ID F6SRM7_HORSE Unreviewed; 617 AA.
AC F6SRM7;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 2.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=Transporter {ECO:0000256|RuleBase:RU003732};
GN Name=SLC6A3 {ECO:0000313|Ensembl:ENSECAP00000022222.2,
GN ECO:0000313|VGNC:VGNC:23260};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000022222.2, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000022222.2, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000022222.2,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000022222.2}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000022222.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-noradrenaline(out) + chloride(out) + Na(+)(out) = (R)-
CC noradrenaline(in) + chloride(in) + Na(+)(in); Xref=Rhea:RHEA:70923,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:29101, ChEBI:CHEBI:72587;
CC Evidence={ECO:0000256|ARBA:ARBA00034288};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + dopamine(out) + 2 Na(+)(out) = chloride(in) +
CC dopamine(in) + 2 Na(+)(in); Xref=Rhea:RHEA:70931, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:59905;
CC Evidence={ECO:0000256|ARBA:ARBA00034267};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + dopamine(out) + Na(+)(out) = chloride(in) +
CC dopamine(in) + Na(+)(in); Xref=Rhea:RHEA:70919, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:59905;
CC Evidence={ECO:0000256|ARBA:ARBA00034275};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Cell
CC projection, axon {ECO:0000256|ARBA:ARBA00004489}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A3 subfamily. {ECO:0000256|ARBA:ARBA00007836}.
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DR AlphaFoldDB; F6SRM7; -.
DR SMR; F6SRM7; -.
DR STRING; 9796.ENSECAP00000022222; -.
DR PaxDb; 9796-ENSECAP00000022222; -.
DR Ensembl; ENSECAT00000026618.4; ENSECAP00000022222.2; ENSECAG00000024615.4.
DR VGNC; VGNC:23260; SLC6A3.
DR GeneTree; ENSGT00940000161224; -.
DR HOGENOM; CLU_006855_9_0_1; -.
DR InParanoid; F6SRM7; -.
DR OMA; KRWKYAG; -.
DR OrthoDB; 3084493at2759; -.
DR TreeFam; TF343812; -.
DR Proteomes; UP000002281; Chromosome 21.
DR Bgee; ENSECAG00000024615; Expressed in adult mammalian kidney and 3 other cell types or tissues.
DR GO; GO:0043679; C:axon terminus; IEA:Ensembl.
DR GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR GO; GO:0016600; C:flotillin complex; IEA:Ensembl.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0032809; C:neuronal cell body membrane; IBA:GO_Central.
DR GO; GO:0042734; C:presynaptic membrane; IBA:GO_Central.
DR GO; GO:0035240; F:dopamine binding; IEA:Ensembl.
DR GO; GO:0005330; F:dopamine:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0005334; F:norepinephrine:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0021984; P:adenohypophysis development; IEA:Ensembl.
DR GO; GO:0050890; P:cognition; IEA:Ensembl.
DR GO; GO:0042416; P:dopamine biosynthetic process; IEA:Ensembl.
DR GO; GO:0042420; P:dopamine catabolic process; IEA:Ensembl.
DR GO; GO:0051583; P:dopamine uptake involved in synaptic transmission; IBA:GO_Central.
DR GO; GO:1990384; P:hyaloid vascular plexus regression; IEA:Ensembl.
DR GO; GO:0007595; P:lactation; IEA:Ensembl.
DR GO; GO:0007626; P:locomotory behavior; IEA:Ensembl.
DR GO; GO:0015874; P:norepinephrine transport; IBA:GO_Central.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IEA:Ensembl.
DR GO; GO:0060134; P:prepulse inhibition; IEA:Ensembl.
DR GO; GO:0042053; P:regulation of dopamine metabolic process; IEA:Ensembl.
DR GO; GO:0042220; P:response to cocaine; IEA:Ensembl.
DR GO; GO:0007608; P:sensory perception of smell; IEA:Ensembl.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002436; Na/ntran_symport_dopamine.
DR InterPro; IPR037272; SNS_sf.
DR NCBIfam; NF037979; Na_transp; 1.
DR PANTHER; PTHR11616:SF38; SODIUM-DEPENDENT DOPAMINE TRANSPORTER; 1.
DR PANTHER; PTHR11616; SODIUM/CHLORIDE DEPENDENT TRANSPORTER; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01202; DOPTRANSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SNF-like; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Symport {ECO:0000256|RuleBase:RU003732};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU003732};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU003732}.
FT TRANSMEM 69..87
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 99..120
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 141..168
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 235..252
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 264..287
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 307..329
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 389..415
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 444..469
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 475..498
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 519..541
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 553..575
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 617 AA; 68854 MW; CE7DAEA0622E4C72 CRC64;
MNRSKCSVGL MSSAVAPATE PNAVGPKAVE LVLIKEQNGV QLTNSTLINP PQTPVENQHR
ETWSKKVDFL LSVVGFAVDL ANVWRFPYLC YKNGGGAFLV PYLLFMVIAG MPLFYMELAL
GQFNREGAAG VWKICPVLKG VGFAVILVSL YVGFFYNVII AWALHYFFSS FTTELPWTHC
NNTWNSPNCS DTRSGNSSSS LNDTFRTTPA AEYFERGMLH LHESRGIDDL GPPRWQLTFC
LVLVIILLYF SLWKGVKTSG KVVWITATMP YVVLFALLLR GITLPGAMDG IRAYLSVDFH
RLCEASVWID AAIQICFSLG IGFGVLIAFS SYNKFTNNCY RDAIITTSVN SLTSFSSGFV
VFSFLGYMAQ KHSVPIGDVA KDGPGLIFII YPEALATLPL SSAWAVVFFV MLLTLGIDSA
MGGMESVITG LIDEFQLLHR HRELFTLFIV LATFLLSLFC VTNGGIYVFT LLDHFAAGTS
ILFGVLIEAI GVAWFYGVRQ FSDDIKQMTG KRPSLYWRLC WKFVSPCFLL FVVVVSIVTF
RPPHYGAYVF PEWANVLGWA IAVSSMSMVP IYAAYKFCSL PGSWREKLAY AITPEKEREL
VERGEVRQFT FRHWLVV
//
