ID F6TE92_HORSE Unreviewed; 1541 AA.
AC F6TE92;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 3.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN Name=AGL {ECO:0000313|Ensembl:ENSECAP00000009911.3,
GN ECO:0000313|VGNC:VGNC:15161};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000009911.3, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000009911.3, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000009911.3,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000009911.3}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000009911.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
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DR Ensembl; ENSECAT00000012545.3; ENSECAP00000009911.3; ENSECAG00000010571.4.
DR VGNC; VGNC:15161; AGL.
DR GeneTree; ENSGT00390000012596; -.
DR HOGENOM; CLU_001517_2_0_1; -.
DR InParanoid; F6TE92; -.
DR OMA; YEEGHVH; -.
DR OrthoDB; 1427975at2759; -.
DR TreeFam; TF300697; -.
DR Proteomes; UP000002281; Chromosome 5.
DR Bgee; ENSECAG00000010571; Expressed in gluteus medius and 23 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0016234; C:inclusion body; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IBA:GO_Central.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:Ensembl.
DR GO; GO:0030247; F:polysaccharide binding; IEA:Ensembl.
DR GO; GO:0031593; F:polyubiquitin modification-dependent protein binding; IEA:Ensembl.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IBA:GO_Central.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 1: Evidence at protein level;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Proteomics identification {ECO:0007829|PeptideAtlas:F6TE92};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 31..115
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 121..552
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 698..983
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1082..1536
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1541 AA; 175616 MW; A4E694E8C060EA41 CRC64;
MGHSKQIRIL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY PFPGETFNRE
KFHSLQWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE KSGGGYIVVD PILRVGADNH
VLPLDCVTLQ TFLTKCLGPF DEWESRLRVA KESGYNMIHF TPLQTLGLSR SSYSLADQLE
LNPDFSRPNK KYTWHDVGQL VEKLKKEWDI LCITDVVYNH TAANSKWIHE HPESAYNLVN
SPHLKPAWVL DRALWHLSCD VAEGKYREKG VPALIENDHQ MNCIRKIIWE DIYPKIHLWE
FFQVDVHKAV EQFRGLLTQE NRKIISQPDP KQHLKIIQDP EYRRLGCTVD MNIALATFIP
HDNGPAAIDE CCNWFRKRIE ELNAEKHQLV NYHQEQAVNC LLGNVFYERL AGHGPKLGPV
TRKHPLVTRY FTFPFEEMTP STEESMIHLP NKACFLMAHN GWVMGDDPLR NFAEPGSDVY
LRRELICWGD SVKLRYGNKP EDCPYLWAHM KKYTEITATH FQGVRLDNCH STPIHVAEYM
LDAARKLQPN LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAADSHEE GRLVYRYGGE
PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM ASCASGSTKG
YDELVPHQIS VVSEERFYTK WNPEALPSNT GEVNFQSGII AARRAINKLH QELGAKGFIQ
VYVDQVDQDI VAVTRHSPSI HQSVVSVSRT AFRNPKTSFY SKEVPHMYIP GKIEEVVLEA
RTIERHTIPY KKDENSINGM PDITVEIREH IQLNESKIVK HAGIVTKGPN EFVQEIEFEN
LTPGSVIIFR VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVDNADPILK IPFASSLYFL
ILSIASKLTL AELNQVLYRC ESEEQEDGGG CYNIPNWSSL KYAGLQGLMS ILAEIRPRND
LGHPFCDNLR SGDWMIDYVS SRLISRSGTI AEVGKWLQAM FLYLKQIPRY LIPCYFDAIL
IGAYTTLLDI AWKQMSSFVQ NGSTFVKHLS LGSVQMCGVG KFPSLPLLSP SLTDLPYRVN
EITKEKEQCC GSLAAGLPHF SAGIFRCWGR DTFIALRGLL LVTGRYLEAR NIILAFAGTL
RHGLIPNLLG EGTHARYNCR DAVWWWLQCI QDYCKIVPNG LDILRCPVSR MYPTDDSVPL
SAGTVDQPLF EVIQEAMQRH VQGIQFRERN AGPQIDRNMK DEGFNITAGV DEETGFVYGG
NRFNCGTWMD KMGESDRARN RGIPATPRDG SAVEIVGLSK SAVRWLLELS RKNIFPYHEV
RVKRHGKFVT VSYDEWNRKI QDNFEKLFHV SEDPSDFNEK HPELVHKRGI YKDSYGASSP
WCDYQLRPNF TIAMVVAPEL FTPEKAWKAL EIAEKKLLGP LGMKTLDPDD MVYCGIYDNA
LDNDNYNLAK GFNYHQGPEW LWPTGYFLRA KLYFSKLMGP ETNAKTMFLV KNVLSRHYVH
LERSPWKGLP ELTNENGQYC PFSCETQAWS IATVLETLYD L
//
