFMSEAT DDINSTSWSP AYVARYSVLV RYRGIVHSKG YLLSAERSSQ DGAEDYGFF
//
